Comparison of the therapeutic effects of adipose‑derived and bone marrow mesenchymal stem cells on erectile dysfunction in diabetic rats

Chen, Sansan; Zhu, Jianbin; Wang, Mingzhu; Huang, Yan-ting; Qiu, Zhuolin; Li, Jingjing; Chen, Xinglu; Xu, Mingyu; Li, Jun; She, Miaoqin; Li, Hongwei; Yang, Xiaorong; Wang, Yichuan; Cai, Xiangsheng

doi:10.3892/ijmm.2019.4254

Cited by 19 publications

(19 citation statements)

References 45 publications

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“…To date, adipose-derived mesenchymal stem cell (ADSC) is considered as one of the most frequent cells used in the field of uro-andrology because it is abundant and easy to obtain[ 39 - 41 ]. Moreover, ADSCs have anti-inflammatory properties and an ability to repair vascular and nerve damage, which are the hallmarks in the development of diabetic ED[ 42 ].…”

Section: Stem Cells As a Future Therapy For Diabetic Edmentioning

confidence: 99%

Stem cell therapy and diabetic erectile dysfunction: A critical review

Pakpahan¹,

Ibrahim²,

William³

et al. 2021

WJSC

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Erectile dysfunction (ED) has been identified as one of the most frequent chronic complications of diabetes mellitus (DM). The prevalence of ED is estimated to be about 67.4% in all DM cases worldwide. The pathophysiological process leading to ED involves endothelial, neurological, hormonal, and psychological factors. In DM, endothelial and neurological factors play a crucial role. Damages in the blood vessels and erectile tissue due to insulin resistance are the hallmark of ED in DM. The current treatments for ED include phosphodiesterase-5 inhibitors and penile prosthesis surgery. However, these treatments are limited in terms of just relieving the symptoms, but not resolving the cause of the problem. The use of stem cells for treating ED is currently being studied mostly in experimental animals. The stem cells used are derived from adipose tissue, bone, or human urine. Most of the studies observed an improvement in erectile quality in the experimental animals as well as an improvement in erectile tissue. However, research on stem cell therapy for ED in humans remains to be limited. Nevertheless, significant findings from studies using animal models indicate a potential use of stem cells in the treatment of ED.

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Section: Stem Cells As a Future Therapy For Diabetic Edmentioning

confidence: 99%

Stem cell therapy and diabetic erectile dysfunction: A critical review

Pakpahan¹,

Ibrahim²,

William³

et al. 2021

WJSC

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“…However, the proliferation ability of BMSCs was limited, especially in older patients. Adipose-derived stem cells (ADSCs) could be a potential alternative for BMSCs due to their advantages of being easy to obtain and widely sourced [5,6].…”

Section: Introductionmentioning

confidence: 99%

Long noncoding RNA LINC00314 facilitates osteogenic differentiation of adipose-derived stem cells through the hsa-miR-129-5p/GRM5 axis via the Wnt signaling pathway

Zhang

Fan

et al. 2020

Stem Cell Res Ther

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Background: Many studies have shown that long noncoding RNAs (lncRNAs) are closely related to the stimulation of osteogenic differentiation of adipose-derived stem cells (ADSCs) and the prevention of osteoporosis. Current research aimed to investigate the novel lncRNA and explored the function and molecular mechanism of the LINC00314/miR-129-5p/GRM5 axis in regulating osteogenic differentiation of ADSCs. Methods: LncRNA and miRNA sequencing was performed in normal and osteogenic differentiation-induced ADSCs (osteogenic group). Abnormally expressed lncRNAs and miRNAs were obtained by the R software and the relative expression of LINC00314, miR-129-5p, and GRM5 during osteogenic induction was measured by RT-PCR. ADSCs were then transfected with pcDNA3.1-sh-LINC00314 and agomiR-129-5p. Alizarin red staining (ARS) and alkaline phosphatase (ALP) staining were performed to identify the mechanism of the LINC00314/miR-129-5p/GRM5 axis in regulating osteogenic differentiation of ADSCs. Results: LINC00314 was significantly upregulated in the group of osteogenic-induced ADSCs. LINC00314 and GRM5 mimics increased the early and late markers of osteogenic differentiation, which manifest in not only the markedly increased ALP activity but also higher calcium deposition, while miR-129-5p mimic had the opposite effects. LINC00314 directly targeted miR-129-5p through luciferase reporter assay, and miR-129-5p suppressed GRM5 expression. Moreover, the LINC00314/miR-129-5p/GRM5 regulatory axis activated the Wnt/β-catenin signaling pathway. Conclusions: LINC00314 confers contributory function in the osteogenic differentiation of ADSCs and thus the LINC00314/miR-129-5p/GRM5 axis may be a novel mechanism for osteogenic-related disease.

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“…Significant number of sinusoids was observed as locating adjacent to the outer regions of CC which are close to the tunica albuginea. Several histological studies have been performed for CC and sinusoids by utilizing thin sections with H&E staining 41,42 . Histological analyses with such thin sections of H&E staining generally revealed only part of the CC structures thus not revealing the entire 3D structures 43‐45 .…”

Section: Discussionmentioning

confidence: 99%

3D reconstruction and histopathological analyses on murine corporal body

Hashimoto

Kajimoto

Ueda

et al. 2021

Reprod Medicine & Biology

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Purpose Erectile dysfunction (ED) is one of the increasing diseases with aging society. The basis of ED derived from local penile abnormality is poorly understood because of the complex three‐dimensional (3D) distribution of sinusoids in corpus cavernosum (CC). Understanding the 3D histological structure of penis is thus necessary. Analyses on the status of regulatory signals for such abnormality are also performed. Methods To analyze the 3D structure of sinusoid, 3D reconstruction from serial sections of murine CC were performed. Histological analyses between young (2 months old) and aged (14 months old) CC were performed. As for chondrogenic signaling status of aged CC, SOX9 and RBPJK staining was examined. Results Sinusoids prominently developed in the outer regions of CC adjacent to tunica albuginea. Aged CC samples contained ectopic chondrocytes in such regions. Associating with the appearance of chondrocytes, the expression of SOX9, chondrogenic regulator, was upregulated. The expression of RBPJK, one of the Notch signal regulators, was downregulated in the aged CC. Conclusions Prominent sinusoids distribute in the outer region of CC which may possess important roles for erection. A possibility of ectopic chondrogenesis induced by alteration of SOX9/Notch signaling with aging is indicated.

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Comparison of the therapeutic effects of adipose‑derived and bone marrow mesenchymal stem cells on erectile dysfunction in diabetic rats

Cited by 19 publications

References 45 publications

Stem cell therapy and diabetic erectile dysfunction: A critical review

Stem cell therapy and diabetic erectile dysfunction: A critical review

Long noncoding RNA LINC00314 facilitates osteogenic differentiation of adipose-derived stem cells through the hsa-miR-129-5p/GRM5 axis via the Wnt signaling pathway

3D reconstruction and histopathological analyses on murine corporal body

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