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In the present paper, the following extraction techniques have been used for extracting isoflavonoids from the species Matricaria recutita, Rosmarinus officinalis, Foeniculum vulgare, and Agrimonia eupatoria L.: supercritical fluid extraction (SFE), pressurized fluid extraction, matrix solid phase dispersion, ultrasonic extraction in an ultrasonic bath (USE) and by means of an ultrasonic homogeniser (HOM), extraction by means of Soxhlet apparatus (SOX), and solid phase extraction. Experimental optimization of all techniques has been carried out using a soybean flour. Subsequent analyses of the extracts were carried out by liquid chromatography with UV detection. The maximum yields of daidzein and genistein were obtained by extraction with the SOX, USE, and HOM techniques. The maximum yields of apigenin and biochanin A from herb samples were obtained by SFE.

The mixed dissociation constants of five drugs-clotrimazole, terbinafine HCl, acetylsalicylic acid, salicylic acid, and galanthamineat various ionic strengths I and at temperatures of 25 • C and 37 • C were determined with the use of multiwavelength and multivariate treatments of spectral data SPECFIT/32 nonlinear regression analysis and INDICES factor analysis. The factor analysis in the INDICES program predicts the number of components, when the data quality is high and the instrumental error is known. The thermodynamic dissociation constant pK T a was estimated by nonlinear regression of {pK a, I} data at 25 • C and 37 • C: for clotrimazole pK T a,1 = 4.38(1) and 4.16(3); for terbinafine HCl pK T a,1 = 4.19(3) and 4.12(5); for acetylsalicylic acid pK T a,1 = 3.49(25) and 3.41(15); for salicylic acid pK T a,1 = 3.01(1) and 3.00(1) and for galanthamine pK T a,1 = 8.21(1) and 7.99(2) where in brackets the standard deviation is in the last significant digits. Goodness-of-fit tests for various regression diagnostics enabled the reliability of the parameter estimates found to be proven. Pharma Algorithms predicts pK a being based on the structural formulae of drug compounds.

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