Comparison of two different methods in the cryopreservation of Eurasian perch (Perca fluviatilis) sperm

Bernáth, Gergely; Bokor, Zoltán; Kása, Eszter; Várkonyi, Levente; Hegyi, Árpád; Kollár, Tímea; Urbányi, Béla; Żarski, Daniel; Ifj., J. Radóczi; Horváth, Ákos

doi:10.1016/j.cryobiol.2014.12.003

Cited by 27 publications

(9 citation statements)

References 10 publications

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“…CASA development Acipenser ruthenus sterlet (Boryshpolets et al, 2013) Anguilla anguilla European eel (Gallego et al, 2013b) Cyprinus carpio common carp (Boryshpolets et al, 2013) Danio rerio zebrafish (Wilson-Leedy et al, 2009) Oncorhynchus mykiss rainbow trout (Boryshpolets et al, 2013) Sperm cryopreservation Acipenser baerii Siberian sturgeon (Judycka et al, 2015;Sieczynski et al, 2015) Anguilla anguilla European eel (Asturiano et al, 2004(Asturiano et al, , 2007 Brycon orbignyanus piracanjuba (López et al, 2015) Clarias gariepinus African catfish (Rurangwa et al, 2001) Cyprinus carpio common carp (Warnecke & Pluta, 2003) Dicentrarchus labrax European seabass (Cabrita et al, 2011) Gadus morhua Atlantic cod (Butts et al, 2011a) Perca fluviatilis Eurasian perch (Bernáth et al, 2015) Salmo salar Atlantic salmon (Dziewulska et al, 2011) Sparus aurata gilthead seabream (Beirão et al, 2011b;Cabrita et al, 2010) Thymallus thymallus grayling (Horváth et al, 2015) Sperm chilled storage Anguilla anguilla European eel (Peñaranda et al, 2010c) Hippoglossus hippoglossus Atlantic halibut (Babiak et al, 2006) Oncorhynchus mykiss rainbow trout (Lahnsteiner et al, 2004) Salmo trutta brown trout (Formicki et al, 2015) Steindachneridion parahybae surubim-do-Paraíba (Sanches et al, 2015) Activation media tests Coregonus lavaretus European whitefish (Dziewulska et al, 2015) Cyprinus carpio common carp (Zarski et al, 2015) Oncorhynchus mykiss steelhead (Kanuga et al, 2012) Perca fluviatilis Eurasian perch (Lahnsteiner, 2011) Broodstock management Dicentrarchus labrax European seabass (Felip et al, 2006) Solea senegalensis Senegalese sole (Beirão et al, 2011a) Spermiation induction Acipenser ruthenus sterlet (Alavi et al, 2012) Anguilla anguilla European eel ...…”

Section: Speciesmentioning

confidence: 99%

Hormonal manipulations for the enhancement of sperm production in cultured fish and evaluation of sperm quality

2017

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This article reviews the use of hormonal treatments to enhance sperm production in aquaculture fish and the methods available for evaluating sperm quality. The different types of testis development are examined and a brief review is presented of the endocrine regulation of spermatogenesis in fishes, including the increasing evidence of the existence of spermatozoa subpopulations. Hormonal manipulations are employed to induce spermatogenesis in species such as the freshwater eels, to synchronize maximal sperm volume to ovulation for in vitro fertilization and to enhance sperm production in species with poor spermiation. The hormones that are employed include gonadotropins (GtH) of piscine or mammalian origin, and gonadotropin-releasing hormone agonists (GnRHa) administered by injections or controlled-release delivery systems, with or without dopaminergic inhibitors. Pheromones in the culture water and hormones added to the sperm in vitro have also been employed to enhance spermiation and sperm quality, respectively, in some fishes. Hormonal therapies usually do not affect sperm quality parameters, except in cases where fish fail to spermiate naturally or produce very small volumes of high-density sperm. Different parameters have been used to evaluate fish sperm quality, including sperm volume and density, spermatozoa motility and morphometry, and seminal plasma composition. The development of Computer-Assisted Sperm Analysis (CASA) systems made possible the estimation of a higher number of sperm motion parameters using an objective, sensitive and accurate technique. The development of Assisted Sperm Morphology Analysis (ASMA) software has introduced a new approach for sperm evaluation studies, demonstrating changes in the spermatozoa related to reproductive season, hormonal treatments or the cryopreservation processes, and how these may be related to changes in sperm motility and fertilization capacity. The article concludes with a few practical protocols for the enhancement of sperm production in aquaculture species.

