Comparison of the quantification of acetaminophen in plasma, cerebrospinal fluid and dried blood spots using high-performance liquid chromatography–tandem mass spectrometry

Taylor, Ryan W.; Hoffman, Keith L.; Schniedewind, Björn; Clavijo, Claudia F.; Galinkin, Jeffrey L.; Christians, Uwe

doi:10.1016/j.jpba.2013.04.007

Cited by 39 publications

(18 citation statements)

References 24 publications

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“…The essential part of all research on the application of DBS samples should be a comparison of the levels of tested analyte(s) between routinely used types of sample (most often liquid) and DBS samples and a search for possible correlations in the results. The fi nding of strong correlations indicates that DBS samples can be used as an effective alternative to sample collection (7,(67)(68)(69).…”

Section: Dbs and Liquid Sample Results Comparisonmentioning

confidence: 99%

The dried blood spot sampling method in the laboratory medicine

Andrlová¹,

Kanďár²

2019

BLL

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The minimization is currently expanding in all fi elds of human life. The smaller size of analytical instruments and their higher sensitivity enables an analysis of small amounts of a biological material with a very low consumption of chemicals, what is economically and also environmentally benefi cial. A patient friendly and minimal invasive sample collection is therefore more than required. The dried blood spot (DBS) sampling standardly used in the newborn screening (NS) may be an option. The sample collection is simple, non-invasive, does not require trained medical personnel assistance and also storing and transportation of these samples is much easier in comparison with the whole blood samples. The DBS sampling method is used in the therapeutic drug monitoring or in the diagnosis of infectious diseases and its usage is still spreading. In some cases, it is still only a research object, but it shows a big potential for a future use. It could completely replace the whole venous blood collection in some cases, for example in the metabolic screening of diabetic patients or monitoring of treatment response, and it can overall simplify the sample collection and the transportation process (Tab. 1, Ref. 152).

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Section: Dbs and Liquid Sample Results Comparisonmentioning

confidence: 99%

The dried blood spot sampling method in the laboratory medicine

Andrlová¹,

Kanďár²

2019

BLL

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“…Most families consented to an optional fourth blood sample obtained just prior to a methadone dose after the participant failed to respond to treatment as anticipated. Concentrations of methadone and its biologically inactive metabolites in dried blood spots on Guthrie cards were determined using a high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay at the University of Colorado, Aurora, CO, as previously published 24–26 . The lower limit of quantitation was 0.25 ng/mL for methadone and 0.1 ng/mL for 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) and 2-ethyl-5-methyl-3,3-diphenyl-1-pyrroline (EMDP).…”

Section: Methodsmentioning

confidence: 99%

Pharmacokinetics of Oral Methadone in the Treatment of Neonatal Abstinence Syndrome: A Pilot Study

Wiles

Isemann

Mizuno

et al. 2015

The Journal of Pediatrics

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Objective To characterize the population pharmacokinetic (PK) of oral methadone in neonates requiring pharmacologic treatment of neonatal abstinence syndrome (NAS) and to develop a PK model towards an evidence-based treatment protocol. Study design Based on a methadone dosing protocol, serum concentrations of methadone and its metabolites were assessed via high performance liquid chromatography-tandem mass spectrometry from dried blood spots. Population PK analysis was performed to determine the volume of distribution and clearance of oral methadone. Methadone plasma concentration-time profiles were simulated from the deduced PK model to optimize the dosing regimen. Results There was substantial inter-individual variability in methadone concentrations. Blood concentrations of methadone were best described by a one-compartment model with first-order absorption. The population mean estimates (coefficient of variation percentage) for oral clearance and volume of distribution were 8.94 (103%) L/h/70 kg and 177 (133%) L/70 kg, respectively. Optimized dosing strategies were developed based on the simulated PK profiles. We suggest a starting dose of 0.1 mg/kg per dose every 6 hours for most patients requiring pharmacologic treatment of NAS followed by an expedited weaning phase. Conclusions The proposed dosing regimen may reduce the cumulative dose of opioid and shorten the length of hospitalization. Future studies should aim to validate the simulated dosing schemes with clinical data and expand our understanding of the between-patient PK variability. Trial registration ClinicalTrials.gov: NCT01754324

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“…Only few works were traceable for comparative PK evaluation of DBS and evaluation of DBS and plasma. 14,[20][21][22] Our present study has an advantage to have samples from PK study for assessment of HCTZ concentrations in two different matrices that were simultaneously collected at the same time points. This gives the advantage of the present assay being developed and validated in two different matrices.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of dried blood spot technique for pharmacokinetic study of hydrochlorothiazide and comparison of human plasma versus dried blood spot

PavanKumara¹,

Murthy²

2014

J Young Pharm.

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Background: The dried blood spot (DBS) technique is a convenient alternative sample collection method to venipuncture. The use of DBS was fi rst widely introduced in the 1960's by Dr. Guthrie to measure levels of phenylalanine in newborns for the diagnosis of phenylketonuria. The scientifi c community has recently expressed interest in applying the DBS technique for quantifi cation of drugs in biological fl uids. Materials and Methods: Reversed phase high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) assays for the determination of hydrochlorothiazide (HCTZ) in human plasma and DBS samples were developed and validated. Furthermore, clinical samples collected from human volunteers as a part of pharmacokinetic (PK) study and PK data from plasma were compared against DBS. Results: The range of reliable response in plasma and DBS was 2-200 ng/mL with a correlation coeffi cient ≥0.997. Inter-day accuracy was always within 85-115%, and inter-day precision for plasma and DBS were <15%. Conclusions: Comparison of HCTZ PK data in DBS and plasma yielded good correlation, indicating that DBS can be used as a plasma alternative for PK analyses in vivo.

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Comparison of the quantification of acetaminophen in plasma, cerebrospinal fluid and dried blood spots using high-performance liquid chromatography–tandem mass spectrometry

Cited by 39 publications

References 24 publications

The dried blood spot sampling method in the laboratory medicine

The dried blood spot sampling method in the laboratory medicine

Pharmacokinetics of Oral Methadone in the Treatment of Neonatal Abstinence Syndrome: A Pilot Study

Evaluation of dried blood spot technique for pharmacokinetic study of hydrochlorothiazide and comparison of human plasma versus dried blood spot

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