Comparison of the use of morphological, protein and DNA markers in the genetic characterization of Iranian wild Prunus species

Zeinalabedini, Mehrshad; Majourhat, Khalid; Khayam-Nekoui, M.; Grigorian, V.; Torchi, M.; Dicenta, F.; Martínez-Gómez, P.

doi:10.1016/j.scienta.2007.10.022

Cited by 40 publications

(27 citation statements)

References 29 publications

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“…This result was expected since almond is an outbreeder and spontaneous hybridization is known to occur among species (Serafinov 1971;Denisov 1988). This high phenotypic variability agree with previous reports in the molecular characterization using different markers as nuclear and chloroplast Simple sequence repeats (SSRs) Zeinalabedini et al 2008) or Amplified fragment length polymorphisms (AFLPs) (Sorkheh et al 2007).…”

Section: Diversity Indices Principal Component Analysis and Correlatsupporting

confidence: 92%

Phenotypic diversity within native Iranian almond (Prunus spp.) species and their breeding potential

Sorkheh

Shiran

Rouhi

et al. 2009

Genet Resour Crop Evol

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A total of 137 accessions from 18 wild almond species were collected from Iran and leaf and fruit traits were characterized. Also evaluated were flowering and ripening date, self-incompatibility and kernel bitterness. An extensive phenotypic diversity was found both among and within species. Differences in average leaf dimensions among and within species were associated with average rainfall but not altitude of collection site. Adjacent accessions located in drier areas had smaller leaf dimensions than those located in semi-humid or humid regions. No relation was found between average fruit dimensions and collection site conditions. Principal component analysis revealed that the nut weight and width, and the kernel weight had highest loading in the first component accounting for 45.8% of total variation. In contrast, leaf traits in the second component accounted for 22.3% of total variation. No significant correlations were detected between leaf dimensions and fruit traits in all species evaluated. Results document a rich source of new germplasm for almond improvement programs. Small fruit size, pollen-pistil self-incompatibility, and bitter kernel flavour are the most common obstacles to the utilization of this wild germplasm in breeding.

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Section: Diversity Indices Principal Component Analysis and Correlatsupporting

confidence: 92%

Phenotypic diversity within native Iranian almond (Prunus spp.) species and their breeding potential

Sorkheh

Shiran

Rouhi

et al. 2009

Genet Resour Crop Evol

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“…Morphological characters were used in phenotypic observations to characterize the genetic diversity of almond species, but their interactions with the environment and the small number of characters [20][21][22] prompted the use of other more discriminating techniques. Currently, DNA markers are widely used in studies of genetic diversity and the clarification of certain research questions, among others, those concerning their genetic origin [9].…”

Section: Introductionmentioning

confidence: 99%

Assessment of Genetic Diversity of Moroccan Cultivated Almond (<i>Prunus dulcis</i> Mill. DA Webb) in Its Area of Extreme Diffusion, Using Nuclear Microsatellites

Elhamzaoui¹,

Oukabli²,

Charafi³

et al. 2012

AJPS

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Assessment of genetic diversity of Moroccan cultivated almond (Prunus dulcis Mill.) grown from seed and cultivated at four eco-geographical regions was performed using 16 nuclear SSRs. 238 alleles were detected with an average of 14.88 alleles per locus, ranging from 4 (locus BPPCT027) to 24 (locus CPSCT018). The size of alleles ranged from 84 bp (locus UDP96-003) to 253 bp (locus UDP96-018). A high genetic diversity of the local almonds is apparent and structured into three major clusters (Oasis cluster, High and Anti Atlas cluster, and Middle Atlas cluster). Compared to the Mediterranean genetic pools, from the East to West, the genetic diversity tends to be limited in Morocco which is the area of its extreme diffusion.

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“…This tendency has nothing to do with the known dissemination routes of almonds from Central Asia to the Mediterranean, which could be explained by the discrepancies described above. However, genetic relationships calculated from the proportion of shared alleles gave a good reflection of the closer genetic relationships between Spanish, French, Italian and Iranian cultivars in comparison with Ukrainian, Slovakian and Czech cultivars (Zeinalabedini et al, 2008). Distinct clustering was also apparent for Portuguese and Tunisian cultivars.…”

Section: Discussionmentioning

confidence: 93%

“…Nevertheless, the utilization of isoenzyme detection is limited by the small 382 number of loci that can be analysed with relatively simple enzyme staining methods, as well as the low levels of allelic variation at most loci. Interestingly, as a biochemical parameter the total protein content seemed to be accurately correlated with the taxonomical relationships between various wild relatives of almond (Zeinalabedini et al, 2008).…”

Section: Isoenzyme Detectionmentioning

confidence: 99%

Review of genetic diversity studies in almond (Prunus dulcis)

Szikriszt

Hegedűs

Halász

2011

Acta Agronomica Hungarica

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Almond [Prunus dulcis (Mill.) D.A. Webb.] is cultivated for its nuts and is one of the oldest domesticated plants. Due to the genetically controlled self-incompatibility system that operates in this species, almond is characterized by great genetic diversity, the estimation of which has benefited from a range of marker techniques, including morphological characteristics, isoenzyme detection and molecular markers. Among the DNA-based molecular markers, simple sequence repeats (SSR) have been used most widely, although analyses have ranged from restriction fragment length polymorphism to the most recent single nucleotide polymorphism detection methods. Molecular markers have also been used to trace specific agronomic traits, e.g. self-(in)compatibility or kernel bitterness. Genetic diversity studies in almond have not revealed a direct relationship between the level of diversity and the origin of the germplasm. This might be explained by the relatively recent occurrence of self-compatibility in almond, which has not yet caused a serious loss of genetic diversity. The markers reviewed will be useful in monitoring and maintaining genetic diversity in almond breeding programmes, while others may permit marker-assisted selection for favourable agronomic traits. The cultivation, breeding and conservation of wild-growing almonds may equally benefit from the genetic diversity studies (especially those applying molecular markers).

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Comparison of the use of morphological, protein and DNA markers in the genetic characterization of Iranian wild Prunus species

Cited by 40 publications

References 29 publications

Phenotypic diversity within native Iranian almond (Prunus spp.) species and their breeding potential

Phenotypic diversity within native Iranian almond (Prunus spp.) species and their breeding potential

Assessment of Genetic Diversity of Moroccan Cultivated Almond (<i>Prunus dulcis</i> Mill. DA Webb) in Its Area of Extreme Diffusion, Using Nuclear Microsatellites

Review of genetic diversity studies in almond (Prunus dulcis)

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Comparison of the use of morphological, protein and DNA markers in the genetic characterization of Iranian wild Prunus species

Cited by 40 publications

References 29 publications

Phenotypic diversity within native Iranian almond (Prunus spp.) species and their breeding potential

Phenotypic diversity within native Iranian almond (Prunus spp.) species and their breeding potential

Assessment of Genetic Diversity of Moroccan Cultivated Almond (&lt;i&gt;Prunus dulcis&lt;/i&gt; Mill. DA Webb) in Its Area of Extreme Diffusion, Using Nuclear Microsatellites

Review of genetic diversity studies in almond (Prunus dulcis)

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Product

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Assessment of Genetic Diversity of Moroccan Cultivated Almond (<i>Prunus dulcis</i> Mill. DA Webb) in Its Area of Extreme Diffusion, Using Nuclear Microsatellites