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Section: Speciesmentioning

confidence: 99%

Hormonal manipulations for the enhancement of sperm production in cultured fish and evaluation of sperm quality

2017

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“…Although the progressive motility (47% ± 5%) was high following thawing but the fertilization rate (32% ± 6%) was negatively affected by an agglutination phenomenon of sperm (Bernáth et al, ). Controlled‐rate freezer can ensure a stable cooling environment during the freezing process which can increase the sperm quality following thawing (Bernáth et al, ). According to our hypothesis and experience, using large straws or vials for carp sperm cryopreservation can be more adaptable in the regular hatchery practice in order not to waste time with the thawing of numerous straws.…”

Section: Introductionmentioning

confidence: 99%

The comparison of two different extenders for the improvement of large‐scale sperm cryopreservation in common carp (Cyprinus carpio)

Várkonyi

Bokor

Molnár

et al. 2018

Reprod Domestic Animals

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Contents In our study, a traditionally used (Grayling, already used in cyprinid species) and a newly tested (Pike) extender was tested to avoid sperm agglutination phenomenon following thawing during carp sperm cryopreservation. A large‐scale (elevated volume of sperm) freezing method in a controlled‐rate freezer using 5 ml straw and 10 ml cryotube was also systematically established. In all experiments, the sperm cryopreserved in using Grayling extender (except only one sample) showed an agglutination phenomenon (damaged and intact cells adhered to each other) after thawing where Pike extender resulted the regular cell suspension. No significant difference was observed between the two cryopreserved groups (Pike and Grayling extender) in all motility parameters using the 0.5 ml straw and the polystyrene box. Similarly, motility parameters did not show a significant difference in the two frozen groups with the 5 ml straw, also in the polystyrene box. A significantly higher progressive motility (pMOT, Grayling: 54% ± 8%, Pike: 37% ± 5%), straight line velocity (VSL, Grayling: 50 ± 5 µm/s, Pike: 39 ± 4 µm/s) and beat cross frequency (BCF, Grayling: 20 ± 1 Hz, Pike: 17 ± 1 Hz) was observed in the case of the grayling extender by the 5 ml straw cryopreserved in a controlled‐rate freezer (CRF) compare to the pike extender. A significantly higher VSL (Grayling: 45 ± 3 µm/s, Pike: 38 ± 4 µm/s) was observed by the grayling extender using the 10 ml cryotube than with the pike extender. Despite the randomly occurring differences in a few parameters, our new controlled freezing method using the newly tested Pike extender, the 5 ml straw or the 10 ml cryotube can be a good solution for the preservation of elevated volume of carp sperm.

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“…1) but there is an increasing interest in designing cryopreservation protocols for species from tropical and subtropical areas (Maria et al, 2015; Viveiros and Godinho, 2009). Traditional empirical studies in a factorial experimental design testing a range of freezing rates, cryoprotectants or extenders are still conducted in order to develop cryopreservation protocols for species such as Atlantic salmon (Dziewulska et al, 2011), beluga sturgeon (Aramli et al, 2015), Siberian sturgeon (Judycka et al, 2015), perch (Bernáth et al, 2015) or tambaqui (Maria et al, 2015; Varela Junior et al, 2015). Nevertheless, the evaluation of sperm quality after freezing/thawing has evolved, allowing a better understanding of the mechanisms linking to the compromised quality during the cryopreservation process.…”

Section: Fish Sperm Cryopreservationmentioning

confidence: 99%

Cryobanking of aquatic species

et al. 2017

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This review is focused on the applications of genome cryobanking of aquatic species including freshwater and marine fish, as well as invertebrates. It also reviews the latest advances in cryobanking of model species, widely used by the scientific community worldwide, because of their applications in several fields. The state of the art of cryopreservation of different cellular types (sperm, oocytes, embryos, somatic cells and primordial germ cells or early spermatogonia) is discussed focusing on the advantages and disadvantages of each procedure according to different applications. A special review on the need of standardization of protocols has also been carried out. In summary, this comprehensive review provides information on the practical details of applications of genome cryobanking in a range of aquatic species worldwide, including the cryobanks established in Europe, USA, Brazil, Australia and New Zealand, the species and type of cells that constitute these banks and the utilization of the samples preserved. Statement of relevance This review compiles the last advances on germplasm cryobanking of freshwater and marine fish species and invertebrates, with high value for commercial aquaculture or conservation. It is reviewed the most promising cryopreservation protocols for different cell types, embryos and larvae that could be applied in programs for genetic improvement, broodstock management or conservation of stocks to guarantee culture production.

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Comparison of two different methods in the cryopreservation of Eurasian perch (Perca fluviatilis) sperm

Cited by 27 publications

References 10 publications

Hormonal manipulations for the enhancement of sperm production in cultured fish and evaluation of sperm quality

Hormonal manipulations for the enhancement of sperm production in cultured fish and evaluation of sperm quality

The comparison of two different extenders for the improvement of large‐scale sperm cryopreservation in common carp (Cyprinus carpio)

Cryobanking of aquatic species

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