1987
DOI: 10.1016/0141-0229(87)90008-1
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Comparison of various permeabilization treatments on Kluyveromyces by determining in situ β-galactosidase activity

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Cited by 39 publications
(16 citation statements)
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“…Different procedures are reported in literature to achieve the contact between β-galactosidase with substrate (Decleire et al, 1987, Flores et al, 1994, Kippert, 1995, but in our experiments better results were obtained by treated cells with ethanol (Lee et al, 2004). The set up of cell treatment was carried out by evaluating the experimental conditions, in particular time effect (from 5 min to 4 h), temperature (from 5 to 20 °C) and ethanol concentration (from 5 to 60% v/v).…”
Section: Preliminary Screeningmentioning
confidence: 99%
“…Different procedures are reported in literature to achieve the contact between β-galactosidase with substrate (Decleire et al, 1987, Flores et al, 1994, Kippert, 1995, but in our experiments better results were obtained by treated cells with ethanol (Lee et al, 2004). The set up of cell treatment was carried out by evaluating the experimental conditions, in particular time effect (from 5 min to 4 h), temperature (from 5 to 20 °C) and ethanol concentration (from 5 to 60% v/v).…”
Section: Preliminary Screeningmentioning
confidence: 99%
“…The freezing and thawing procedure was repeated for different numbers of cycles (one, five, 10, 15 and 20). The permeabilized cells were washed with buffer and taken for assay (Decleirie et al 1987).…”
Section: Physical Methods Of Permeabilizationmentioning
confidence: 99%
“…The polysaccharide, chitosan (Jaspers et al 1975), and an antibiotic, nystatin (Bechet and Wiame 1965), did not increase enzyme activity (Table 2). Decleirie et al (1987) and Borralho et al (1983) found that the use of freeze-thaw cycles resulted in maximum activity of b-galacotsidase and 5-aminolevulinate dehydratase, respectively, in yeasts. The enzyme activity was found to be low in yeast suspensions compared with pellets subjected to freeze-thaw cycles.…”
Section: -----------------------------------------------------mentioning
confidence: 99%
“…Different organic solvents for permeabilization of K. bulgaricus IRC 101 cells for bgalactosidase activity have been reported [15]. The maximum efficacy has been reported with 30% isopropanol, 40% tert-butanol and 70% ethanol.…”
Section: Permeabilization Of Yeast Cellsmentioning
confidence: 93%
“…It has been reported that the permeabilizing agents act on the cell membranes by decreasing the phospholipid content [18]. When organic solvents were used as permeabilizing agents, up to 30%, decrease of phospholipid content in yeasts have been reported [15]. It is believed that the digitonin forms a complex with cholesterol present in the cell membrane and renders the membrane leakage.…”
Section: Permeabilization Of Yeast Cellsmentioning
confidence: 98%