Comparison of Three Ternary Lipid Bilayer Mixtures: FRET and ESR Reveal Nanodomains

Heberle, Frederick A.; Wu, Jing; Goh, Shih Lin; Petruzielo, Robin S.; Feigenson, Gerald W.

doi:10.1016/j.bpj.2010.09.064

Cited by 202 publications

(313 citation statements)

References 37 publications

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“…There are many reports on the characterization of the phase-diagram and micro-domain structures on a liposome membrane [15][16][17][18]. Fluorescence microscopy and fluorescence resonance energy transfer (FRET) assays have been developed to detect domains in living membranes.…”

Section: Methods For Characterizing Micro-/nano-domain Structure On LImentioning

confidence: 99%

Use Liposome as a Designable Platform for Molecular Recognition ~ from “Statistical Separation” to “Recognitive Separation” ~

Umakoshi

Suga

2013

SERDJ

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A liposome membrane system attracts much interest for its use as a "designable" interface for the recognition of various biomolecules because it can provide a hydrophobic nano-environment in aqueous solution and can also induce a dynamic nature in response to the variation of its environment. The methods to characterize the membrane properties of liposome have been reviewed, focusing on the micro-phase separation of different lipids on the membrane and, also, nano-domain formation there.A possible design of the liposome membrane for the recognition of biomacromolecules was discussed based on the characterized properties, by employing the RNA molecule as a case study. Finally, a possible extension of the above-described strategy toward "recognitive separation" is discussed as a future possibility.

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Section: Methods For Characterizing Micro-/nano-domain Structure On LImentioning

confidence: 99%

Use Liposome as a Designable Platform for Molecular Recognition ~ from “Statistical Separation” to “Recognitive Separation” ~

Umakoshi

Suga

2013

SERDJ

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“…Bilayers are phase-separated, the two coexisting phases are fluid, and the value of ρ changes only the material parameters describing the energetics of that phase. These assumptions are reasonable because the compositions under consideration are squarely within the liquidliquid coexistence region of both of the ρ = 0.00 and ρ = 1.00 faces of the phase diagram tetrahedron [3]. This simplifies the mathematical model, as we can drop terms in the energy functional that are not related to morphology (such as any term that depends only on the local composition).…”

Section: B Simulation Modelmentioning

confidence: 99%

“…Performing GUV imaging studies on the POPC-containing system reveals that the liquid-liquid coexistence region appears uniform, unlike the macroscopic phase domains seen with DOPCcontaining mixtures. However, FRET, ESR [3], and neutron scattering studies [4] show that liquid-liquid coexistence is present with POPC. This observation implies that phase separation occurs in the POPC-containing system on the nanometer scale, thus not resolvable by ordinary light microscopy.…”

Section: Introductionmentioning

confidence: 99%

Competition between line tension and curvature stabilizes modulated phase patterns on the surface of giant unilamellar vesicles: A simulation study

2013

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When prepared in the liquid-liquid coexistence region, the four component lipid system distearoyl-phosphatidylcholine (DSPC)/dioleoyl-phosphatidylcholine (DOPC)/palmitoyl,oleoylphosphatidylcholine (POPC)/Cholesterol with certain ratios of DOPC and POPC shows striking modulated phase patterns on the surface of giant unilamellar vesicles (GUVs). In this simulation study we show that the morphology of these patterns can be explained by the competition of line tension (which tends to favor large round domains) and curvature, as specified by the Helfrich energy functional. In this study we use a Monte-Carlo simulation on the surface of a GUV to determine the equilibrium shape and phase morphology. We find that the patterns arising from these competing interactions very closely approximate those observed, the patterned morphologies represent thermodynamically stable configurations, and that the geometric nature of these patterns is closely tied to the relative and absolute values of the model parameters.

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“…[1][2][3][4][5][6] These membrane systems are widely considered as model systems that mimic the composition of the general mammalian cell plasma membrane. Observations of the same macroscopic miscibility transitions in cell membrane blebs provide compelling confirmation that this is an inherent property of cell membrane lipids.…”

Section: Introductionmentioning

confidence: 99%

Live Cell Plasma Membranes Do Not Exhibit a Miscibility Phase Transition over a Wide Range of Temperatures

et al. 2015

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Lipid:cholesterol mixtures derived from cell membranes, as well as their synthetic reconstitutions, exhibit well defined miscibility phase transitions and critical phenomena near physiological temperatures. This suggests that lipid:cholesterol-mediated phase separation plays a role in the organization of live cell membranes. However, macroscopic lipid phase separation is not generally observed in cell membranes and the degree to which properties of isolated lipid mixtures are preserved in the cell membrane remain unknown. A fundamental property of phase transitions is that the variation of tagged particle diffusion with temperature exhibits an abrupt change as the system passes through the transition, even when the two phases are distributed in a nanometer-scale emulsion. We support this using a variety of Monte-Carlo and atomistic simulations on model lipid membrane systems. However, temperature dependent fluorescence correlation spectroscopy of labeled lipids and membrane-anchored proteins in live cell membranes show a consistently smooth increase of the diffusion coefficient as a function of temperature. We find no evidence of a discrete miscibility phase transition throughout a wide range of temperatures: 14 -37 °C. This contrasts the behavior of giant plasma membrane vesicles (GPMVs) blebbed from the same cells, which do exhibit phase transitions and macroscopic phase separation. Fluorescence lifetime analysis of a DiI probe in both cases reveals a significant environmental difference between the live cell and the GPMV. Taken together, these data suggest the live cell membrane may avoid the miscibility phase transition inherent to its lipid constituents by actively regulating physical paramters, such as tension, in the membrane.

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Comparison of Three Ternary Lipid Bilayer Mixtures: FRET and ESR Reveal Nanodomains

Cited by 202 publications

References 37 publications

Use Liposome as a Designable Platform for Molecular Recognition ~ from “Statistical Separation” to “Recognitive Separation” ~

Use Liposome as a Designable Platform for Molecular Recognition ~ from “Statistical Separation” to “Recognitive Separation” ~

Competition between line tension and curvature stabilizes modulated phase patterns on the surface of giant unilamellar vesicles: A simulation study

Live Cell Plasma Membranes Do Not Exhibit a Miscibility Phase Transition over a Wide Range of Temperatures

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