Influence of substrate on β-galactosidase production byKluyveromyces strains

Rollini, Manuela; Trinetta, Valentina; Musatti, Alida; Manzoni, M.

doi:10.1007/bf03175578

Cited by 4 publications

(2 citation statements)

References 18 publications

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“…Compositions of various media gave results contradicting with the comparative study carried out by Rollini et al [23]. Although Rollini et al [23] reported that the application of cheese whey as a carbon source will result in about 8-to10-fold increase in cell growth of K. lactis than lactose, our experimental results (Fig. 1) showed the opposite.…”

Section: Cultivations In Different Mediacontrasting

confidence: 91%

Bioprocess Development for High Cell Mass Production of the Probiotic Yeast-Kluyveromyces lactis

C.H¹

2013

IOSR-JPBS

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: In the present study, the key industrial strain of Kluyveromyces lactis NRRL Y-110 was used todevelop an industrial process for probiotic cell mass production. Therefore, the biomass production process of K. lactis was developed via the optimization of different medium and process parameters in shake flask and bioreactor levels. In the first part of the work, the effect of medium composition on the production of biomass was investigated in shake flask culture.

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Section: Cultivations In Different Mediacontrasting

confidence: 91%

Bioprocess Development for High Cell Mass Production of the Probiotic Yeast-Kluyveromyces lactis

C.H¹

2013

IOSR-JPBS

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“…The excellent tolerance of the K. marxianus PYCC 3286 strain to ethanol has been recently noted by other authors who showed that this strain can tolerate up to 79 g/L of ethanol [43]. Moreover, a high beta-galactosidase specific activity was also reported for this strain (3207 IU/g DW) [44]. It is conceivable that the combination of high ethanol tolerance and high beta-galactosidase activity are decisive features for the superior fermentative behavior displayed by the K. marxianus PYCC 3286 strain in the NF and RO retentates, both under mild and severe oxygen-limiting conditions.…”

Section: Resultssupporting

confidence: 55%

Integrated Process for Bioenergy Production and Water Recycling in the Dairy Industry: Selection of Kluyveromyces Strains for Direct Conversion of Concentrated Lactose-Rich Streams into Bioethanol

et al. 2019

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Dairy industries have a high environmental impact, with very high energy and water consumption and polluting effluents. To increase the sustainability of these industries it is urgent to implement technologies for wastewater treatment allowing water recycling and energy savings. In this study, dairy wastewater was processed by ultrafiltration and nanofiltration or ultrafiltration and reverse osmosis (UF/RO) and retentates from the second membrane separation processes were assessed for bioenergy production. Lactose-fermenting yeasts were tested in direct conversion of the retentates (lactose-rich streams) into bioethanol. Two Kluyveromyces strains efficiently fermented all the lactose, with ethanol yields higher than 90% (>0.47 g/g yield). Under severe oxygen-limiting conditions, the K. marxianus PYCC 3286 strain reached 70 g/L of ethanol, which is compatible with energy-efficient distillation processes. In turn, the RO permeate is suitable for recycling into the cleaning process. The proposed integrated process, using UF/RO membrane technology, could allow water recycling (RO permeate) and bioenergy production (from RO retentate) for a more sustainable dairy industry.

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ENHANCED β-GALACTOSIDASE PRODUCTION OFAspergillus oryzaeMUTATED BY UV AND LiCl

Zhang

Liu

Min

et al. 2013

Preparative Biochemistry and Biotechnology

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In order to breed a high-yield β-galactosidase-producing strain, Aspergillus oryzae was used as the parent strain and mutagenized with ultraviolet (UV) and UV plus lithium chloride (LiCl), respectively. After being mutagenized by UV, the β-galactosidase activity of mutant UV-15-20 reached 114.08 U/mL, which revealed a 49.22% increase compared with the original strain. A mutant UV-LiCl-38 with high β-galactosidase activity (121.42 U/mL) was obtained after compound mutagenesis of UV and LiCl; the β-galactosidase activity of this mutant was 58.82% higher than that of the parent strain. Subculture testing indicated that UV-15-20 and UV-LiCl-38 had good hereditary stability and may be ideal strains for the production of β-galactosidase. Additionally, it was demonstrated that compound mutagenesis with UV and LiCl is an effective mutation method for breeding industrially interesting strains.

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Influence of substrate on β-galactosidase production byKluyveromyces strains

Cited by 4 publications

References 18 publications

Bioprocess Development for High Cell Mass Production of the Probiotic Yeast-Kluyveromyces lactis

Bioprocess Development for High Cell Mass Production of the Probiotic Yeast-Kluyveromyces lactis

Integrated Process for Bioenergy Production and Water Recycling in the Dairy Industry: Selection of Kluyveromyces Strains for Direct Conversion of Concentrated Lactose-Rich Streams into Bioethanol

ENHANCED β-GALACTOSIDASE PRODUCTION OFAspergillus oryzaeMUTATED BY UV AND LiCl

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