Comparison of twelve human milk oligosaccharides in mature milk from different areas in China in the Chinese Human Milk Project (CHMP) study

Zhang, Wenyuan; Vervoort, Jacques; Pan, Jiancun; Gao, Peng; Zhu, Huiquan; Wang, Xiaodan; Zhang, Yumeng; Chen, Baorong; Liu, Ying; Li, Yuanyuan; Pang, Xiaoyang; Zhang, Shuwen; Jiang, Shilong; Lü, Jing; Lyu, Jiaping

doi:10.1016/j.foodchem.2022.133554

Cited by 17 publications

(14 citation statements)

References 56 publications

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“…The difference can be easily understood because the content of oligosaccharides in HM is dynamic due to diverse individual factors, such as genotype, diet, age, and region. 11 In contrast, infant formula is sourced from blended raw milk and undergoes a standardized industrial process, ensuring a stable nutritional composition. Therefore, infant formula supplemented with extra oligosaccharides can provide an adequate and comprehensive composition for infants when breast milk contains insufficient oligosaccharides due to individual variations.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Evaluation of Prebiotic Glycan Composition in Human Milk and Infant Formula: Profile of Galacto-Oligosaccharides and Absolute Quantification of Major Milk Oligosaccharides by UPLC-Cyclic IM-MS and UPLC-MS/MS

Hou,

Wang,

Yang

et al. 2024

J. Agric. Food Chem.

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Prebiotic oligosaccharides have attracted immense interest in the infant formula (IF) industry due to their unique health benefits for infants. There is a need for the reasonable supplementation of prebiotics in premium IF products. Herein, we characterized the profile of galacto-oligosaccharides (GOS) in human milk (HM) and IF using ultrahigh-performance liquid chromatography−cyclic ion mobility−mass spectrometry (UPLC-cIM-MS) technique. Additionally, we further performed a targeted quantitative analysis of five essential HM oligosaccharides (HMOs) in HM (n = 196), IF (n = 50), and raw milk of IF (n = 10) by the high-sensitivity UPLC-MS/MS method. HM exhibited a more abundant and variable HMO composition (1183.19 to 2892.91 mg/L) than IF (32.91 to 56.31 mg/L), whereas IF contained extra GOS species and non-negligible endogenous 3′-sialyllactose. This also facilitated the discovery of secretor features within the Chinese population. Our study illustrated the real disparity in the prebiotic glycome between HM and IF and provided crucial reference for formula improvement.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Evaluation of Prebiotic Glycan Composition in Human Milk and Infant Formula: Profile of Galacto-Oligosaccharides and Absolute Quantification of Major Milk Oligosaccharides by UPLC-Cyclic IM-MS and UPLC-MS/MS

Hou,

Wang,

Yang

et al. 2024

J. Agric. Food Chem.

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“…PC11 (mainly vitamin A and vitamin C) and PC12 (vitamin B3) emerged as the positive predictive factor for 3-FL. A most recent study also pointed out that vitamin intake was associated with HMOs levels [ 20 ]. Our results, in accordance with the previous study, both suggest that vitamin supplements might be a positive nutrition factor to enhance milk HMO concentration in lactating mothers.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, other benefits of eating more vegetables may also contribute to enhanced HMO content of breastmilk. In 2019, The Lancet Commission emphasized the broad benefits of eating vegetables [ 20 ]. A recent study also found that higher vegetable intake in mothers is associated with a lower incidence of allergy in offspring [ 21 ].…”

Section: Discussionmentioning

confidence: 99%

Vitamins, Vegetables and Metal Elements Are Positively Associated with Breast Milk Oligosaccharide Composition among Mothers in Tianjin, China

