Comparison of triterpene compounds of four botanical parts from Poria cocos (Schw.) wolf using simultaneous qualitative and quantitative method and metabolomics approach

Zhang, Guanhua; Wang, Hongxia; Xie, Wenyu; Wang, Qi; Wang, Xu; Wang, Chunying; Du, Yingfeng; Huo, Changhong; Wang, Qiao

doi:10.1016/j.foodres.2018.12.036

Cited by 36 publications

(16 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The 1 H- and 13 C-NMR spectra indicated that H-11 (δ 5.37)/C-11 (δ 116.5), H-7 (δ 5.61)/C-7 (δ 121.0), C-9 (δ 146.4), C-8 (δ 142.7), H-18 (δ 1.01), H-19 (δ 1.07), H-26 (δ 1.67), H-27 (δ 1.62), H-28 (δ 1.22), H-29 (δ 1.13), H-30 (δ 1.06), H-3 (δ 3.46), H-24 (δ 5.33), C-3 (δ 77.9), C-21 (δ 178.9), C-24 (δ 124.8), C-25 (δ 131.5). Based on the structural features of triterpene compounds and the data given in the literature, compound- 4 was identified as dehydrotrametenolic acid [ 1 , 2 , 19 ]. Dehydrotrametenolic acid belongs to the lanosta-7,9(11)-diene type triterpenes [ 4 ].…”

Section: Resultsmentioning

confidence: 99%

“…Additionally, P. cocos is used as a dietary supplement and in foods such as soups, dishes, snacks, and desserts for its health-promoting benefits. It can be divided into four parts, namely, Poriae Cutis (the epidermis, “fulingpi” in Chinese), Rubra Poria (the pink part near the epidermis), White Poria (the middle part, “baifuling” in Chinese), and Poria cum Radix Pini (the middle-plus-inner part, “fushen” in Chinese) [ 2 ]. Different parts of Poria cocos have different pharmacological effects and clinical applications.…”

Section: Introductionmentioning

confidence: 99%

“…Triterpenes isolated from P. cocos can be divided into four subgroups based on their chemical structure: lanosta-8-ene type, lanosta-7,9(11)-diene type, 3,4-seco-lanostan-8-ene type, and 3,4-seco-lanostan-7,9(11)-diene type compounds [ 4 ]. In prior research, 59 types of triterpenoids were separated from P. cocos ; 41 types were isolated from Poriae Cutis [ 2 , 4 ]. The total content of triterpenes and bioactive triterpenes in Poriae Cutis was found to be higher than in the sclerotia of P. cocos [ 1 , 5 , 6 , 7 ], and triterpenoids from the sclerotia of P. cocos showed anti-inflammatory activity [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Anti-Inflammatory Activity of Four Triterpenoids Isolated from Poriae Cutis

Zhang

Yin

Feng

et al. 2021

Foods

View full text Add to dashboard Cite

In this study, triterpenoid compounds from Poriae Cutis were separated by high-speed countercurrent chromatography (HSCCC) and identified using ultra-high performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry (UHPLC-QTOF-MS/MS) and nuclear magnetic resonance (NMR). The in vitro anti-inflammatory activities of the purified triterpenoids on RAW 264.7 cells were also investigated. Triterpenoids, poricoic acid B, poricoic acid A, dehydrotrametenolic acid, and dehydroeburicoic acid were obtained; their levels of purity were 90%, 92%, 93%, and 96%, respectively. The results indicated that poricoic acid B had higher anti-inflammatory activity than those of poricoic acid A by inhibiting the generation of NO in lipopolysaccharide (LPS)-induced RAW 264.7 cells. However, dehydrotrametenolic acid and dehydroeburicoic acid had no anti-inflammatory activity. In addition, the production of cytokines (TNF-α, IL-1β, and IL-6) in cells treated with poricoic acid B decreased in a dose-dependent manner in the concentration range from 10 to 40 μg/mL. The results provide evidence for the use of Poriae Cutis as a natural anti-inflammatory agent in medicines and functional foods.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Anti-Inflammatory Activity of Four Triterpenoids Isolated from Poriae Cutis

Zhang

Yin

Feng

et al. 2021

Foods

View full text Add to dashboard Cite

show abstract

“…The data determined in this study not only lend sufficient support to the above literature but also determine the influence of the growth period on the triterpenoid acid content in four parts of Fushen. However, some studies have found that dehydropachymic acid and dehydrotumulosic acid contents are higher in the fulingpi (Zhang et al, 2019 ). This could be caused by different harvest times, according to the experimental results.…”

