Comparison of two observational methods, scanning electron and confocal laser scanning microscopies, in the adhesive interface analysis of endodontic sealers to root dentine

Tedesco, Maybell; Chain, Marcelo Carvalho; Bortoluzzi, Eduardo Antunes; Garcia, Lucas da Fonseca Roberti; Alves, Ana Maria Hecke; Teixeira, Cleonice da Silveira

doi:10.1007/s00784-018-2336-y

Cited by 34 publications

(43 citation statements)

References 30 publications

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“…The presence of cohesive failure in AH Plus group can be explained by the higher bond strength of the sealer to dentin, and the deeper sealer penetration into the dentinal tubules (14). Conversely, the adhesive failure in most of the specimens from the Endofill group might be explained by the low bond strength of zinc-oxide based sealers to root dentin (4,9,16).…”

Section: Discussionmentioning

confidence: 99%

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Correlation between Bond Strength to Dentin and Sealers Penetration by Push-Out Test and CLSM Analysis

et al. 2019

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This study correlated the bond strength (BS) and dentin penetration of different sealers by push-out test and Confocal Laser Scanning Microscopy (CLSM) analysis. Forty-five root canals were prepared according to the crown-down technique and filled with gutta-percha associated to the following sealers (n=15): Endofill, AH Plus and MTA Fillapex. Five canals of each group were filled with the sealers added with 0.1% Rhodamine B dye. Next, the specimens were transversely sectioned and submitted to the push-out test (n=10) and CLSM analysis (n=5). The BS data showed the following means (MPa) and standard deviation: AH Plus (4.17±1.86); MTA Fillapex (3.13±1.96) and Endofill (2.10±1.03). Statistical analysis (two-way ANOVA, α=0.05) showed significant difference among sealers (p<0.001) and root canal thirds (p<0.001). The BS results of Endofill and MTA Fillapex were statistically similar (p>0.05), however, they were statistically different from AH Plus (p<0.001). The regional analysis of BS showed similarity between middle and apical thirds (p>0.05), and both were different from coronal portion (p<0.001). CLSM analysis verified tags formation in all groups and higher penetration of the specimens filled with AH Plus (p<0.05). The Kendall test (correlation between BS to dentin and sealer penetration into dentinal tubules) and the Pearson test (between failures pattern and sealer penetration into dentinal tubules) did not show correlation between the variables evaluated for all the tested sealers (p>0.05). AH Plus group had higher BS to dentin, and deeper tags formation than the other sealers. There was no significant correlation between BS and intratubular penetration of the tested sealers.

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Section: Discussionmentioning

confidence: 99%

“…Thirty slices per group were tested for bond strength. The slices obtained from the other five roots, which were filled incorporating the rhodamine B, were kept in an environment protected from the light and moisture, until analysis under CLSM (9).…”

Section: Push-out Testmentioning

confidence: 99%

Correlation between Bond Strength to Dentin and Sealers Penetration by Push-Out Test and CLSM Analysis

et al. 2019

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“…37 Confocal laser scanning microscope (CLSM) has been employed for evaluating the sealer penetration. [38][39][40] CLSM has several advantages, such as being able to use non-decalcified or hard tissue samples that do not require a specific sectioning technique (sputter coating), providing detailed information about the presence and distribution of sealers at relatively low magnification through the use of fluorescent rhodamine-marked sealers, and allowing the exclusion of artifacts from the sample. 38 In the present study, sealer penetration into root dentin was influenced by the irrigant used.…”

Section: Discussionmentioning

confidence: 99%

Effect of peracetic acid used as single irrigant on the smear layer, adhesion, and penetrability of AH Plus

