Comparison of three different anionic surfactants for the separation of hydrophobic compounds by nonaqueous capillary electrophoresis

Abstract

The effect of the three different surfactants, sodium dodecyl sulfate (SDS), diethylhexyl sodium sulfosuccinate (AOT), and taurodexycholic acid sodium salt (STDC) on the nonaqueous capillary electrophoretic separations of hydrophobic compounds were compared with formamide containing 20 mM K2HPO4 as electrolyte solvent. Separations of all selected uncharged hydrophobic compounds, e.g., p-arylacetophones were shown to be strongly dependent on the kind of surfactant. The electrolyte containing 180 mM SDS provided the best result for the selected samples.

Self-assembled coating for modification of the electro-osmotic flow in nonaqueous capillary electrophoresis using formamide

“…Figure 2 shows that in pure FA 2C 18 DAB reverses the EOF, and that increased concentrations of surfactant gradually increase the magnitude of the EOF. This behavior is similar to our previous observations of C 16 TAB in MeOH-water under conditions where the zeta potential of the capillary was low (45).…”

“…Sahota and Khaledi (12) reported the first investigation of FA as a CE medium in 1994. Since then, FA or FA-aqueous buffers have been used for CE separations of enantiomers (13,14), for control of the electro-osmotic flow (EOF) (15), as a solvent in MEKC (16)(17)(18)(19), and for other applications (15,20,21). However, the potential of FA as a pure or mixed solvent in CE has not been fully achieved because of a lack of understanding of the influence of the solvent on mobility, low absorbance detection sensitivity since FA has a UV cut-off of 275 nm, and the slow EOF and mobilities in FA due to its high viscosity (3.3 cP).…”

Recent advances in nonaqueous capillary electrophoresis

“…The effect of different surfactants on the separations in NACE has been investigated in several recent studies. Lin et al [51] found sodium dodecyl sulfate to be a more suitable surfactant than two other surfactants that were studied, namely diethylhexyl sodium sulfosuccinate, and taurodeoxycholic acid sodium salt, in formamide containing 20 mM K 2 HPO 4 for the separation of several arylacetophenones.…”

Comparison of two capillary electrophoresis online stacking modes by analysis of polycyclic aromatic hydrocarbons in airborne particulates

“…Warner and coworkers [10] have successfully separated 16 PAHs [9] and 12 monomethyl-benz[a]anthracene isomers by exploring a high-purity T-type polymerized surfactant with an undecenyl (C 11 ) and a sulfate group. Moreover, several other additives were reported, and they included a bile salt surfactant [11], a high-concentration ionic surfactant [12], and a neutral and anionic CD mixture [13]. Among these techniques, CD-modified MEKC (CD-MEKC) has been demonstrated to be an efficient separation method for PAHs [14].…”