Comparison of Three Different Immunoassays and PCR for the Detection of Hepatitis C Virus Infection in Pregnant Women in Taiwan

Lin, Ho‐Hsiung; Kao, Jia‐Horng; Leu, Jer-Herng; Young, Yin-Chin; Lee, Tzu-Yao; Chen, Pei‐Jer; Chen, Ding‐Shinn

doi:10.1159/000462399

Cited by 5 publications

(7 citation statements)

References 25 publications

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“…We found among 2046 pregnant women a total of 42 (2.1%) viremic for GBV-C/HGV, a prevalence higher than that of HCV infection in the same population on Taiwan [19]. Furthermore, 52% (95% confidence interval, 32.4% -71.6%) of the viremic mothers transmitted GBV-C/HGV to their offsprings.…”

Section: Discussionmentioning

confidence: 79%

Mother‐to‐Infant Transmission of GB Virus C/Hepatitis G Virus: The Role of High‐Titered Maternal Viremia and Mode of Delivery

et al. 1998

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To study mother-to-infant transmission of GB virus C/hepatitis G virus (GBV-C/HGV), blood samples of infants born to carrier mothers were collected beginning 3 months after birth and were tested for GBV-C/HGV RNA until 1 year of age. Of 2046 mothers, 2.1% were positive for GBV-C/ HGV RNA, and 25 of their infants were followed for a median of 12 months. Thirteen infants (52%) were viremic, and infection became persistent in all. Maternal GBV-C/HGV RNA levels of this group were ú10 7 copies/mL. Nucleotide sequence comparison in 5 viremic mother-infant pairs revealed a homology of 93% -98.2%, and none delivered by elective cesarean section. In comparison, of the 12 uninfected infants' mothers, 10 had lower GBV-C/HGV RNA levels (mean, 5 1 10 4 copies/ mL), and the remaining 2 high-titered mothers had elective cesarean section. Thus, high-titered maternal viremia and mode of delivery are closely associated with the mother-to-infant transmission of GBV-C/HGV to infants, and the infection usually becomes persistent. for GBV-C/HGV RNA and alanine aminotransferase (ALT) startcan also be found in patients with community-acquired hepatiing from 3 months after birth until 1 year of age (usually every tis [14] and is distributed worldwide, with a higher prevalence 2-3 months). The infected neonates and blood sampling times are in certain risk groups [9 -15].shown in figure 1. The duration of follow-up was comparable between infected and uninfected infants. Although perinatal transmission of GBV-C/HGV has beenThe mode of delivery was classified into elective cesarean reported to occur in some instances [16 -18], the case number section, emergent cesarean section, and normal spontaneous delivis small, and thus the prevalence, risk factors, and significance ery [20]. remain unclear. We therefore investigated the frequency of Materials and Methodsrum GBV-C/HGV RNA was detected by reverse transcriptionSubjects. Serum samples of 2046 consecutive pregnant women polymerase chain reaction (RT-PCR) with primers from the 5-collected from May 1995 to May 1996 and stored at 020ЊC were untranslated region of the viral genome [21], and the positive maternal serum samples were further quantified. To screen the GBV-C/HGV genome, serum specimens from 10 pregnant women were pooled and then subjected to 21]. In case

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Section: Discussionmentioning

confidence: 79%

Mother‐to‐Infant Transmission of GB Virus C/Hepatitis G Virus: The Role of High‐Titered Maternal Viremia and Mode of Delivery

et al. 1998

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“…4 In fact, 16 patients in our study had groupspecific antibodies but did not have serum HCV RNA, suggesting a past infection of HCV or an extremely low level of viremia that escaped detection by the present PCR. 4 Of interest, we found that patients with group 1 antibodies had a higher rate of viremia than those with group 2 antibodies (92% vs 62%, P < 0.005), and this gave support to previous studies showing that patients with genotype lb HCV have a greater amount of virus than those with genotype 2a. 40"41 Thus, the serotyping assay is obviously a practical alternative to current PCR genotyping methods and remains the only possible test in those positive for anti-HCV but negative for HCV RNA.…”

