Comparison of Venom Extraction Methods of Bombus (Bombus) terrestris (Hymenoptera: Apidae: Bombus) by Using Bottom-up Proteomic Strategies

Barkan, Nezahat Pinar; Demiralp, Fatma Duygu Özel; Aytekin, A. Murat

doi:10.2174/157016461202150903111853

Cited by 2 publications

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“…and enhance phospholipase A2 in liposome hydrolysis, in a similar fashion to melittin found in A. mellifera venom and crabrolin and mastoparan found in vespid Vespa crabro venom (Argiolas & Pisano, 1985). Phospholipase A2 (Hoffman, El-Choufani, Smith, & De Groot, 2001;Hoffman & Jacobson, 1996;Van Vaerenbergh, Debyser, Smagghe, Devreese, & de Graaf, 2015), serine proteases (Choo, Lee, Yoon, Kim, et al, 2010;Choo et al, 2011;Kim et al, 2013;Qiu et al, 2011;Qiu et al, 2012b;Van Vaerenbergh et al, 2015) , serine protease inhibitors (Qiu et al, 2013;Wan et al, 2014), acid phosphatases (Hoffman et al, 2001;Hoffman & Jacobson, 1996;Van Vaerenbergh et al, 2015), arginine kinase (N. P. Barkan, Demiralp, & Aytekin, 2015), hyaluronidase (Hoffman & Jacobson, 1996), putrescine (Tom Piek, 2013), citrate (Fenton et al, 1995), defensin (Rees, Moniatte, & Bulet, 1997), and acethylcoline (T Piek, Veldsema-Currie, Spanjer, & Mantel, 1983) have also been isolated from Bombus sp. venom.…”

Section: Introductionmentioning

confidence: 69%

Proteomic Characterization of the Venom Of FiveBombus (Thoracobombus)Species

Barkan

Bayazit

Demiralp

2017

Preprint

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Venomous animals use venom; a complex biofluid composed of unique mixtures of proteins and peptides, to act on vital systems of the prey or predator. In bees, venom is solely used for defense against predators. However, the venom composition of bumble bees (Bombus sp.) is largely unknown. Thoracobombus subgenus of Bombus sp. is a diverse subgenus represented by 14 members across Turkey. In this study, we sought out to proteomically characterize the venom of five Thoracobombus species by using bottom-up proteomic techniques. We have obtained two-dimensional polyacrylamide gel (2D-PAGE) images of each venom sample. We have subsequently identified the protein spots by using matrix assisted laser desorption zonatus. Our analyses provide the primary proteomic characterization of five bumble bee species' venom composition.

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Section: Introductionmentioning

confidence: 69%

Proteomic Characterization of the Venom Of FiveBombus (Thoracobombus)Species

Barkan

Bayazit

Demiralp

2017

Preprint

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“…and enhance phospholipase A 2 in liposome hydrolysis, in a similar fashion to melittin found in A. mellifera venom and crabrolin and mastoparan found in vespid Vespa crabro venom [ 17 ]. Phospholipase A 2 [ 18 , 19 , 20 ], serine proteases [ 6 , 8 , 10 , 11 , 13 , 20 ], serine protease inhibitors [ 14 , 15 ], acid phosphatases [ 18 , 19 , 20 ], arginine kinase [ 21 ], hyaluronidase [ 19 ], putrescine [ 22 ], citrate [ 23 ], defensin [ 24 ], and acethylcoline [ 25 ] have also been isolated from Bombus sp . venom.…”

Section: Introductionmentioning

confidence: 99%

Proteomic Characterization of the Venom of Five Bombus (Thoracobombus) Species

Barkan

Bayazit

Demiralp

2017

Toxins

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Venomous animals use venom, a complex biofluid composed of unique mixtures of proteins and peptides, to act on vital systems of the prey or predator. In bees, venom is solely used for defense against predators. However, the venom composition of bumble bees (Bombus sp.) is largely unknown. The Thoracobombus subgenus of Bombus sp. is a diverse subgenus represented by 14 members across Turkey. In this study, we sought out to proteomically characterize the venom of five Thoracobombus species by using bottom-up proteomic techniques. We have obtained two-dimensional polyacrylamide gel (2D-PAGE) images of each species’ venom sample. We have subsequently identified the protein spots by using matrix assisted laser desorption ionization/time-of-flight mass spectrometry (MALDI-TOF MS). We have identified 47 proteins for Bombus humilis, 32 for B. pascuorum, 60 for B. ruderarius, 39 for B. sylvarum, and 35 for B. zonatus. Moreover, we illustrated that intensities of 2DE protein spots corresponding to putative venom toxins vary in a species-specific manner. Our analyses provide the primary proteomic characterization of five bumble bee species’ venom composition.

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Comparison of Venom Extraction Methods of Bombus (Bombus) terrestris (Hymenoptera: Apidae: Bombus) by Using Bottom-up Proteomic Strategies

Cited by 2 publications

References 0 publications

Proteomic Characterization of the Venom Of FiveBombus (Thoracobombus)Species

Proteomic Characterization of the Venom Of FiveBombus (Thoracobombus)Species

Proteomic Characterization of the Venom of Five Bombus (Thoracobombus) Species

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