Comparison of uteroplacental glycosylation in the camel (Camelus dromedarius) and alpaca (Lama pacos)

Jones, Carolyn J.P.; Abd-Elnaeim, M.; Bevilacqua, Estela; Oliveira, LC; Leiser, Rudolf

doi:10.1530/rep.0.1230115

Cited by 32 publications

(31 citation statements)

References 29 publications

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“…Generally speaking, the morphological and histochemical characteristics of the areolae in our study were very similar to those described in peccaries (Santos 2006), sows (Dantzer 1984) and alpacas (Olivera 2002, Olivera et al 2003a. The classification of regular and irregular areolae as observed in sows was not found in the llama (Abd-Elnaeim et al 2003).…”

Section: Discus Discus Discus Discus Discussion Sion Sion Sion Sionsupporting

confidence: 85%

“…As regards the epidermal membrane of llamas, the morphological characteristics match the descriptions already made for llamas (Fowler & Olander 1990, Olivera 2002 and also for other members of the Camelidae family (Musa 1977, Merkt et al 1988. Based on the structural results it can be stated that this membrane has a mechanical role of protecting the internal surface of the fetal membranes as well as facilitating llamas' parturition.…”

Section: Discus Discus Discus Discus Discussion Sion Sion Sion Sionsupporting

confidence: 74%

“…This type of placenta is also similar in mares and sows, although the fetal membranes of the camelids have particular characteristics (Stevens et al 1980, Zhang et al 1991, Fowler & Bravo 1998. As stated by some authors (Abd-Elnaeim et al 1999, Jones et al 2002, Olivera 2002, Olivera et al 2003a, Wooding et al 2003 very large cells which contain large nuclei and numerous nucleoli in the trophoblastic layer of alpacas, Bactrian camels and dromedaries have been found.…”

Section: Introduction Introduction Introduction Introduction Introducmentioning

confidence: 71%

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The materno-fetal interface in llama (Lama guanicoe glama)

et al. 2007

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Samples from 9 llamas (28 through 36 weeks of gestation) were collected and fixed in 4% buffered paraformaldehyde (light microscopy) and in 2.5% buffered glutaraldehyde (transmission and scanning electron microscopy). The material was processed in paraplast and slides (5mm) were stained with HE, PAS, Masson-Trichrome, acid phosphatase and Perl's. The uteroferrin was immunolocalized. The results show that llama placenta is chorioallantoic, diffuse, folded and epitheliochorial, and the fetus is covered with an epidermal membrane. The trophoblast cells have variable morphology: cubic, rounded and triangular cells, with cytoplasm containing PAS-positive granules. Binucleated cells with large cytoplasm and rounded nuclei, as well as giant trophoblastic cells with multiple nuclei were also observed. Numerous blood vessels were observed beneath the cells of the uterine epithelium and around the chorionic subdivided branches. Glandular activity was shown by PAS, Perl's, and acid phosphatase positive reactions in the cytoplasm and glandular lumen, and by immunolocalization of the uteroferrin in the glandular epithelium. The uterine glands open in spaces formed by the areoles, which are filled by PAS-positive material. The llama fetus was covered by the epidermal membrane, composed of stratified epithelium, with up to seven layers of mono-, bi- or trinucleated cells. The high level of maternal and fetal vascularization surfaces indicates an intense exchange of substances across both surfaces. The metabolic activity shown in the uterine glands suggests an adaptation of the gestation to the high altitudes of the natural habitat of this species.

