Comparison of three different methods for effective introduction of platelet-rich plasma on PLGA woven mesh

Abstract

For successful tissue regeneration, effective cell delivery to defect site is very important. Various types of polymer biomaterials have been developed and applied for effective cell delivery. PLGA (poly lactic-co-glycolic acid), a synthetic polymer, is a commercially available and FDA approved material. Platelet-rich plasma (PRP) is an autologous growth factor cocktail containing various growth factors including PDGF, TGFβ-1 and BMPs, and has shown positive effects on cell behaviors. We hypothesized that PRP pretreatment on PLGA mesh using different methods would cause different patterns of platelet adhesion and stages which would modulate cell adhesion and proliferation on the PLGA mesh. In this study, we pretreated PRP on PLGA using three different methods including simple dripping (SD), dynamic oscillation (DO) and centrifugation (CE), then observed the amount of adhered platelets and their activation stage distribution. The highest amount of platelets was observed on CE mesh and calcium treated CE mesh. Moreover, calcium addition after PRP coating triggered dramatic activation of platelets which showed large and flat morphologies of platelets with rich fibrin networks. Human chondrocytes (hCs) and human bone marrow stromal cells (hBMSCs) were next cultured on PRP-pretreated PLGA meshes using different preparation methods. CE mesh showed a significant increase in the initial cell adhesion of hCs and proliferation of hBMSCs compared with SD and DO meshes. The results demonstrated that the centrifugation method can be considered as a promising coating method to introduce PRP on PLGA polymeric material which could improve cell-material interaction using a simple method.

Oxidative Epigallocatechin Gallate Coating on Polymeric Substrates for Bone Tissue Regeneration

“…Fourier transform infrared (FTIR) spectroscopy analysis of nanofiber samples ( Figure 3 a) showed the presence of a broad band at 3100–3150 cm −1 , which can be attributed to O–H stretching vibrations of the phenyl hydroxyl groups present in the EGCG . Furthermore, a small peak at 1600 cm −1 corresponding to the C=C bond present in the EGCG structure also appeared, confirming successful coating of EGCG on the nanofiber surface.…”

Evaluation of Decellularized Bovine Tendon Sheets for Achilles Tendon Defect Reconstruction in a Rabbit Model

“…A number of studies showed that PRP could improve tendon cell proliferation and matrix synthesis in vitro, 52 augment tendon healing in vivo, 8 and improve cell-material interaction. 23 According to a previous study on rabbit PRP, 9 the mean number of platelets in whole blood was (259.06 ± 88.04) × 10 3 /µL, and that in PRP was (924.77 ± 330.86) × 10 3 /µL. In addition, the mean number of leukocytes in whole blood was (6.18 ± 2.01) × 10 3 /µL, and that in PRP was much decreased, with (0.06 ± 0.09) × 10 3 /µL.…”

Three dimensional nanofibrous and compressible poly(L-lactic acid) bone grafts loaded with platelet-rich plasma

“…It is known that treatment of PRP with CaCl 2 enables platelet activation leading to fibrin network formation [41]. First, activated and inactivated platelets were analyzed on coverslips since our grafts consist of fiber structure and to distinguish fibrin network can be difficult.…”