Comparison of Tyrosinase-related Protein-2, S-100, and Melan A Immunoreactivity in Canine Amelanotic Melanomas

Choi, Changsun; Kusewitt, Donna F.

doi:10.1354/vp.40-6-713

Cited by 45 publications

(50 citation statements)

References 13 publications

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“…The present tumor showed rosettes and immunoreactivities to S-100 protein and NSE, indicative of neurogenic origin. Although neuroectodermal tumors including melanomas may be positive for S-100 protein and NSE, Melan-A is specific for melanocytic tumors [1]. The present tumor cells did not react to Melan A. Ultrastructural findings such as microtubules, intermediate filaments or dense core secretary granules have been reported as a criterion for olfactory neuroblastomas [4], although not consistent findings.…”

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confidence: 51%

Olfactory Neuroblastoma in a Horse

et al. 2006

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ABSTRACT. An 11-year-old thoroughbred gelding was euthanatized because of right nasal cavity tumor. The tumor consisted of round to oval cells with a scanty cytoplasm and hyperchromatic nuclei. Homer-Wright rosettes and pseudorosettes, as well as microcysts were seen. Neoplastic cells were immunoreactive to vimentin, S-100 protein, and neuron-specific enolase, glial fibrillary acidic protein and microtube-associated protein in varying degrees, indicating neurogenic nature. Based on these findings, this tumor was diagnosed as an olfactory neuroblastoma. Since this type is an uncommon tumor showing histological variety, the nature is discussed. KEY WORDS: equine esthesioneuroblastoma, immunohistochemistry, sinonasal tumor.

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confidence: 51%

Olfactory Neuroblastoma in a Horse

et al. 2006

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“…Besides these differences, the IHC for actin showed no positive staining, which would be expected of hemangiopericytomas (Gaitero et al 2008). Regarding melanomas, which have cells that share the same embryological origin at the neural crest, as the Schwann cells (Banks 1992) tend to be positive to markers such as Melan-A and tyrosinase protein-2 (Choi & Kusewitt 2003).…”

Section: Discussionmentioning

confidence: 93%

Retrospective canine skin peripheral nerve sheath tumors data with emphasis on histologic, immunohistochemical and prognostic factors

et al. 2015

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In this retrospective study was determined the frequency of canine skin peripheral nerve sheath tumors (PNST) in cases diagnosed by the Setor de Patologia Veterinária of the Universidade Federal do Rio Grande do Sul (SPV-UFRGS), Brazil, between the years 2000 and 2012. The canine profiles, as well as histological, immunohistochemical and prognostic aspects of the tumors were based on 70 samples, comprising 40 females, 29 males and one unspecified sample. Between 2000 and 2012, 2,984 skin tumors of dogs were diagnosed in the SPV-UFRGS, totaling 2.34% of skin neoplasms in dogs. Animals that comprised the largest amount of samples (43%) were those with no breed (SRD), followed by German Shepherds (10%). Females were more affected than males (40/70 - 57% and 29/70 - 41% respectively). Skin PNST of this research showed predominant localization on the limbs (40% in the forelimbs and 29% in the hindlimbs); affecting adult dogs, mostly aged between 8 and 11 years (54%). The samples were routinely processed for hematoxylin and eosin, and were also evaluated by toluidine blue and Masson's trichrome staining, and immunohistochemistry (IHC) anti-vimentin, -S-100, -GFAP, -actin, von Willebrand factor and neurofilament. Anisocytosis and anisokaryosis, mitotic index, intratumoral necrosis, invasion of adjacent tissues, tumor location, local recurrence and metastasis were related to the diagnosis of benign (49/70) or malignant tumor (21/70). The Antoni A histological pattern was observed more frequently in benign tumors. The immunohistochemistry helped to diagnose PNST, and anti-vimentin and anti-protein S-100 showed the highest rates of immunostaining. Throughout statistical analysis of animals with tumor recurrence, it was found that the chance of an animal with a malignant peripheral nerve sheath tumor to develop recurrence is 4.61 times higher than in an animal that had a benign tumor.

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“…14,17,19 More recently, a number of novel mAbs that recognize formalin-resistant melanocytic differentiation antigens have become available in human and veterinarypathology, including Melan A/MART 1 (clone A103/M2-7C10), anti-tyrosinase (T311), and microphthalmia transcription factor (D5). 4,6,7,9 Melan A/MART 1 is one of the most extensively used mAbs in both human and veterinary pathology diagnostics. It is a melanocyte-differentiating protein that is recognized by tumor-infiltrating human cytotoxic T lymphocytes.…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical Investigation of PNL2 Reactivity of Canine Melanocytic Neoplasms and Comparison with Melan A

et al. 2010

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Abstract. PNL2 is a recently generated monoclonal antibody (mAb) that recognizes normal and neoplastic melanocytes. Although the antigen recognized by PNL2 remains unknown, recent studies of human and mouse melanomas have confirmed its usefulness as a diagnostic marker. In the current study, the immunoreactivity of PNL2 in canine melanomas was tested and compared with Melan A (A103). Validation of PNL2 was performed by Western blot analysis. PNL2 and Melan A immunoreactivity were tested on frozen samples of canine melanomas and on 69 formalin-fixed, paraffin-embedded melanocytic neoplasms. Normal canine tissues and nonmelanocytic neoplasms were included as negative controls. Western blot confirmed the presence of a protein recognized by the PNL2 antibody in canine melanomas. Immunohistochemically, PNL2 stained the melanocytic neoplastic cells with an intracytoplasmic, granular pattern. Among the melanocytic neoplasms tested, 62% stained positively with PNL2 and 59% with Melan A; 50.7% stained positively with both mAbs. The overall percentage of neoplasms that stained positively with at least 1 of these 2 antibodies was 68%. The extent of staining (i.e., the percentage of cells stained per specimen) was greater with PNL2 than with Melan A. With both mAbs, staining was most intense and diffuse in the epithelioid cell phenotype. Neither nonspecific staining nor staining in cells other than melanocytes was detected with either mAb. In contrast to human granulocytes, canine granulocytes were negative by both Western blot and immunohistochemical analyses. PNL2 mAb proved to be highly specific for the identification of formalinfixed canine melanocytic neoplasms and should be a valuable diagnostic reagent.

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Comparison of Tyrosinase-related Protein-2, S-100, and Melan A Immunoreactivity in Canine Amelanotic Melanomas

Cited by 45 publications

References 13 publications

Olfactory Neuroblastoma in a Horse

Olfactory Neuroblastoma in a Horse

Retrospective canine skin peripheral nerve sheath tumors data with emphasis on histologic, immunohistochemical and prognostic factors

Immunohistochemical Investigation of PNL2 Reactivity of Canine Melanocytic Neoplasms and Comparison with Melan A

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