Mao

Liu

et al. 2022

Nutrients

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Background: Human milk oligosaccharides (HMOs) are a group of breast milk carbohydrates exerting pivotal benefits for breastfed infants. Whether maternal diet is associated with breastmilk HMO composition has not been well-characterized. Objectives: We investigated the associations between dietary nutrient intake and HMO concentrations in a general pregnant and postpartum population. Methods: A total of 383 breast milk samples and the corresponding food frequency questionnaires during 0–400 postpartum days from 277 mothers were collected. Six different HMOs were detected in mothers’ milk. The correlation between nutrients and HMOs were analyzed using a linear mixed-effects model. Results: We found plant nutrients, vitamin A, vitamin C and vegetables as positive predictors of 3-fucosyllactose; vitamin B1 and vitamin B2 were positive predictors for 2′-fucosyllactose level and the sum of 2′-fucosyllactose and 3-fucosyllactose; tocopherol and metal elements were positive predictors for 3′-sialyllactose; and metal elements were positively associated with the sum of all the six HMOs; the milk and lactose intake was a positive predictor of lacto-N-tetraose levels and the sum of lacto-N-tetraose and lacto-N-neotetraose. Conclusions: The results show that vegetables, vitamins and metal elements are dietary components positively associated with HMO concentrations.

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“…Other studies also showed that the profile of HMOs were influenced by geographic, environmental, maternal anthropometric, and dietary factors (McGuire et al, 2017; Wu et al, 2020). For example, the concentration of lacto‐ N ‐neohexaose was negatively correlated with latitude and the concentrations of 3′‐SL were positively correlated with latitude (Zhang et al, 2022) and Davis et al (2017) studied HMOs in 33 Gambian women and showed that mothers produce more HMOs during the dry season. Another study found that the concentrations of lacto‐ N ‐fucopentaose III was relatively low, whereas the concentration of disialyllacto‐ N ‐tetraose was relatively high in spring (Azad et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Many studies have shown that breastfed babies have a healthier gut and richer intestinal flora than those fed with infant formula, due to the higher concentration and diversity of oligosaccharides in breast milk (Azad et al, 2018; Davis et al, 2017; Hester et al, 2013; Hobbs et al, 2021; Jacobi et al, 2016; Zhang et al, 2022). Infant formula has undergone significant modifications over the last few decades with the addition of bioactive ingredients such as prebiotics, lactoferrin, and milk fat globular membranes.…”

Section: Introductionmentioning

confidence: 99%

Differences in the content of human milk oligosaccharides between foremilk and hind milk

Sun

Liu

et al. 2023

Food Bioengineering

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Human milk oligosaccharides (HMOs) are the second largest carbohydrate component and the third largest nutrient after lactose, and their content and composition are related to the genetic characteristics of the mother, lactation period, geography, and other factors. This study examined the differences in fat content, protein composition, and oligosaccharides content between foremilk and hind milk in human milk. The results showed that the fat content of human milk was significantly higher in the hind milk than in the foremilk. However, no significant differences in the composition and content of protein in the fore and hind milk. With respect to oligosaccharides, the total amount of HMOs (based on seven oligosaccharides, lacto-N-neotetraose (LNnT), lacto-N-tetraose (LNT), 2′-fucosyllactose (2′-FL), 3′-fucosyllactose (3′-FL), 3′-galactosyllactose (3′-GL), 3′-sialyllactose (3′-SL), and 6′-sialyllactose (6′-SL) in the hind milk was always higher than in the foremilk in almost all mothers. Similar trend was found in specific oligosaccharide including LNnT, LNT, 2′-FL, 3′-FL, and 3′-GL. On the other hand, sialylated oligosaccharides, 3′-SL, and 6′-SL were quite consistent in foremilk and hind milk.

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Comparison of twelve human milk oligosaccharides in mature milk from different areas in China in the Chinese Human Milk Project (CHMP) study

Cited by 17 publications

References 56 publications

Evaluation of Prebiotic Glycan Composition in Human Milk and Infant Formula: Profile of Galacto-Oligosaccharides and Absolute Quantification of Major Milk Oligosaccharides by UPLC-Cyclic IM-MS and UPLC-MS/MS

Evaluation of Prebiotic Glycan Composition in Human Milk and Infant Formula: Profile of Galacto-Oligosaccharides and Absolute Quantification of Major Milk Oligosaccharides by UPLC-Cyclic IM-MS and UPLC-MS/MS

Vitamins, Vegetables and Metal Elements Are Positively Associated with Breast Milk Oligosaccharide Composition among Mothers in Tianjin, China

Differences in the content of human milk oligosaccharides between foremilk and hind milk

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