Comparison of the susceptibilities of clinical isolates of Candida albicans and Candida dubliniensis to essential oils

Abstract

Here, a microdilution technique based on the M27-A2 protocol (NCCLS, 2002) was employed to compare the susceptibilities of Candida albicans and Candida dubliniensis to essential oils extracted from plants used as spices. The chemical compositions of the essential oils were defined based on the analysis of retention indices obtained by gas chromatography-mass spectroscopy. Taken together, the results showed that the activity of the compounds against the two species was similar.

Antifungal activity of components used for decontamination of dental prostheses on the growth of Candida albicans

“…The objective of the agar diffusion test was to identify which products presented the potential to inhibit the studied microorganism, without establishing a comparison between the measurments of the inhibiton zone, due to the different diffusion speeds of the products tested and the possibility of interference by the Sabouraud-Dextrose culture medium with substance diffusion. 20 In addition, the low sensitivity of the agar diffusion test resulted in differences in antifungal activity for some products in two microbiological tests (Tables 2 and 4). …”

Susceptibilities of Candida albicans Mouth Isolates to Antifungal Agents, Essentials Oils and Mouth Rinses

“…In this study, cinnamon was the most efficient oil. Previous studies recognize that cinnamon oil is an efficient antifungal or anti-candidal agent [36,37,[40][41][42] and have interest as a source of natural products for potential use as alternative drugs to heal many infectious diseases [43][44][45]. Besides cinnamon, other essential oils exhibited high efficacy, as laurel and mint.…”

Perfil de suscetibilidade de leveduras do gênero Candida isoladas de animais ao óleo essencial de Rosmarinus officinalis L

“…A infecção oportunista em animais desenvolve-se como resultado da quebra dos mecanismos de defesa inata, imunossupressão e antibioticoterapia prolongada, entre outros fatores (Moretti et al, 2006;Cleff et al, 2007;Ong et al, 2010;Yurayart et al, 2011). Atualmente, candidíase representa um problema de saúde pública, com resistência de isolados aos antifúngicos convencionais, e relatos de recidivas da enfermidade em especial nos pacientes imunocomprometidos (Sanglard & Odds, 2002;Colombo & Guimarães, 2003;Pozzatti et al, 2010).…”

“…Além disso, estes fármacos apresentam efeitos adversos e custo relativamente elevado, especialmente quando considerado o uso em animais (Sanglard & Odds, 2002;Mondelo et al, 2003;Pressler et al, 2003;Rochete et al, 2003;Giordani et al, 2004;Luqman et al, 2007).…”

“…A ação antimicrobiana do alecrim, tanto na forma de extratos como de óleo essencial, tem sido avaliada principalmente em bactérias dos gêneros Staphylococcus, Streptococcus, Proteus, Pseudomonas, Klebsiella, Salmonella, Mycobacterium e Escherichia (Porte & Godoy, 2001;Angioni et al, 2004;Santoyo et al, 2005;Celiktas et al, 2007;Luqman et al, 2007;Bernardes et al, 2010;Hussain et al, 2010 Schwiertz et al, 2006;Celiktas et al, 2007;Luqman et al, 2007;Pozzatti et al, 2010;Cavalcanti et al, 2011) não sendo encontradas na literatura consultada, pesquisas que avaliem o efeito do óleo essencial do alecrim em C. albicans isolada de mucosa e casos clínicos em animais de companhia e silvestres. Nesse sentido, o presente estudo teve como objetivos identificar os principais constituintes químicos, usando a cromatografia gasosa no óleo essencial de alecrim cultivado em Pelotas/RS e, avaliar as propriedades antifúngicas do óleo essencial em Candida albicans isoladas da microbiota e de casos clínicos de candidíase em animais, assim como em isolados padrões de Candida spp.…”