Comparisons of the Effects of Vibration of Two Centrifugal Systems on the Growth and Morphological Parameters of the Moss <i>Physcomitrella patens</i>

Abstract

As pioneer plants mosses are adaptable to extreme environments and are the first to colonize previously uncolonized land. Recently, we designed two centrifuges, MIJ-17 and MK-3, to investigate the effects of long-term cultivation under moderate hypergravity conditions on the growth of mosses. We examined the effects of the vibration generated by these centrifuges on the growth and morphological parameters of the moss Physcomitrella patens, because plants are generally sensitive to vibration. In MIJ-17, random vibration was detected by a micro threeaxis acceleration sensor during centrifugation at 2, 5, and 10 G, whereas vibration was negligible in MK-3. Therefore, we compared the growth and morphological parameters of P. patens gametophores cultivated with MIJ-17 and MK-3 at 10 G. The vibration generated by MIJ-17 did not significantly affect the growth and morphological parameters of P. patens gametophores. Thus, we conclude that the vibration of MIJ-17 has a negligible impact on the growth of P. patens gametophores.©2017

Prolonged exposure to hypergravity increases biomass and alters biomass allocation inArabidopsis thaliana(L.) Heynh. with no apparent impact on element content in the shoot system

“…Bruch et Schimp were significantly increased under 10 g and the area-based photosynthesis rate was also enhanced under 10 g (Takemura et al, 2017; Takemura et al, 2017). However, the first custom-built centrifuge equipped with a lighting system, MIJ-7, has a slight problem of vibration generated during centrifugation (Mori et al, 2017). Therefore, the authors have further developed custom-built centrifuges having lighting systems with only negligible vibration, MK-2 and MK-3, which is more suitable for longer period of hypergravity cultivation with seed plants (Mori et al, 2017; Takemura et al, 2017).…”