Comparison of Three Methods for Cyclosporine Therapeutic Monitoring

A comparison of liquid chromatography-tandem mass spectrometry (LC-MS/MS) and enzyme-multiplied immunoassay technique (EMIT) for the determination of the cyclosporin A concentration in whole blood from Chinese patients

“…This leads to severe infection and adverse reactions including nephro-, hepato-and neurotoxicity (4)(5)(6). Many commercially available assays are used to monitor CyA levels; these assays involve techniques such as an enzyme-multiplied immunoassay (EMIT), an enzyme-linked immunosorbent assay (ELISA), and a fluorescence polarization immunoassay (FPIA) (7), that utilize the same monoclonal antibody against CyA. However, these methods are not specific enough as they cannot distinguish CyA from its metabolites, which may increase in concentration during CyA treatment or during liver dysfunction.…”

Could salivary cyclosporine dosage replace the whole blood cyclosporine measurements in renal transplant patients?

“…The technique, however, is not practical in clinical settings. [142122] Alternatively, measurements of CsA level in the early polyclonal immunoassays, which are significanly easier, suffer from interference by metabolites present in unpredictable excess. [23] To resolve this, an effort was made to develop more specific and robust procedures for the measurements of CsA alone to give values similar to those obtained by HPLC.…”

“…In particular, the Axsym monoclonal fluorescent polarization immunoassay (FPIA) technique (Abbott Laboratories) has been demonstrated to compare favorably with HPLC for the measurement of blood CsA concentration, and may be a more accurate method for the assessment of CsA salivary concentration. [14]…”

“…Whole blood levels of CsA were determined on the same day using the monoclonal FPIA kit (TDx®/TDxFLx® Cyclosporin Monoclonal, Abbott Diagnostics ® , Wiesbaden, Germany) using a random and continuous access immunoassay analyzer. [11121314]…”

Quantitative LC Analysis of Cyclosporine A in Ocular Samples

“…Many papers have been published on the analysis of CyA in biological fluids [17][18][19][20]. Analytical methods such as HPLC [17,21,22], liquid chromatography-mass spectrometry [8], capillary electrophoresis [18], and immunoassay [19,23] have been reported for this purpose. Sample preparation and extraction is usually needed in analytical methods [17,21].…”