Comparison of the VIDAS RSV assay and the Abbott Testpack RSV with direct immunofluorescence for detection of respiratory syncytial virus in nasopharyngeal aspirates

Abstract

The sensitivity and accuracy of the VIDAS RSV assay in testing fresh specimens were 82.7 and 87.1%, respectively, whereas specimens previously frozen at-70°C gave a sensitivity of 96.2% and an accuracy of 95.4%. The sensitivity and accuracy of Abbott Testpack RSV were 92.6 and 91.3% for fresh specimens and 86.8 and 88.1% for frozen specimens. The advantages and drawbacks of the two assays are discussed.

Microwave-accelerated direct immunofluorescent staining for respiratory syncytial virus and influenza A virus

“…Of the 319 specimens tested, the Abbott Test Pack RSV test missed 11 of the 88 positive specimens detected by DFA. This result is consistent with previous reports (5,8,9,11,16) that EIA is less sensitive than DFA or IF assay. The DFA for influenza A virus was not compared with other rapid methods such as EIA.…”

Assessment of direct immunofluorescence assay in detection of antiviral effect of garlic extract on influenza virus

“…The activity of possible and potential antiviral compounds may be assessed in vitro by measuring their ability to inhibit a stage or enzymatic activity in viral replication cycle (Takeda et al, 2002;Lei et al, 2011). Immunofluorescent (IF) assay has been recommended for rapid in vitro and in vivo detection of respiratory viruses including influenza virus (Minnich and Ray, 1980;Ray and Minnich, 1987;Takimoto et al, 1991;Miller et al, 1993;Pianciola et al, 2010). Detection of antigen by fluorescein-labeled antibody was first described by Coons et al (Coons et al, 1941).…”

Variabilidade genética da proteína G do HRSV de amostras com o genótipo BA.

“…O Test Pack é um ensaio imunoenzimático em membrana de nitrocelulose utilizado para a detecção de antígenos do HRSV. Disponível em forma de Kit comercial (Abbott, North Chicago, Illinois, EUA), o Test Pack é um método de diagnóstico rápido de HRSV, sendo utilizado na triagem de pacientes, porém é menos sensível do que a imunofluorescência (MILLER et al, 1993).…”