Comparisons of prebiotic activity of polysaccharides from shoot residues of bamboo ( Chimonobambusa quadrangularis ) via different ethanol concentrations

Abstract

Three polysaccharide fractions from bamboo shoot (Chimonobambusa quadrangularis), CPS70, CPS75, and CPS80, were prepared using a final ethanol concentration of 70%, 75%, and 80% in the precipitation process. In vitro digestibility and the prebiotic activity of CPS70, CPS75, and CPS80 were evaluated and compared. The results indicated that all three of the CPS fractions exhibit a high degree of nondigestibility to human gastric juice (>98.5%) or α‐amylase hydrolysis (>94.5%). Compared with the blank control, the three CPS fractions could not only significantly (p < .05) stimulate the proliferation of B. adolescentis, B. infantis, B. bifidum, and L. acidophilus, but also significantly (p < .05) enhance the production of lactic, acetic, propionic, and butyric acids when these polysaccharides were added as alternative carbon sources to glucose during the in vitro fermentation of four probiotics. Furthermore, when comparing the three CPS fractions, CPS75 displayed the strongest prebiotic potential, as this polysaccharide had the strongest effect on the proliferation of probiotic bacteria as well as the greatest effect on SCFAs production. These results demonstrated that the concentration of ethanol used during the precipitation process has a significant impact on the prebiotic activity of CPS. Practical applications Ethanol precipitation is the first step when extracting polysaccharides from aqueous extracts as it is simple, rapid, and easy to carry out. This study focuses on how different concentrations of ethanol used in the precipitation process affect the prebiotic potential of bamboo shoot (Chimonobambusa quadrangularis) polysaccharides (CPS). The result indicated that the concentration of ethanol used during the precipitation process has a significant impact on the prebiotic activity of CPS. To our knowledge, it is the first to evaluate the effects of the concentration of ethanol during the process of precipitation on prebiotic potential of polysaccharides, which can subsequently be applied to the optimization of ethanol concentration when precipitating natural polysaccharides for the purpose of in vitro fermentation.

Isolation of a New Polysaccharide from Dandelion Leaves and Evaluation of Its Antioxidant, Antibacterial, and Anticancer Activities

“…DLP-3 exhibited a smooth surface and a purely flake-like structure. The apparent structure of polysaccharides can determine their physical and chemical properties [ 26 ]. The smooth flake-like structure of DLP-3 may be related to its antioxidant, antibacterial, and anticancer activities.…”

A preliminary study on preparation, characterization, and prebiotic activity of a polysaccharide from the edible mushroom Ramaria flava

“…Although a huge difference lies in carbohydrate compositions between DRFP, inulin, and pistachio hull water‐soluble polysaccharides (PHP), they all would enter the colon without much degradation occurring (Akbari‐Alavijeh et al, 2018). The β‐type glycosidic bonds with a more stable characteristic than α‐linkages might be the underlying reason for the high degree of nondigestibility (Chen et al, 2020). Considering the high stability of DRFP to resist enzyme digestion, we hazarded a conjecture that DRFP is a good prebiotic candidate like inulin and PHP.…”

“…This was coincident with the findings of Akbari-Alavijeh et al (2018), who explained that inulin was more chemically unstable in an acid environment and possessed a higher hydrolyzing degree in gastric juice. However The β-type glycosidic bonds with a more stable characteristic than αlinkages might be the underlying reason for the high degree of nondigestibility (Chen et al, 2020). Considering the high stability of DRFP to resist enzyme digestion, we hazarded a conjecture that DRFP is a good prebiotic candidate like inulin and PHP.…”

Immunomodulatory Effect of Sasa quelpaertensis Leaves Fermentation Products in Mice

“…Among the components constituting Sasa quelpaertensis, there are many similar functional components with immune enhancing activity. Among the components exhibiting immune enhancing function, the active effect has been reported in mixtures containing polysaccharide [10,11], p-coumaric acid [12], and Sasa quelpaertensis [13]. However, their various beneficial effects have not been fully evaluated.…”