Competition and coexistence of aerobic ammonium- and nitrite-oxidizing bacteria at low oxygen concentrations

Sliekers, Olav; Haaijer, Suzanne Caroline Marianne; Stafsnes, Marit Hallvardsdotter; Kuenen, J.G.; Jetten, Mike S. M.

doi:10.1007/s00253-005-1974-6

Cited by 137 publications

(75 citation statements)

References 67 publications

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“…One possible explanation of the presence of AOB is that some AOB can reduce nitrite to N 2 O or NO gases under oxygen-limiting conditions (27,28). Another possible reason is that since nitrification perhaps occurred in the inlet zone (up to P2) of the reactor because a trace amount of O 2 was present in the medium (Ͻ0.8 mg/liter) (37), nitrifying bacteria present in the P1 biofilm could detach and then reattach to the biofilms or accumulate downstream in the reactor. In addition, for AOB, the FISH signal intensity (i.e., ribosome content) does not decrease significantly during periods of starvation.…”

Section: Vol 73 2007 In Situ Structure and Function Of Anammox Biofmentioning

confidence: 99%

In Situ Activity and Spatial Organization of Anaerobic Ammonium-Oxidizing (Anammox) Bacteria in Biofilms

Kindaichi

Tsushima

Ogasawara

et al. 2007

Appl Environ Microbiol

147

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We investigated autotrophic anaerobic ammonium-oxidizing (anammox) biofilms for their spatial organization, community composition, and in situ activities by using molecular biological techniques combined with microelectrodes. Results of phylogenetic analysis and fluorescence in situ hybridization (FISH) revealed that "Brocadia"-like anammox bacteria that hybridized with the Amx820 probe dominated, with 60 to 92% of total bacteria in the upper part (<1,000 m) of the biofilm, where high anammox activity was mainly detected with microelectrodes. The relative abundance of anammox bacteria decreased along the flow direction of the reactor. FISH results also indicated that Nitrosomonas-, Nitrosospira-, and Nitrosococcus-like aerobic ammonia-oxidizing bacteria (AOB) and Nitrospira-like nitrite-oxidizing bacteria (NOB) coexisted with anammox bacteria and accounted for 13 to 21% of total bacteria in the biofilms. Microelectrode measurements at three points along the anammox reactor revealed that the NH 4 ؉ and NO 2 ؊ consumption rates decreased from 0.68 and 0.64 mol cm ؊2 h ؊1 at P2 (the second port, 170 mm from the inlet port) to 0.30 and 0.35 mol cm ؊2 h ؊1 at P3 (the third port, 205 mm from the inlet port), respectively. No anammox activity was detected at P4 (the fourth port, 240 mm from the inlet port), even though sufficient amounts of NH 4 ؉ and NO 2 ؊ and a high abundance of anammox bacteria were still present. This result could be explained by the inhibitory effect of organic compounds derived from biomass decay and/or produced by anammox and coexisting bacteria in the upper parts of the biofilm and in the upstream part of the reactor. The anammox activities in the biofilm determined by microelectrodes reflected the overall reactor performance. The several groups of aerobic AOB lineages, Nitrospira-like NOB, and Betaproteobacteria coexisting in the anammox biofilm might consume a trace amount of O 2 or organic compounds, which consequently established suitable microenvironments for anammox bacteria.Anaerobic ammonium oxidation (anammox) is a microbiological oxidation of ammonium, with nitrite as the electron acceptor and dinitrogen gas as the main product, and is mediated by a group of deep-branching Planctomycete-like bacteria (12, 39). Anammox bacteria have been detected in different wastewater treatment facilities and environments in the world (for a review, see reference 34). Currently, four genera of anammox bacteria have been reported and named, including Brocadia, Kuenenia, Scalindua, and Anammoxoglobus (14,34).The anammox process is a new and promising alternative to conventional nitrogen removal processes. A better understanding of the ecophysiology (i.e., microbial community structure and in situ activity) of anammox bacteria in complex biofilms is essential for implementing the anammox process as a manageable and reliable nitrogen removal process in wastewater treatment. However, related information is limited because anammox bacteria are strict anaerobic autotrophs and thus have not yet been isolate...

