Comparison of the properties of five pox virus strains isolated from monkeys

Abstract

Five strains of monkey pox viruses were compared with respect to their cultural characteristics in primary and continuous cell cultures and the lesions developed in embryonated eggs and in rabbit skin as well as to their hemagglutinating activity.Four strains ("Copenhagen"; "65-31"; "65-32" and "7-61") appeared to be similar in their properties. The cytopathogenic effect (CPE) was identical to that induced by vaccinia virus. There was no detectable virus multiplication in an pig kidney cell line (PEK). All four strains produced small, white, compact, hemorrhagic pock-like lesions on the chorioallantoic membrane.The strain "64-7275", isolated from healthy monkeys kidneys, had all properties of variola virus. It multiplied in the PEK cell line with a CPE. The lesions on the CAM were more compact without hemorrhage. In rabbit skin no detectable reaction occurred after infection with this strain.

Monkeypox virus.

“…CPE has not been observed in our laboratory in Hep-2 cells, certain lines of HeLa cells, and chicken embryo cell cultures (66), although others (48,70) have reported CPE in these cell cultures. Marennikova et al (48) reported that a strain of MPV "64-7275" had all the properties of variola virus and was unable to replicate in a continuous line of pig embryonic kidney cells which supported four other strains of MPV.…”

“…These cultured cells include primary and secondary lines of kidney cells derived from rhesus, cynomolgus, and African green monkeys (20,49,66,70); cells from rabbit, bovine (49,66) and guinea pig kidneys (48); mouse liver cells (66); and some of human origin, i.e., amnionic and human lung fibroblasts (31,48,66,70). CPE has not been observed in our laboratory in Hep-2 cells, certain lines of HeLa cells, and chicken embryo cell cultures (66), although others (48,70) have reported CPE in these cell cultures. Marennikova et al (48) reported that a strain of MPV "64-7275" had all the properties of variola virus and was unable to replicate in a continuous line of pig embryonic kidney cells which supported four other strains of MPV.…”

Labordifferenzierung der Orthopockenviren

“…Die Flaumfedern der lateralen Seite des rechten Beines wurden ausgezupft und anschliei3end in die Hautflache mittels einer Pipettenspitze 0,05 ml des Impfmaterials intensiv eingerieben. (1,3,7,9,15,17,20,28,30). Sieht man von den nicht immer auftretenden zentralen Nekrosen bei den V-Stammen, den nicht regelmai3igen hamorrhagischen Tendenzen und gewissen durchschnittlichen Groi3enschwankungen bei den V-, KP-, BP-und EP-Stammen ab, dann liei3en sich anhand der Herdmorphologie auf der CAM nur AP und CP von den anderen Arten spontan unterscheiden.…”

“…Der sonst typisch reagierende Stamm AP-3 liei3 sich in HEF-Kulturen nur schwer und ohne cpE vermehren. Bei AP-Stammen, die die charakteristischen Variola-ahnlichen CAM-Herde hervorriefen (7, 26, 30), wurde einmal eine hamorrhagische Komponente beschrieben (30). Die gleichen Stamme, Copenhagen (AP-l), ,,65-31" (AP-4), ,,65-32" (AP-2) und ,,64-7275" (AP-3) verhielten sich aber in den eigenen Untersuchungen nicht abweichend von der AP-ty ischen nicht…”

Monkeypox Virus as a Source of Whitepox Viruses

“…During 1968-1970 among the strains re ceived by us for assay as monkeypox viruses, two isolates from monkey kidney cell cul ture were detected which differed strikingly from the others and which appeared to be identical by laboratory markers to variola virus [1][2][3]. Later, four similar viruses were isolated by us from kidneys of wild monkeys and rodents captured in Central Africa in areas where human cases of monkeypox had occurred [3][4][5][6][7].…”