Comparisons of protective effects between two sea cucumber hydrolysates against diet induced hyperuricemia and renal inflammation in mice

Wan, Haitao; Han, Jiaojiao; Tang, Shu; Bao, Wei; Lu, Chenyang; Zhou, Jun; Ming, Tinghong; Li, Ye; Su, Xiurong

doi:10.1039/c9fo02425e

Cited by 76 publications

(86 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Edible protein-derived oligopeptides are attractive candidates owing to their beneficial effects on hyperuricemia and their safety in rodent models. [20,21] In the present study, AJOP, with known composition, was prepared from A. japonicus (Table S2, Supporting Information) [7] ). We found that a relatively low dose of AJOP significantly alleviated hyperuricemia, renal injury, and systemic inflammation, without evident side effects (Figures 1 and 2).…”

Section: Discussionmentioning

confidence: 99%

“…[11] In our previous study on the anti-hyperuricemic effects of AJOP, the fecal microbiota was analyzed, and differences among the control, model, and treatment groups were observed. [7] However, different environmental factors and functions of GIT segments lead to different microbiota profiles. [12,13] The limited measurement of fecal microbiota cannot provide comprehensive information on the changes in the microbiota during AJOP treatment and is not sufficient to clarify the anti-hyperuricemic effects of AJOP on microbiota modulation.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Apostichopus japonicus Oligopeptide Induced Heterogeneity in the Gastrointestinal Tract Microbiota and Alleviated Hyperuricemia in a Microbiota‐Dependent Manner

Tang

Han

et al. 2021

Molecular Nutrition Food Res

Self Cite

View full text Add to dashboard Cite

Scope: This study aims to investigate the protective effect of Apostichopus japonicus oligopeptide (AJOP) on hyperuricemia, demonstrate the modulation of the gastrointestinal tract (GIT) microbiota, and clarify the underlying microbiota-dependent mechanism. Methods and Results: Hyperuricemic mice treated with AJOP and subjected to corresponding fecal microbiota transplantation (FMT) are used to observe the beneficial effects of AJOP and microbiota. Gene transcriptions are measured using quantitative real-time PCR. The GIT (stomach, colon, cecum, and feces) microbiota is analyzed by 16S rDNA sequencing and the short-chain fatty acids are detected using GC-MS. Dietary administration of AJOP significantly alleviates hyperuricemia, regulates uric acid metabolism, inhibites the activation of the NLRP3 inflammasome and NF-B-related signaling pathway, and restores m6A methylation levels. In addition, substantial heterogeneity is observed in GIT microbiota. Furthermore, FMT effectively alleviates hyperuricemia in mice by selectively regulating the corresponding pathways associated with AJOP treatment, indicating that the mechanism underlying the protective effects of AJOP is partly microbiota-dependent. Conclusion: This study demonstrates that AJOP exerts a protective effect on hyperuricemic mice by regulating uric acid metabolism, resulting in substantial heterogeneity among the GIT microbiota, thus mediating the beneficial effects in a microbiota-dependent manner.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Apostichopus japonicus Oligopeptide Induced Heterogeneity in the Gastrointestinal Tract Microbiota and Alleviated Hyperuricemia in a Microbiota‐Dependent Manner

Tang

Han

et al. 2021

Molecular Nutrition Food Res

Self Cite

View full text Add to dashboard Cite

show abstract

“…Following that, the mice are given isoflurane anesthesia, blood samples are collected from the eyelids, and serum is isolated by centrifugation for 15 min at 4 °C and 3000 rpm, and then stored at 80 °C for biochemical studies. The mice are sacrificed through cervical dislocation after sample processing, and their visceral organs are excised, weighed, and preserved at 80 °C before further study [ 41 ].…”