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Section: Vol 73 2007 In Situ Structure and Function Of Anammox Biofmentioning

confidence: 99%

In Situ Activity and Spatial Organization of Anaerobic Ammonium-Oxidizing (Anammox) Bacteria in Biofilms

Kindaichi

Tsushima

Ogasawara

et al. 2007

Appl Environ Microbiol

147

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“…ENI11 (99%) Wastewater from the fertilizer industry contained very high levels of ammonical nitrogen and negligible amounts of organic carbon owing to which nitrifying microbial community were employed for its treatment. A syntrophic association is known to exist between AOB and autotrophic nitrite oxidizing bacteria (NOB) and they can therefore coexist in biofilms or in aggregates in wastewater treatment plants (Schramm et al, 1999;Sliekers et al, 2005;Siripong and Rittmann, 2007). AOB are described to be present in the outer regions of the biofilms or aggregates and provide nitrite (nitrogen source) to NOB residing in their close vicinity in the inner portion of the biofilms or aggregates (Schramm et al, 1999).…”

Section: Removal Of Ammonia Frommentioning

confidence: 99%

“…In the presence of low oxygen concentrations, co-cultures of AOB and NOB is possible but levels of ammonia in the system should also be low (Sliekers et al, 2005). In the presence of high ammonia and low oxygen concentrations, NOB would be washed out of the reactors and ammonium would be converted to mainly nitrite, nitric oxide and nitrous oxide by Nitrosomonas sp.…”

Section: Ammonium Removal From Fertilizer Industry Effluentmentioning

confidence: 99%

“…In the presence of high ammonia and low oxygen concentrations, NOB would be washed out of the reactors and ammonium would be converted to mainly nitrite, nitric oxide and nitrous oxide by Nitrosomonas sp. and other related bacteria (Sliekers et al, 2005;Keluskar et al, 2013). Ammonia levels were exceedingly high in the fertilizer effluent used in the present study, hence for simultaneous growth of AOB and NOB under identical conditions in a single reactor, oxygen concentration was also kept high to allow stable nitrification to occur in the system.…”

Section: Ammonium Removal From Fertilizer Industry Effluentmentioning

confidence: 99%

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Bioremediation of High Strength Ammonia Laden Fertilizer Industry Effluent with Extremely Low C: N Ratio Using Simultaneous Aerobic Nitrification and Denitrification

Keluskar¹

2017

Int.J.Curr.Microbiol.App.Sci

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“…Both would significantly reduce energy consumption for nitrate production and for providing an organic electron donor source for denitrification. Most approaches toward the achievement of partial nitrification have been reported within recent decades (Guo et al, 2010;Kim et al, 2005;Sliekers et al, 2005). Due to the higher growth rate of AOB than NOB (Munz, 2011), maintaining the predominance of AOB over NOB is one of the key factors for achieving successful partial nitrification.…”

Section: Introductionmentioning

confidence: 99%

Sustainable nitrification in fluidised bed reactor with immobilised sludge pellets

Wang¹,

Zhou²,

Li³

et al. 2013

WSA

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Sustainable immobilised microbial pellets were developed with water-borne polyurethane (WPU) material together with powdered activated carbon (PAC) and activated sludge as microbial inoculums for nitrification or partial nitrification. The nitrification performance and the influencing factors were studied with lab-scale aerobic fluidised bed reactors (FBR) under various temperature conditions. During the start-up period, quickly increasing the influent ammonium concentration from 40 to 320 mg N·ℓ -1 led to a stable nitrification performance with high nitrite accumulation (>80%). Characterisation of the FBR performance indicated that the desired partial nitrification could be achieved at pH 7.8-8.5, dissolved oxygen (DO) 3-5 mg·ℓ -1 and temperature between 24 and 29°C. Addition of organic carbon (glucose) improved the ammonium removal but decreased the nitrite accumulation ratio significantly. TOC concentration above 800 mg·ℓ -1 was not able to cause the inhibition of the heterotrophs over the nitrifiers. PCR-DGGE results indicated the presence of Nitrosomonas (ammonia-oxidising bacteria) and Nitrobacter (nitrite-oxidising bacteria) in the immobilised pellets.

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Competition and coexistence of aerobic ammonium- and nitrite-oxidizing bacteria at low oxygen concentrations

Cited by 137 publications

References 67 publications

In Situ Activity and Spatial Organization of Anaerobic Ammonium-Oxidizing (Anammox) Bacteria in Biofilms

In Situ Activity and Spatial Organization of Anaerobic Ammonium-Oxidizing (Anammox) Bacteria in Biofilms

Bioremediation of High Strength Ammonia Laden Fertilizer Industry Effluent with Extremely Low C: N Ratio Using Simultaneous Aerobic Nitrification and Denitrification

Sustainable nitrification in fluidised bed reactor with immobilised sludge pellets

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