Comparison of Urinary Total Proteins by Four Different Methods

Yalamati, Padma; Karra, Madhu Latha; Bhongir, Aparna Varma

doi:10.1007/s12291-016-0551-3

Cited by 15 publications

(14 citation statements)

References 11 publications

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“…As previously described in the literature, quantitative methods for urinary proteins are not mutually comparable and none of the available methods is considered as a “gold standard” method ( 9 ). In our study, the agreement of dipsticks was better with turbidimetric method for total urinary protein.…”

Section: Discussionmentioning

confidence: 99%

“…Since there is no recommendation for a reference method for urinary total protein measurement, and given the large differences between these two methods, dipstick results for proteins were compared with quantitative measurements by two methods (pyrogallol red molybdate and benzethonium chloride) separately ( 9 ). Furthermore, dipstick results for glucose were compared to mean value of all three chemistry analysers.…”

Section: Methodsmentioning

confidence: 99%

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Analytical verification of 12 most commonly used urine dipsticks in Croatia: comparability, repeatability and accuracy

Vuljanić¹,

Dojder²,

Špoljarić³

et al. 2018

Biochem. med. (Online)

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IntroductionVariability among manufacturers of urine dipsticks, respective to their accuracy and measurement range, may lead to diagnostic errors and thus create a serious risk for the patient. Our aims were to determine the level of agreement between 12 most commonly used urine dipsticks in Croatia, examine their accuracy for glucose and total protein and to test their repeatability.Materials and methodsA total of 75 urine samples were used to examine comparability and accuracy of 12 dipstick brands (Combur 10 TestM, ChoiceLine 10, Combur 10 TestUX, ComboStik 10M, ComboStik 11M, CombiScreen 11SYS, CombiScreen 10SL, Combina 13, Combina 11S, Combina 10M, UriGnost 11, Multistix 10SG). Agreement between each dipstick and the reference (Combur 10 TestM) was expressed as kappa coefficient (acceptable κ ≥ 0.80). Accuracy for glucose and total protein was tested by comparison with quantitative measurements on analysers: AU400 (Beckman Coulter, USA), Cobas 6000 c501 (Roche Diagnostics, Germany) and Architect plus c4000 (Abbott, USA). Repeatability was assessed on 20 replicates (acceptable > 90%).ResultsBest agreement was achieved for glucose, total protein and nitrite (11/11, k > 0.80) and the lowest for bilirubin (5/5, k < 0.60). Sensitivities for total protein were 41-75% (AU400) and 56-92% (Cobas and Architect); while specificities were 41-75% (AU400, Cobas, Architect). Dipsticks’ sensitivity and specificity for glucose were 68-98%. Most of the dipsticks showed unacceptable repeatability (6/12, < 90%) for one parameter, most prominently for pH (3/12, < 90%).ConclusionsMost commonly used dipsticks in Croatia showed low level of agreement between each other. Moreover, their repeatability varies among manufacturers and their accuracy for glucose and proteins is poor.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Analytical verification of 12 most commonly used urine dipsticks in Croatia: comparability, repeatability and accuracy

Vuljanić¹,

Dojder²,

Špoljarić³

et al. 2018

Biochem. med. (Online)

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show abstract

“…Urine total protein concentration indicates renal function and many human diseases . It is often measured by the pyrogallol red‐molybdate (PRM) assay in clinical laboratories . Although this simple, rapid, and cost‐effective method provides a wide linear detection range, it is often subject to the interference in the urine samples , giving rise to inaccurate and sometimes contradictory results.…”

Section: Introductionmentioning

confidence: 99%

Urine protein quantification in stacking gel by SDS‐PAGE

Jia

Pan

et al. 2018

Electrophoresis

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Urine total protein concentration is usually measured by the pyrogallol red‐molybdate (PRM) assay in clinical laboratories, but it is often subject to sample interference. Here, we introduce a stacking gel‐based method for accurate protein quantitation. In this method, the urine protein samples are run into the stacking gel by SDS‐PAGE where it is concentrated into a single band, and then quickly stained by 0.001% Coomassie at high temperature. High correlations were found between the BSA and urine protein standards (R2 = 0.997 and 0.990, respectively). Addition of 80 ng urine protein standard into each of the ten clinical urine specimens with questionable PRM results yielded the expected increase in the results by this method. Comparison of the PRM method and with the gel quantitation approach on about 60 clinical urine samples demonstrated a general consistency between the results (R2 = 0.825), but in PRM samples with lower protein concentration showed more variations. Overall, the stacking gel method might be a good alternative for clinical urine samples with suspicious protein concentration results.

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“…Данный метод позволяет выявить протеину-рию у всех беременных с преэклампсией, тогда как тест-полоски недооценивают степень про-теинурии в 20-30 % случаев, а метод с ССК -в 50-60 % случаев [4][5][6][7][8]. В 2015 г. в РФ пред-ложен референтный интервал протеинурии, полученный для колориметрического метода определения белка в моче с использованием ПГК, для беременных женщин вне зависимо-сти от триместра беременности, равный 0,02-0,15 г/л в суточной моче [9].…”

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Compared diagnostic value of proteinuria definition applying different methods at pregnants with preeclampsia

et al. 2017

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Colorimetric method of protein determination in urine with use of pyrogallol red (PGR) has certain advantages over a routine method of proteinuria determination with use of sulfosalicylic acid (SSA) which is still applied in most clinics in the Russian Federation. For the purpose to determinate the diagnostic significance of proteinuria estimation by different methods at pregnants with preeclampsia, conducted a comparative research of proteinuria levels at 68 pregnants with preeclampsia and at 15 healthy pregnant by two methods: with sulfosalicylic acid and with pyrogallol red. At the same patients the diagnostic significance of protein/creatinine ratio was evaluated when using the method with PGR. It was revealed that proteinuria determination with PGR- method had authentically higher sensitivity, and proteinuria indices when using this method corresponds to the criteria for evaluation of preeclampsia severity specified in the Ministry of Health Russian Federation Order No. 572n 2012 and the Clinical Recomandations “Hypertensia during pregnancy. Preeclampsia. Eclampsia” (2012). In case of proteinuria determination by method with SSK it is necessary to consider lower sensitivity of the method and adhere to earlier classification of preeclampsia (gestosis) where proteinuria more 1 g/l was regarded as the symptom of severe preeclampsia. Confirmation of the diagnostic significance of protein/creatinine ratio needs further researches.

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Comparison of Urinary Total Proteins by Four Different Methods

Cited by 15 publications

References 11 publications

Analytical verification of 12 most commonly used urine dipsticks in Croatia: comparability, repeatability and accuracy

Analytical verification of 12 most commonly used urine dipsticks in Croatia: comparability, repeatability and accuracy

Urine protein quantification in stacking gel by SDS‐PAGE

Compared diagnostic value of proteinuria definition applying different methods at pregnants with preeclampsia

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