et al. 2019

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The aim of this study was to evaluate the effect of peracetic acid (PAA) as a single irrigant on the smear layer, on the intraradicular dentinal bond strength, and on the penetrability of an epoxy-based resin sealer into the dentinal tubules. A total of 120 roots were distributed into 4 groups according to the irrigant used in root canal preparation: 1% PAA (PAA); 2.5% NaOCl followed by final irrigation with 17% EDTA and 2.5% NaOCl (NaOCl-EDTA-NaOCl); 2.5% NaOCl (NaOCl); and saline solution (SS). The smear layer was evaluated using scanning electron microscopy. The bond strength of an epoxy-based resin sealer (AH Plus) to root dentin was evaluated by the push-out test and penetrability of the sealer into dentinal tubules was observed by confocal laser microscopy. The results were analyzed by the Kruskal-Wallis and the Dunn post-test (α = 0.05). The use of 1% PAA as single root canal irrigant provided smear layer removal and improved the penetrability and bond strength of AH Plus to root dentin in a manner similar to that of the NaOCl-EDTA-NaOCl group (p > 0.05). The NaOCl and SS groups had higher values of smear layer and lower values of sealer penetrability and dentin bond strength than the PAA and NaOCl-EDTA-NaOCl groups (p < 0.05). Thus, 1% PAA has the potential to be used as a single irrigant in root canals.

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“…9,10 For this reason, different materials, 11,12 techniques, 13,14 and modifications 15,16 have been proposed and studied using several methodologies. 17,18 One of the preferred tools to assess the adhesive interface and the topographic features of the root canal filling is confocal laser scanning microscopy (CLSM). It stands out because it allows samples to be measured in depth despite a wet environment.…”

Section: Introductionmentioning

confidence: 99%

“…CLSM is capable of accurately determining the degree of adaptation and penetration of the root canal filling materials. 18 This in vitro study aimed at determining PGFA, PSFA, and PVFA in oval-shaped root canals obturated with CLCT and CWCT and assessing two respective procedural modifications using CLSM.…”

Section: Introductionmentioning

confidence: 99%

Percentage of Gutta-Percha-, Sealer-, and Void-Filled Areas in Oval-Shaped Root Canals Obturated with Different Filling Techniques: A Confocal Laser Scanning Microscopy Study

Hirai

Machado²,

Budziak

et al. 2020

Eur J Dent

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Objective This study compared different obturation techniques, analyzing percentage of areas filled with gutta-percha, sealer, and voids (PGFA, PSFA, and PVFA, respectively) in oval-shaped root canals. Materials and Methods A total of 60 extracted human mandibular central incisors were decoronated, instrumented, and irrigated using the same protocol. After drying, the root canal was filled with AH Plus labeled with 0.1% rhodamine B dye using a Lentulo spiral. The filling procedure was performed by dividing the teeth into four groups according to the respective technique: G1, cold lateral condensation; G2, continuous wave of condensation; G3, modified cold lateral condensation using an F3 master cone; and G4, modified continuous wave of condensation using an ISO (International Organization for Standardization) sized 30 gutta-percha cone. Then, slices measuring 1.5 mm in thickness were obtained 3 and 6 mm from the apex and evaluated by confocal laser scanning microscopy to determine PGFA, PSFA, and PVFA. Statistical Analysis The data were analyzed statistically with analysis of variance and Games-Howell’s tests (p = 0.05). Results The groups showed no significant differences in the apical third (3 mm from the apex). In the middle third (6 mm from the apex), G3 and G1 showed higher PGFA and PVFA, respectively. G3 showed lower PSFA than G2 and G4. Both cold techniques (G1 and G3) promoted lower PSFA than both warm techniques (G2 and G4). Conclusions Notwithstanding the limitations of this in vitro study, PGFA, PSFA, and PVFA ranged significantly only in the middle third, as observed by the different filling techniques. Higher PGFA and PVFA values were obtained for G3 and G1, respectively. Both cold techniques promoted lower PSFA than both warm techniques.

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Comparison of two observational methods, scanning electron and confocal laser scanning microscopies, in the adhesive interface analysis of endodontic sealers to root dentine

Abstract: A proper interface formed between sealer and dentine is very important for final quality of root canal filling. Observational methods which allow an accurate analysis of this interface must be selected to assess such features.

Cited by 34 publications

References 30 publications

Correlation between Bond Strength to Dentin and Sealers Penetration by Push-Out Test and CLSM Analysis

Correlation between Bond Strength to Dentin and Sealers Penetration by Push-Out Test and CLSM Analysis

Effect of peracetic acid used as single irrigant on the smear layer, adhesion, and penetrability of AH Plus

Percentage of Gutta-Percha-, Sealer-, and Void-Filled Areas in Oval-Shaped Root Canals Obturated with Different Filling Techniques: A Confocal Laser Scanning Microscopy Study

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