Compensatory Movement of Contralateral Vocal Folds in Patients With Unilateral Vocal Fold Paralysis

Xu, Xiufeng; Zhuang, Pinghui; Wilson, Azure; Jiang, Jack J.

doi:10.1016/j.jvoice.2019.09.010

Cited by 6 publications

(3 citation statements)

References 14 publications

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“…Postmortem harvested larynges were measured three‐dimensionally with quantitative ultrasound (US) to gauge MEE injection effect on laryngeal adductor volume following RLN injury. We detected reduced relative adductor muscle volume following denervation, consistent with expected muscle atrophy and contralateral compensation 36,37 . (Fig.…”

Section: Discussionsupporting

confidence: 84%

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Early Changes in Porcine Larynges Following Injection of Motor‐Endplate Expressing Muscle Cells for the Treatment of Unilateral Vocal Fold Paralysis

et al. 2023

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ObjectivesNo curative injectable therapy exists for unilateral vocal fold paralysis. Herein, we explore the early implications of muscle‐derived motor‐endplate expressing cells (MEEs) for injectable vocal fold medialization after recurrent laryngeal nerve (RLN) injury.MethodsYucatan minipigs underwent right RLN transection (without repair) and muscle biopsies. Autologous muscle progenitor cells were isolated, cultured, differentiated, and induced to form MEEs. Three weeks after the injury, MEEs or saline were injected into the paralyzed right vocal fold. Outcomes including evoked laryngeal electromyography (LEMG), laryngeal adductor pressure, and acoustic vocalization data were analyzed up to 7 weeks post‐injury. Harvested porcine larynges were examined for volume, gene expression, and histology.ResultsMEE injections were tolerated well, with all pigs demonstrating continued weight gain. Blinded analysis of videolaryngoscopy post‐injection revealed infraglottic fullness, and no inflammatory changes. Four weeks after injection, LEMG revealed on average higher right distal RLN activity retention in MEE pigs. MEE‐injected pigs on average had vocalization durations, frequencies, and intensities higher than saline pigs. Post‐mortem, the MEE‐injected larynges revealed statistically greater volume on quantitative 3D ultrasound, and statistically increased expression of neurotrophic factors (BDNF, NGF, NTF3, NTF4, NTN1) on quantitative PCR.ConclusionsMinimally invasive MEE injection appears to establish an early molecular and microenvironmental framework to encourage innate RLN regeneration. Longer follow‐up is needed to determine if early findings will translate into functional contraction.Level of EvidenceNA Laryngoscope, 2023

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Section: Discussionsupporting

confidence: 84%

“…We detected reduced relative adductor muscle volume following denervation, consistent with expected muscle atrophy and contralateral compensation. 36,37 (Fig. 5B).…”

Section: Post-injection Outcomesmentioning

confidence: 94%

Early Changes in Porcine Larynges Following Injection of Motor‐Endplate Expressing Muscle Cells for the Treatment of Unilateral Vocal Fold Paralysis

et al. 2023

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“…Interestingly, we also observed a compensatory movements of vocal folds on the healthy side 3 weeks after AAV treatment. According to publication, the average time of compensatory movements of vocal folds was 7.03 ± 6.05 months from onset 34 . These results suggest that suppression of MuRF‐1 expression is probably an effective approach for the treatment of early‐stage internal laryngeal muscle denervated atrophy.…”

Section: Discussionmentioning

confidence: 89%

MuRF‐1 is Involved in Laryngeal Muscle Denervation Atrophy by Regulating G‐Actin Ubiquitination

Wang,

et al. 2023

The Laryngoscope

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ObjectiveMuscle RING‐finger protein‐1 (MuRF‐1), an E3 ubiquitin ligase, has been reported to aggravate skeletal muscle denervated atrophy by mediating the ubiquitination degradation of multiple proteins, whereas the molecular mechanism underlying MuRF‐1‐mediated internal laryngeal muscle denervated atrophy remains unknown.MethodsA rat unilateral recurrent laryngeal nerve (RLN) transection model was established to evaluate denervated muscle atrophy of the larynx. The expression of MuRF‐1, G‐ and F‐actin in thyroarytenoid muscle (TA) myocytes before and after RLN injury was analyzed by immunofluorescence and Western blotting. Coimmunoprecipitation experiments detected molecular interactions between MuRF‐1 and G‐actin. Immunoprecipitation tested MuRF‐1‐mediated ubiquitination of G‐actin in denervated and innervated TA muscle tissues. The shRNA‐MuRF‐1 AAV was used to suppress MuRF‐1 expression in denervated TA muscles in vivo.ResultsFirst, MuRF‐1 expression was significantly elevated in denervated TA muscle compared to innervated TA muscle (p < 0.001). Second, there was a progressive increase in the G/F‐actin ratio in TA myocytes from day 3 to 14 after RLNI (p < 0.01). Furthermore, colocalization of MuRF‐1 and G‐actin in denervated TA myocytes was observed. Moreover, the upregulation of MuRF‐1 was closely associated with the ubiquitination of G‐actin in denervated TA myocytes and muscle tissues. Knockdown of MuRF‐1 decelerated the degree of TA muscle atrophy compared with that in the Blank and NC groups (p < 0.001) but seemed to promote the compensatory movement of the healthy side.ConclusionCollectively, we illustrate a novel molecular mechanism underlying MuRF‐1‐mediated internal laryngeal muscle denervated atrophy in that MuRF‐1 could promote disequilibrium of the G/F‐actin ratio by regulating G‐actin ubiquitination.Level of EvidenceN/A Laryngoscope, 2023

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Application of 4D-CT Scanning in Differential Diagnosis of Arytenoid Subluxation and Vocal Fold Paralysis

Wang

et al. 2022

Journal of Voice

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Compensatory Movement of Contralateral Vocal Folds in Patients With Unilateral Vocal Fold Paralysis

Cited by 6 publications

References 14 publications

Early Changes in Porcine Larynges Following Injection of Motor‐Endplate Expressing Muscle Cells for the Treatment of Unilateral Vocal Fold Paralysis

Early Changes in Porcine Larynges Following Injection of Motor‐Endplate Expressing Muscle Cells for the Treatment of Unilateral Vocal Fold Paralysis

MuRF‐1 is Involved in Laryngeal Muscle Denervation Atrophy by Regulating G‐Actin Ubiquitination

Application of 4D-CT Scanning in Differential Diagnosis of Arytenoid Subluxation and Vocal Fold Paralysis

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