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Section: Discus Discus Discus Discus Discussion Sion Sion Sion Sionsupporting

confidence: 85%

Section: Discus Discus Discus Discus Discussion Sion Sion Sion Sionsupporting

confidence: 74%

Section: Introduction Introduction Introduction Introduction Introducmentioning

confidence: 71%

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The materno-fetal interface in llama (Lama guanicoe glama)

et al. 2007

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“…. ' (Jones et al, 2002). Our limited observations indicate a similar frequency per unit length of trophoblast epithelium throughout pregnancy in dromedary camels, but an accurate morphometric study is required.…”

Section: Discussionmentioning

confidence: 83%

“…Giant multinucleate Email: fbpw2@cam.ac.uk cells develop in the trophoblast between day 30 and day 35 of pregnancy, and are found scattered through the trophoblast at a similar frequency throughout the remainder of gestation (Skidmore et al, 1996b). The presence of giant cells has been reported in the placentae of old (Van Lennep, 1963;Gorokhovski et al, 1975;Skidmore et al, 1996b;Jones et al, 2002) and new (Jones et al, 2002) world camelids, but the detailed structure and metabolic function of these cells has not been investigated. This paper reports an immunohistochemical and ultrastructural study of uninucleate and giant trophoblast cells throughout pregnancy in camelid placentae, which indicates that they are analogous to rodent giant cells in their capacity for steroid production.…”

Section: Introductionmentioning

confidence: 99%

Developmental changes in localization of steroid synthesis enzymes in camelid placenta

Wooding

Öztürk²,

Skidmore³

et al. 2003

Reproduction

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The uninucleate trophoblast of epitheliochorial camelid placenta produces multinucleate giant cells starting between day 30 and day 35 of pregnancy. The giant cells are found scattered along the trophoblast at similar frequency throughout gestation. Light microscope immunocytochemistry indicates that the four steroid synthesis enzymes (cholesterol side chain cleavage, 3␤-hydroxysteroid dehydrogenase, 17␣-hydroxylase and aromatase) are present in all uninucleate trophoblast cells between day 14 and day 30 of pregnancy, but are found only in the giant cells once they are established, and that this localization persists until term. The giant cells show massive amounts of smooth endoplasmic reticulum and numerous small mitochondria, again as has been shown in other steroidproducing cells. As progesterone from the corpus luteum is necessary throughout gestation in camels, the capacity for oestrogen production by the trophoblast presumably has an important role, but one limited mostly to the immediate vicinity of the fetomaternal interface.

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Placental glycotype of the caviomorph rodent Lagostomus maximus and its evolution within Eutheria

Acuña

Carril

Portiansky

et al. 2023

Journal of Morphology

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The main evolutionary milestone in the oviparity-viviparity transition is placentation.The placenta is an organ with great morphological diversity among eutherians. The expression of different glycosidic residues (Gr) in the near-term placenta constitutes its glycotype. In this study, the expression of different Gr was determined by lectin histochemistry in early, midterm, and near-term placentas of the plains viscacha (Lagostomus maximus), a caviomorph rodent with the highest poliovulatory rate and embryonic resorption rate among eutherians. Besides, a matrix with the expression of each Gr in the exchange trophoblast of viscacha and other eutherians was constructed to map and infer phylogenetic and evolutionary relationships. Between early, midterm, and near-term placentas, variations in the pattern expression of Gr were observed. The glycotype of the near-term placenta is composed of a high diversity of Gr. Reconstruction of the ancestral state for each Gr present in the nearterm placenta showed a diverse scenario: some sugars were common to the species of Placentalia included in this study. In the analyzed species with synepitheliochorial and epitheliochorial placentas, no differential glycosylation patterns between them were observed. In species with invasive placentas, such as the endotheliochorial placentas of Carnivora, some common Gr were detected among them, while others were species-specific. In species with hemochorial placenta, the same Gr are shared.Particularly, in the viscacha greater differences with species of the Hominidae and even Muridae families were observed. Nevertheless, greater similarities with other caviomorph rodents were detected. Placental glycotype of each species constitutes an excellent tool to achieve phylogenetic and evolutionary inferences among eutherians.

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Comparison of uteroplacental glycosylation in the camel (Camelus dromedarius) and alpaca (Lama pacos)

Cited by 32 publications

References 29 publications

The materno-fetal interface in llama (Lama guanicoe glama)

The materno-fetal interface in llama (Lama guanicoe glama)

Developmental changes in localization of steroid synthesis enzymes in camelid placenta

Placental glycotype of the caviomorph rodent Lagostomus maximus and its evolution within Eutheria

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