Comparison of various extraction techniques for isolation and determination of isoflavonoids in plants

Abstract

In the present paper, the following extraction techniques have been used for extracting isoflavonoids from the species Matricaria recutita, Rosmarinus officinalis, Foeniculum vulgare, and Agrimonia eupatoria L.: supercritical fluid extraction (SFE), pressurized fluid extraction, matrix solid phase dispersion, ultrasonic extraction in an ultrasonic bath (USE) and by means of an ultrasonic homogeniser (HOM), extraction by means of Soxhlet apparatus (SOX), and solid phase extraction. Experimental optimization of all techniques has been carried out using a soybean flour. Subsequent analyses of the extracts were carried out by liquid chromatography with UV detection. The maximum yields of daidzein and genistein were obtained by extraction with the SOX, USE, and HOM techniques. The maximum yields of apigenin and biochanin A from herb samples were obtained by SFE.

Preparation of Botanical Samples for Biomedical Research

“…But even without the markers, one can still use this method to quickly prepare crude extracts for bioactivity screening. Anand et al [17] compared three methods including PLE for extracting the bioactive molecules in Hypericum perforatum L. PLE was found to be more effective in extracting caffeine, phytol, palmitic, and stearic acid in mate tea leaves (Ilex paraguariensis) and in shortening the total extraction time and reducing solvent consumption [18], the same conclusions drawn by many other investigators [19][20][21][22]. PLE was also effective in extracting terpenes, fatty acids and Vitamin E [23] as well as giving a high total extract yield [24] but less effective in extracting sterols [25] and caffeine from green tea leaves [26].…”

Extraction of antioxidants from plants using ultrasonic methods and their antioxidant capacity

“…First of all the suitable solvent was selected. Because of our previous experiences [20] only the mixtures ACN -water at various ratios were tested. Based on the obtained results (not presented) the 30% v/v ACN was selected as the extraction solvent and was used for further experiments.…”

Phytochemical analysis of isoflavonoids using liquid chromatography coupled with tandem mass spectrometry

“…In addition to the traditional extraction methods i.e. maceration, percolation, soxhilation which have been used for decades, newer techniques like accelerated solvent extraction, extraction using ultra sonication, microwave assisted extraction (Rostagno et al 2007), supercritical fluid extraction (Cheng et al 2011;Klejdus et al 2005aKlejdus et al , 2010Rostagno et al 2002), ionic liquid based ultrasonic assisted extraction (ILUAE), solid phase extraction (SPE) and pressurized liquid extraction (PLE) (Bajer et al 2007;Rostagno et al 2004) have been increasingly reported for the isolation of isoflavonoids from plant products. These techniques were developed for application in natural product extraction owing to their advantages with regard to the extraction time, reproducibility and better yields due to elevated pressure and temperature which lead to better penetration of extraction solvent to the matrix pores.…”

“…heartwood, seeds, leaves, rhizomes, roots etc. The need for sample preparation depends strongly on the sample type and the analysis techniques to be employed (Bajer et al 2007). Extraction technique, extraction solvent, pH of the solvent, extraction time, temperature, sample to solvent ratio, sample characteristics are the defining factors that affect extraction efficiency, isoflavonoids profile and recovery (Rostagno et al 2010).…”