Section: Discussionmentioning

confidence: 64%

Serotyping of hepatitis C virus in chronic type C hepatitis in Taiwan: Correlation with genotypes

et al. 1996

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To evaluate the usefulness of a new serologic assay to group hepatitis C virus (HCV), genotypes identified by this serotyping method were compared to those identified by a polymerase chain reaction (PCR) assay with type-specific primers in 71 Taiwanese patients with chronic type C hepatitis. The group-specific antibodies against different HCV genotypes were detected by using an enzyme-linked immunosorbent assay (ELISA) based on group-specific recombinant peptides (C14-1 and C14-2) within the NS4 region. Among 71 patients positive for current second-generation HCV antibodies, HCV RNA was detected in 55 patients by PCR with primers from the 5' untranslating region, and in 52 by genotype-specific PCR. In 49 (89%) of 55 viremic patients, the results of serotyping by ELISA showed complete agreement with those determined by PCR genotyping, and none of the patients showed a group opposite to that of HCV genotype. The positive rate of group-specific antibodies (69/71;97%) was even better than that of the PCR (55/71;78%). We conclude that this new serotyping assay is highly sensitive and specific for the determination of HCV genotypes, and will be useful in future epidemiologic studies, as well for clinical application.

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“…Since the development of first-generation sérologie assays (2), detection of anti-HCV has become the standard test for the diagnosis of HCV infection (3,9), and the presence of anti-HCV usually indicates infectivity (6,8,23). Accordingly, such tests have a major impact on screening of blood donors, which leads to a reduction in the incidence of posttransfusion non-A, non-B hepatitis (12).…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have shown that second-generation anti-HCV assays perform much better than first-generation assays in the detection of HCV infection because of the incorporation of a highly conserved core peptide of the virus (3,(5)(6)(7)(8). Thus second-generation anti-HCV tests are now widely used for clinical diagnosis and blood donor screening (9).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Third-Generation Hepatitis C Antibody Assay in Chronic Hepatitis C and Chronic Non-B, Non-C Hepatitis

Kao

Yang²,

Lai³

et al. 1995

Viral Immunology

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Second-generation hepatitis C virus (HCV) antibody assays are more sensitive and specific than first-generation assays in the detection of HCV infection; such tests are widely used. However, there are still HCV-infected patients who test seronegative for anti-HCV even by second-generation assays. In this study we evaluated the performance of the new third-generation HCV assay (HCV 3.0) in 230 individuals with different second-generation anti-HCV (HCV 2.0) and HCV RNA patterns. Our results showed the followings: only one healthy adult had a discrepant result in 200 subjects negative (group I) or positive (group II) for HCV 2.0 and HCV RNA; 7 of 13 (54%) HCV 2.0-negative but HCV RNA-positive patients (group III) were HCV 3.0-positive; two of 17 (12%) so-called chronic non-B, non-C hepatitis patients (group IV) were positive for HCV 3.0. We conclude that third-generation anti-HCV assays are more sensitive and specific than second-generation assays in the detection of chronic HCV infection; however false-positive results may be observed among low-risk healthy persons.

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Comparison of Three Different Immunoassays and PCR for the Detection of Hepatitis C Virus Infection in Pregnant Women in Taiwan

Cited by 5 publications

References 25 publications

Mother‐to‐Infant Transmission of GB Virus C/Hepatitis G Virus: The Role of High‐Titered Maternal Viremia and Mode of Delivery

Mother‐to‐Infant Transmission of GB Virus C/Hepatitis G Virus: The Role of High‐Titered Maternal Viremia and Mode of Delivery

Serotyping of hepatitis C virus in chronic type C hepatitis in Taiwan: Correlation with genotypes

Evaluation of Third-Generation Hepatitis C Antibody Assay in Chronic Hepatitis C and Chronic Non-B, Non-C Hepatitis

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