Comparison of the plaque assay and 50% tissue culture infectious dose assay as methods for measuring filovirus infectivity

Smither, Sophie J.; Lear-Rooney, Calli M.; Biggins, Julia E.; Pettitt, Jamie; Lever, Mark S.; Olinger, Gene G.

doi:10.1016/j.jviromet.2013.05.015

Cited by 114 publications

(80 citation statements)

References 14 publications

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“…This assay was selected because of its higher sensitivity and cost-effectiveness compared with plaque assay [17,18]. Virus liquid was sequentially diluted by minimum essential medium (MEM) in gradient dilutions from 10 −1 to 10 −10 and then cultured in 96-well culture plates (8 × 12 wells).…”

Section: Methodsmentioning

confidence: 99%

Enterovirus 71 Neutralizing Antibodies Seroepidemiological Research among Children in Guangzhou, China between 2014 and 2015: A Cross-Sectional Study

Zhang

Yan

Chen

et al. 2017

IJERPH

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A hand-foot-mouth disease outbreak occurred in 2014 around Guangdong. The purpose of this study was investigating the status and susceptibility of infectious neutralizing antibodies to enterovirus 71 among children so as to provide scientific evidence for the population immunity level of hand-foot-mouth disease and prepare for enterovirus 71 vaccination implementation. Serum specimens were collected from children in communities from January 2014 to March 2015 in Guangzhou. A total of 197 serum samples from children 1–5 years old were collected for this cross-sectional study via non-probabilistic sampling from the database of Chinese National Science and Technique Major Project. Neutralization activity was measured via micro neutralization test in vitro. The positive rate of enterovirus 71 neutralizing antibodies was 59.4%, whereas the geometric mean titre was 1:12.7. A statistically significant difference in true positive rates was found between different age groups but not between different genders. Being the most susceptible population of hand–foot–mouth disease, children under 3 years of age are more likely to be infected with enterovirus 71, and the immunity of children increases with increasing age. Further cohort studies should be conducted, and measures for prevention and vaccination should be taken.

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Section: Methodsmentioning

confidence: 99%

Enterovirus 71 Neutralizing Antibodies Seroepidemiological Research among Children in Guangzhou, China between 2014 and 2015: A Cross-Sectional Study

Zhang

Yan

Chen

et al. 2017

IJERPH

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“…The antiviral activity of WPOS, FOS and LOS extract were studied in the context of three main treatments which are post-, pre-and virucidal effect using plaque reduction assay. Plaque reduction assay is a quantitative measurement of the number of plaques that are formed by a virus at various dilutions (Smither et al 2013).…”

Section: Discussionmentioning

confidence: 99%

Antiviral Properties of Orthosiphon stamineus Aqueous Extract in Herpes Simplex Virus Type 1 Infected Cells

Ripim

Fazil

Ibrahim

et al. 2018

JSM

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A study was conducted to investigate the antiviral activity of aqueous extracts from Orthosiphon stamineus (OS). Extraction was done using different parts of OS. The whole plant except root (WPOS), leaves (LOS) and flowers (FOS) of OS were extracted using aqueous extraction method. Cytotoxicity was assessed using 3-(4,5-dimethylthiazol-2,5-diphenyl tetrazolium bromide (MTT) assay. Plaque reduction assays were carried out to evaluate the antiviral activity of OS extract against herpes simplex virus type 1 (HSV-1). These include post-treatment, pre-treatment and virucidal assays. High antiviral activity was observed in post-treatment and virucidal assay with 100% reduction of HSV-1 plaque at 0.39 mg/ mL in LOS, FOS and WPOS. In pre-treatment assay, 79%, 84% and 97% plaque reduction using the same concentration was observed in FOS, LOS and WPOS, respectively. In conclusion, this study showed that OS aqueous extract has promising potential to be explored as anti-HSV-1 agent regardless of the mode of treatment.

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“…One issue to take note of in animal model development of filovirus infection is the impact of particle to plaque-forming unit (PFU) ratios on lethality, wherein it is possible that increasing the dose could actually decrease infectivity due to an immunogenic effect produced by inactive virions in the stock. Additionally, the plaque assay used to measure live virions in a stock may greatly underestimate the true quantity of infectious virions in a preparation (Alfson et al, 2015;smither et al, 2013a).…”

Section: Filovirusesmentioning

confidence: 99%

Animal Models of Human Viral Diseases

Ruiz

Zumbrun

Nalca

2017

Animal Models for the Study of Human Disease

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Comparison of the plaque assay and 50% tissue culture infectious dose assay as methods for measuring filovirus infectivity

Cited by 114 publications

References 14 publications

Enterovirus 71 Neutralizing Antibodies Seroepidemiological Research among Children in Guangzhou, China between 2014 and 2015: A Cross-Sectional Study

Enterovirus 71 Neutralizing Antibodies Seroepidemiological Research among Children in Guangzhou, China between 2014 and 2015: A Cross-Sectional Study

Antiviral Properties of Orthosiphon stamineus Aqueous Extract in Herpes Simplex Virus Type 1 Infected Cells

Animal Models of Human Viral Diseases

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