Compatibility of
            <i>Achyranthes bidentata</i>
            components in reducing inflammatory response through Arachidonic acid pathway for treatment of Osteoarthritis

Li, Zanzhu; Ma, Dujun; Peng, Liping; Li, Yuan; Liao, Zhouwei; Yu, Tian

doi:10.1080/21655979.2021.2020394

Cited by 11 publications

(9 citation statements)

References 36 publications

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“…Arachidonic acid metabolites were able to inhibit cell proliferation in the inflamed synovium, thereby limiting arthritis inflammation and pannus formation. 50 , 51 The enrichment of GPXs in the arachidonic acid metabolic pathway suggested that GPXs may reduce the inflammatory response to OA treatment through the arachidonic acid pathway.…”

Section: Discussionmentioning

confidence: 99%

Biological Functions of Selenoprotein Glutathione Peroxidases (GPXs) and their Expression in Osteoarthritis

Zhao¹,

Tang²,

Zhang³

et al. 2023

JIR

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Purpose In order to further study the biological functions of glutathione peroxidases (GPXs) and their expression level in patients with osteoarthritis (OA), we fully explored the potential relationship between GPXs and OA. This will provide new ideas for basic biological studies and therapeutic strategies for OA patients. Patients and Methods In this study, bioinformatics techniques were used to explore the biological functions of five GPXs. The core genes related to the biological functions of GPXs were identified by constructing a protein-protein interaction network (PPI). In addition, we utilized microarray data in public databases to analyze the expression levels of GPXs in OA patients and healthy controls. Finally, we used quantitative real-time polymerase chain reaction (qRT-PCR) to detect the expression of GPXs in OA patients and controls to validate our bioinformatic analysis results. Results Enrichment analysis showed GPXs were mainly enriched in the glutathione metabolic pathway and participate in the biological process of oxidative stress response, and further play an antioxidant role. The PPI network indicated that superoxide dismutase 1 (SOD1), superoxide dismutase 2(SOD2) and catalase (CAT) were the core proteins of this network. GPX1 was regulated by the greatest number of miRNAs. Experiments showed that the expression of GPX1 was elevated in OA patients compared with controls. Conclusion GPXs play an important antioxidant role in oxidative stress response. The expression of GPX1 was elevated in peripheral blood mononuclear cells (PBMCs) of OA patients. The changes of GPXs in OA patients may regulate the level of oxidative stress, which may influence synovial lesions and chondrocyte apoptosis.

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Section: Discussionmentioning

confidence: 99%

Biological Functions of Selenoprotein Glutathione Peroxidases (GPXs) and their Expression in Osteoarthritis

Zhao¹,

Tang²,

Zhang³

et al. 2023

JIR

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“…The staining procedure for hematoxylin and eosin (H&E) was performed following the established protocol described in previous publications [ 16 ]. Briefly, ovarian tissues fixed with 4% paraformaldehyde (Servicebio, China) were dehydrated, embedded, and sectioned (The maximum transverse section of the ovary wasserially sliced into 4 μm sections).…”

Section: Methodsmentioning

confidence: 99%

Human mesenchymal stem cells derived exosomes improve ovarian function in chemotherapy-induced premature ovarian insufficiency mice by inhibiting ferroptosis through Nrf2/GPX4 pathway

Zhou,

Huang,

Zeng

et al. 2024

J Ovarian Res

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Background Chemotherapy exposure has become a main cause of premature ovarian insufficiency (POI). This study aimed to evaluate the role and molecular mechanism of human umbilical cord mesenchymal stem cell-derived exosomes (hUMSC-Exos) in ovarian function protection after chemotherapy. Methods hUMSC-Exos were applied to cyclophosphamide-induced premature ovarian insufficiency mice and human ovarian granulosa tumor cells (KGN) to determine their effects on follicular development and granulosa cell apoptosis. Evaluation was done for iron ion and reactive oxygen species (ROS) production, lipid peroxidation levels, and changes in iron death-related molecules (nuclear factor (erythroid-derived 2)-like 2 (Nrf2), Glutathione Peroxidase enzyme 4 (GPX4), and Solute carrier family 7 member 11 cystine glutamate transporter (SLC7A11; xCT)). Furthermore, rescue experiments using an Nrf2 inhibitor were performed to assess the therapeutic effects of hUMSC-Exos on granulosa cells. Results hUMSC-Exos promoted ovarian hormone levels and primary follicle development in POI mice and reduced granulosa cell apoptosis. After hUMSC-Exos treatment, the ROS production, free iron ions and lipid peroxidation levels of granulosa cells decreased, and the iron death marker proteins Nrf2, xCT and GPX4 also decreased. Furthermore, the Nrf2 inhibitor ML385 significantly attenuated the effects of hUMSC-Exos on granulosa cells. Conclusion hUMSC-Exos inhibit ferroptosis and protect against CTX-induced ovarian damage and granulosa cell apoptosis through the Nrf2/GPX4 signaling pathway, revealing a novel mechanism of hUMSC-Exos in POI therapy.

