The aim of this study was to assess the exposure to organic dust and (1 ? 3)-b-D-glucans in the buildings where an intensive breeding of swine is going on and evaluation of the impact of the breeding technical conditions on the observed levels of bioaerosols. The study was carried out in 30 swine farms differentiated by the size of the herd and technical conditions of breeding. In 35 randomly selected buildings, air samples were collected by stationary measurements to determine the concentrations of organic dust and (1 ? 3)-b-D-glucans in inhalable and respirable fractions. Furthermore, each of the investigated buildings was precisely characterized by means of a questionnaire for technical conditions and type of breeding. In each of the points, the microclimate parameters were measured, i.e., temperature, relative humidity, CO 2 concentration and air velocity. The analyzed levels of organic dust and (1 ? 3)-b-Dglucans were characterized by a wide range of concentrations. For inhalable fraction, they reached respectively: organic dust (0.43-11.8 mg/m 3 ), (1 ? 3)-b-D-glucans (14-3,594 ng/m 3 ). For respirable fraction, the results were as follows: organic dust (0.01-4.69 mg/ m 3 ), (1 ? 3)-b-D-glucans (1-703 ng/m 3 ). The concentrations of (1 ? 3)-b-D-glucans were positively correlated with organic dust (r = 0.68; p \ 0.001). The most significant factor increasing the concentrations of organic dust and (1 ? 3)-b-D-glucans was the use of bedding in the form of cut straw. Additionally, the levels of (1 ? 3)-b-D-glucans were affected by manual forage feeding, mechanical manure disposal and the lack of the liquid manure container in breeding buildings. In view of the hazardous effects of biological agents on the health of swine-breeding workers, the swine management systems without beddings should be used, along with automated dosing techniques.