Section: Discussionmentioning

confidence: 99%

Untargeted Metabolomics and Targeted Quantitative Analysis of Temporal and Spatial Variations in Specialized Metabolites Accumulation in Poria cocos (Schw.) Wolf (Fushen)

et al. 2021

View full text Add to dashboard Cite

Poria cocos (Schw.) Wolf is a saprophytic fungus that grows around the roots of old, dead pine trees. Fushen, derived from the sclerotium of P. cocos but also containing a young host pine root, has been widely used as a medicine and food in China, Japan, Korea, Southeast Asian countries, and some European countries. However, the compound variations at the different growth periods and in the different parts of Fushen have not previously been investigated. In this study, an untargeted metabolomics approach based on ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q/TOF-MS) and targeted quantitative analysis was utilized to characterize the temporal and spatial variations in the accumulation of specialized metabolites in Fushen. There were 119 specialized metabolites tentatively identified using the UPLC-Q/TOF-MS. The nine growth periods of Fushen were divided into four groups using partial least squares discrimination analysis (PLS-DA). Four different parts of the Fushen [fulingpi (FP), the outside of baifuling (BO), the inside of baifuling (BI), and fushenmu (FM)] were clearly discriminated using a PLS-DA and orthogonal partial least squares discrimination analysis (OPLS-DA). Markers for the different growth periods and parts of Fushen were also screened. In addition, the quantitative method was successfully applied to simultaneously determine 13 major triterpenoid acids in the nine growth periods and four parts. The quantitative results indicated that the samples in January, March, and April, i.e., the late growth period, had the highest content levels for the 13 triterpenoid acids. The pachymic acid, dehydropachymic acid, and dehydrotumulosic acid contents in the FM were higher than those in other three parts in March, whereas the poricoic acid B, poricoic acid A, polyporenic acid C, dehydrotratrametenolic acid, dehydroeburicoic acid, and eburicoic acid in FP were higher beginning in October. These findings reveal characteristics in temporal and spatial distribution of specialized metabolites in Fushen and provide guidance for the identification of harvesting times and for further quality evaluations.

show abstract

“…For triterpenoids, the cleavage at the glycosidic bonds was the fragmentation pattern and produced representative fragments with the loss of glucose (Glc, 162 Da), rhamnose (Rha, 146 Da), xylose (Xyl, 132 Da), and/or arabinose (Ara, 132 Da). However, these substances were prone to lose H Based on the cleavage rule of reference standards and information from related literature [2,[30][31][32][33][34][35][36], the triterpenoids could eliminate the neutral fragments such as Glc (162 Da), Rha (146 Da), Xyl (132 Da), Ara (132 Da), GlcA (176 Da), H 2 O (18 Da), CO2 (44 Da), HCOOH (46 Da), and/or the combination of these fragments. The characterization of triterpenoids in YWD was concluded by referring to these fragmentation mechanisms, and tentatively identified in YWD were a total of 59 triterpenoids, including 22 ginsenosides (labeled as P87, P89, P94-P96, P115, P122, P127-P128, P131, P136, P139, P145, P149-P151, P156, P182, P188, P192-P193, P208), 22 triterpene saponins (labeled as P181, P187, P203, P206, P209, P212-P213, P215, P217-P222, P224) and 15 triterpene acids (labeled as P103, P107-P110, P114, P116, P119, P135, P137, P140-P141, P146-P147, P152, P157-P158, P163, P170, P172, P180, P184) (Table S3).…”

Section: Characterization Of Triterpenoids In Ywdmentioning

confidence: 99%

Comprehensive characterization and identification of chemical constituents in Yangwei decoction using ultra high performance liquid chromatography coupled with electrospray ionization quadrupole time‐of‐flight tandem mass spectrometry

Fan

Tian

et al. 2022

J of Separation Science

View full text Add to dashboard Cite

Yangwei decoction, a classical traditional Chinese medicine prescription, has been widely used to treat exogenous cold and internal injury with damp stagnation for many centuries. However, its systematic chemical profiling remains ambiguous, which has hampered the interpretation of pharmacology and the mechanism of its formula. In the present study, ultra‐performance liquid chromatography coupled with electrospray ionization quadrupole time‐of‐flight tandem mass spectrometry method was successfully established for the first time to separate and identify the complicated components of Yangwei decoction. The accurate mass data of the protonated molecules, deprotonated molecules, and fragment ions were detected in positive and negative ion modes. A total of 226 compounds in Yangwei decoction were tentatively identified and unambiguously characterized by comparing their retention times and mass spectrometry data with those of reference standards and literature, including 24 lignans, 18 alkaloids, 9 phenylpropanoid glycosides, 76 flavonoids, 59 triterpenoids, 17 organic acids, 7 gingerols, 8 lactones, and 8 other compounds. The present study provides a novel method of constituents characterization for well‐known Chinese medicine prescriptions. The study aims to lay a robust foundation for future research, providing the holistic quality control and pharmacology of Yangwei decoction.

show abstract

Comparison of triterpene compounds of four botanical parts from Poria cocos (Schw.) wolf using simultaneous qualitative and quantitative method and metabolomics approach

Cited by 36 publications

References 16 publications

Anti-Inflammatory Activity of Four Triterpenoids Isolated from Poriae Cutis

Anti-Inflammatory Activity of Four Triterpenoids Isolated from Poriae Cutis

Untargeted Metabolomics and Targeted Quantitative Analysis of Temporal and Spatial Variations in Specialized Metabolites Accumulation in Poria cocos (Schw.) Wolf (Fushen)

Comprehensive characterization and identification of chemical constituents in Yangwei decoction using ultra high performance liquid chromatography coupled with electrospray ionization quadrupole time‐of‐flight tandem mass spectrometry

Contact Info

Product

Resources

About