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“…From week 6 after modeling, the normal and model groups were given normal saline at a dose of 10 ml/(kg·day), whereas the ABPS group was given a clinically equivalent dose of ABPS at 400 mg/(kg·day) ( 20 ). Almost all previous studies opted for the oral intake method for ABPS for treating bone diseases, such as osteoporosis ( 12 , 20 , 21 ) and OA ( 13 ). Since the present study was focused on exploring the protective mechanism of ABPS against OA, the most common delivery method, which is the oral gavage, was adopted for the rats.…”

Section: Methodsmentioning

confidence: 99%

“…The chemical structure of ABPS is dominated by furan rings, where its molecular weight is in the range of 1.4-3.4 kDa ( 11 ). ABPS has been widely applied for the treatment of bone-related diseases, such as osteoporosis and OA, due to its observed protective effects on the bone ( 12 , 13 ). As an anti-inflammatory agent, ABPS has been previously shown to interfere with the inflammatory activation that occurs during OA and the metabolic pathway of arachidonic acid ( 13 ).…”

Section: Introductionmentioning

confidence: 99%

“…ABPS has been widely applied for the treatment of bone-related diseases, such as osteoporosis and OA, due to its observed protective effects on the bone ( 12 , 13 ). As an anti-inflammatory agent, ABPS has been previously shown to interfere with the inflammatory activation that occurs during OA and the metabolic pathway of arachidonic acid ( 13 ). In a castrated rat model of osteoporosis, ABPS has been documented to significantly increase the bone-mineral content and biomechanical properties of the femur, which protected these rats from osteoporosis by stimulating bone formation ( 14 ).…”

Section: Introductionmentioning

confidence: 99%

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Protective role of Achyranthes bidentata polysaccharides against chondrocyte extracellular matrix degeneration through lncRNA GAS5 in osteoarthritis

Qiu

Huang

et al. 2022

Exp Ther Med

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Achyranthes bidentata polysaccharides (ABPS) is an active ingredient of the flowering plant Achyranthes bidentata that has been previously reported to be effective for the treatment of osteoarthritis (OA). However, the underlying molecular mechanism remain to be fully clarified. Emerging studies have shown that the long non-coding RNA (lncRNA) growth arrest-specific transcript 5 (GAS5) is involved in the pathogenesis of OA. Therefore, the present study aimed to investigate the potential mechanism of ABPS by focusing on its effects on the regulation of chondrocyte extracellular matrix (ECM) homeostasis, with particular emphasis on lncRNA GAS5. In the present study, the modified Hulth method was used to construct OA rats, which were gavaged with 400 mg/kg ABPS for 8 weeks. Histopathological changes in cartilage and subchondral bone were evaluated by hematoxylin-eosin staining and Safranin O-fast green staining. In in vitro experiments, IL-1β-treated chondrocytes were infected with Lenti-lncRNA GAS5. Fluorescence in situ hybridization assay was performed to measure the expression of the lncRNA GAS5 in chondrocytes. Moreover, the relative expression level of lncRNA GAS5 in cartilage tissue and chondrocytes was detected using reverse transcription-quantitative PCR. Western blot analysis was used to detect protein expression levels of MMP-9, MMP-13, TIMP-1, TIMP-3 and type II collagen in cartilage tissue and chondrocytes. The results indicated that ABPS delayed the degradation of the ECM by chondrocytes in addition to reducing lncRNA GAS5 expression both in vivo and in vitro. Furthermore, silencing of lncRNA GAS5 expression in IL-1β-treated chondrocytes downregulated the protein expression of MMP-9 and MMP-13 whilst upregulating the expression of tissue inhibitor matrix metalloproteinase (TIMP)-1, TIMP-3 and type II collagen. To conclude, the present study provides evidence that ABPS can inhibit the expression of lncRNA GAS5 in chondrocytes to regulate the homeostasis of ECM, which in turn may delay the occurrence of cartilage degeneration during OA.

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Compatibility of Achyranthes bidentata components in reducing inflammatory response through Arachidonic acid pathway for treatment of Osteoarthritis

Cited by 11 publications

References 36 publications

Biological Functions of Selenoprotein Glutathione Peroxidases (GPXs) and their Expression in Osteoarthritis

Biological Functions of Selenoprotein Glutathione Peroxidases (GPXs) and their Expression in Osteoarthritis

Human mesenchymal stem cells derived exosomes improve ovarian function in chemotherapy-induced premature ovarian insufficiency mice by inhibiting ferroptosis through Nrf2/GPX4 pathway

Protective role of Achyranthes bidentata polysaccharides against chondrocyte extracellular matrix degeneration through lncRNA GAS5 in osteoarthritis

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