Comparison of three different phosphorescent methodologies in solution for the analysis of naphazoline in pharmaceutical preparations

Abstract

We present results from a comparative study of three proposed phosphorimetric methods for determination of naphazoline (NPZ) in solution. The first method is based on use of micelles to stabilize phosphorescence signals in solutions at room temperature (MS-RTP). The second is based on the use of a heavy atom salt and sodium sulfite as an oxygen scavenger to obtain room-temperature phosphorescence (HAI-RTP) in solution. The last method employs an optical sensor for NPZ based on the phosphorescent properties of the analyte on a solid sensor phase. The aim of this work was to compare time consumption, simplicity, sensitivity, selectivity, detection, and quantification limits for use of these three phosphorimetric methods to determine naphazoline in pharmaceutical preparations. The most simple, sensitive, and reproducible of the three methods for naphazoline analysis is the HAI-RTP method. Detection limits are 4.9, 1.7, and 9.4 ng mL(-1), respectively, for the MS-RTP, HAI-RTP, and optosensor methods.

Determination of naphazoline hydrochloride in biological and pharmaceutical samples by a quantum dot‐assisted chemiluminescence system using response‐surface methodology

“…Naphazoline, an imidazole derivative, belongs to sympathomimetic agents with a potent α‐adrenergic activity. Naphazoline hydrochloride (NH) is used in over‐the‐counter ocular and nasal drops as a vasoconstrictor and decongestant and has also proved to be a substance of antioxidant characteristics . Determination of NH is necessary in the quality control (QC) process during production of its medicinal drugs.…”

“…Various analytical methods have been proposed for determination of NH, namely spectrofluorimetry , phosphorimetry , spectrophotometry , electrochemistry , liquid chromatography , capillary electrophoresis (CE) , and CL . To the best of our knowledge, up to now only two CL methods have been reported for determination of NH which may be considered as preliminary attempts to approach the subject.…”

A validated stability-indicating HPLC method for the simultaneous determination of pheniramine maleate and naphazoline hydrochloride in pharmaceutical formulations

“…[7,8]. Naphazoline hydrochloride in pharmaceutical formulations or biological fluids either alone or in combination with other drugs was also detected by many methods, such as spectrophotometry [9,10], gas chromatography [11], high performance liquid chromatography [12-15], capillary electrophoresis [16-18], atomic emission and atomic absorption spectrometry [19], electrochemical method [20] and luminescence method [21,22]. However, in some extent, the above-described methods are limited in either low sensitivity or specificity.…”

Online naphazoline quality control by micellar‐enhanced spectrofluorimetry

“…Capillary electrophoresis , atomic absorption and emission and electrochemical methods have also been reported. Different luminescence methods have also been developed for NPZ determination, based on its intrinsic fluorescence and phosphorescence emission . However, most of the reported methods for NPZ determination present some practical complications for routine laboratory use: photometric methods are not selective and sensitive enough, separative methods are relatively expensive and time consuming and luminescence methods have been developed in a batch modality.…”