Section: Experimental In-vivo Models For Goutmentioning

confidence: 99%

A brief review on in vivo models for Gouty Arthritis

Patil¹,

Soni²,

Acharya³

2021

Metabolism Open

View full text Add to dashboard Cite

Gout is more common in men than in women, by a factor of 3.1–10.1. Gout prevalence and incidence have increased in recent decades, with prevalence reaching 11–13% and incidence reaching 0.4% in people over the age of 80. Age-related renal impairment, altered drug distribution, and increased prevalence of comorbidities have significant consequences for safe and effective gout pharmacotherapy. The Discovery of Fruitful in-vivo animal models needs the effective screening of drugs or formulations used in the treatment of gout. In vivo animal models of Gouty arthritis are extensively used to investigate pathogenic mechanisms governing inflammation-driven bone and cartilage damage. Four commonly utilized models include the Potassium oxonate induced hyperuricemic model, MSU crystals induced gouty arthritis animal model, Animal Model of Acute Gouty Arthritis with Hyperuricemia, and Diet-induced hyperuricemia. These offer unique advantages for correlating different aspects of gouty arthritis with human disease. In-vivo animal models served as testing beds for novel biological therapies, including cytokine blockers and small molecule inhibitors of intracellular signaling that have revolutionized gouty arthritis treatment. This review highlights a brief overview of in vivo experimental models for assessment of hypouricemic, anti-inflammatory, as well as renal protective effects of test compounds with some evaluation parameters in detail.

show abstract

“…Enzymatic hydrolysates of A. japonicus (EH‐JAP) and A. leucoprocta (EH‐LEU) could also inhibit the uric acid biosynthesis and promote uric acid excretion. These hydrolysates could downregulate the transcription of pro‐inflammatory cytokines, upregulate the transcription of anti‐inflammatory cytokines, and inhibit the activation of the Toll‐like receptor 4/myeloid differentiation factor 88/NF‐κB signaling pathway in a diet‐induced hyperuricemia mouse model (H. Wan et al., 2020). Kidney bean ( Phaseolus vulgaris L .)…”

Section: Introductionmentioning

confidence: 99%

Purification, identification, and computational analysis of xanthine oxidase inhibitory peptides from kidney bean

Hou

2021

Journal of Food Science

View full text Add to dashboard Cite

Hyperuricemia is related to plenty of diseases, seriously damaging human health. Current clinical drugs used to treat hyperuricemia have many adverse effects. In this study, kidney bean hydrolysate (KBH) was found to exert high xanthine oxidase inhibitory (XOI) activity. Compared to KBH (50.31 ± 2.73%), XOI activities of three fractions (Mw <5 kDa, Mw<3 kDa, Mw < 1 kDa) by ultrafiltration were higher and increased to 58.58 ± 3.57%, 59.34 ± 1.78%, and 55.05 ± 5.00%, respectively (P < 0.05). A total of 69 peptides were identified by HPLC‐ESI‐MS/MS and analyzed binding affinities with XO with the help of molecular docking. AVDSLVPIGR, DWYDIK, LDNLLR, ISPIPVLK, ISSLEMTR showed well binding affinities with XO and DWYDIK presented the highest XOI activity (68.63 ± 5.07%) among five synthetic peptides (P < 0.05). Additionally, visual analysis results indicated that DWYDIK was pushed into the hydrophobic channel and formed hydrogen bonds with pivotal amino acids of xanthine oxidase. Overall, KBH could be a promising candidate as anti‐ hyperuricemia functional food. Practical Application This research initially revealed that kidney bean peptides could significantly inhibit the activity of xanthine oxidase, indicating kidney bean peptides could be a treatment for hyperuricemia. Kidney bean peptides may have commercial potentials as a safer alternative with few side effects to drugs.

show abstract

Comparisons of protective effects between two sea cucumber hydrolysates against diet induced hyperuricemia and renal inflammation in mice

Abstract: Differences in the anti-hyperuricemic and anti-inflammation effects between two sea cucumber hydrolysates in diet induced hyperuricemic mice.

Cited by 76 publications

References 49 publications

Apostichopus japonicus Oligopeptide Induced Heterogeneity in the Gastrointestinal Tract Microbiota and Alleviated Hyperuricemia in a Microbiota‐Dependent Manner

Apostichopus japonicus Oligopeptide Induced Heterogeneity in the Gastrointestinal Tract Microbiota and Alleviated Hyperuricemia in a Microbiota‐Dependent Manner

A brief review on in vivo models for Gouty Arthritis

Purification, identification, and computational analysis of xanthine oxidase inhibitory peptides from kidney bean

Contact Info

Product

Resources

About