Competition between polyphosphate- and glycogen-accumulating organisms in biological phosphorus removal systems – effect of temperature

Whang, Liang Ming; Park, J. K.

doi:10.2166/wst.2002.0476

Cited by 53 publications

(32 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Not all explanations for such events allow for any role of microbes in process failure (Schönborn et al, 2001) and many that do usually lack any convincing supporting microbiological evidence (Filipe et al, 2001;Whang & Park 2002). Cech & Hartman (1993) noticed large numbers of cocci in distinctive tetrads, a morphotype they called the 'G-bacteria', in a reactor showing poor EBPR capacity that was fed glucose.…”

Section: Introductionmentioning

confidence: 99%

Are some putative glycogen accumulating organisms (GAO) in anaerobic : aerobic activated sludge systems members of the α-Proteobacteria?

2004

View full text Add to dashboard Cite

Activated sludge plants designed to remove phosphorus microbiologically often perform unreliably. One suggestion is that the polyphosphate-accumulating organisms (PAO) are out-competed for substrates by another group of bacteria, the glycogen-accumulating organisms (GAO) in the anaerobic zones of these processes. This study used fluorescence in situ hybridization (FISH) and denaturing gradient gel electrophoresis (DGGE) to analyse the communities from laboratory-scale anaerobic : aerobic sequencing batch reactors. Members of the genus Sphingomonas in the a-Proteobacteria were present in large numbers in communities with poor phosphorus removal capacity where the biomass had a high glycogen content. Their ability to store poly-b-hydroxyalkanoates anaerobically, but not aerobically, and not accumulate polyphosphate aerobically is consistent with these organisms behaving as GAO there. No evidence was found to support an important role for the c-Proteobacteria as possible GAO in these communities, although these bacterial populations have been considered in other studies to act as possible competitors for the PAO.

show abstract

Section: Introductionmentioning

confidence: 99%

Are some putative glycogen accumulating organisms (GAO) in anaerobic : aerobic activated sludge systems members of the α-Proteobacteria?

2004

View full text Add to dashboard Cite

show abstract

“…Sequential anaerobic and aerobic phases are employed for the enrichment of a group of bacteria known as polyphosphate-accumulating organisms (PAOs), which are primarily responsible for EBPR. Another group of bacteria, glycogen-accumulating organisms (GAOs), also proliferate in systems with sequential anaerobic and aerobic phases, competing with PAOs for the often limiting carbon substrates (Bond et al, 1998;Satoh et al, 1994;Thomas et al, 2003;Whang & Park, 2002). Currently, there are no pure cultures of PAOs or GAOs (Seviour et al, 2003), so their biochemical transformations have been determined through chemical analyses of enriched laboratory-scale mixed EBPR microbial communities.…”

Section: Introductionmentioning

confidence: 99%

Anaerobic and aerobic metabolism of glycogen-accumulating organisms selected with propionate as the sole carbon source

et al. 2006

View full text Add to dashboard Cite

In the microbial competition observed in enhanced biological phosphorus removal (EBPR) systems, an undesirable group of micro-organisms known as glycogen-accumulating organisms (GAOs) compete for carbon in the anaerobic period with the desired polyphosphate-accumulating organisms (PAOs). Some studies have suggested that a propionate carbon source provides PAOs with a competitive advantage over GAOs in EBPR systems; however, the metabolism of GAOs with this carbon source has not been previously investigated. In this study, GAOs were enriched in a laboratory-scale bioreactor with propionate as the sole carbon source, in an effort to better understand their biochemical processes. Based on comprehensive solid-, liquid-and gas-phase chemical analytical data from the bioreactor, a metabolic model was proposed for the metabolism of propionate by GAOs. The model adequately described the anaerobic stoichiometry observed through chemical analysis, and can be a valuable tool for further investigation of the competition between PAOs and GAOs, and for the optimization of the EBPR process. A group of Alphaproteobacteria dominated the biomass (96 % of Bacteria) from this bioreactor, while postfluorescence in situ hybridization (FISH) chemical staining confirmed that these Alphaproteobacteria produced poly-b-hydroxyalkanoates (PHAs) anaerobically and utilized them aerobically, demonstrating that they were putative GAOs. Some of the Alphaproteobacteria were related to Defluvicoccus vanus (16 % of Bacteria), but the specific identity of many could not be determined by FISH. Further investigation into the identity of other GAOs is necessary.

show abstract

“…It is known that EBPR capacity may deteriorate from the presence and often dominance of glycogen-accumulating organisms (GAO) under some operational conditions like low phosphorus/carbon (P/C) feeding ratio [4,5], low pH [6] and high temperature [7]. These GAO have metabolic functions similar to PAO except for accumulating glycogen instead of polyphosphate aerobically, while being morphologically and phylogenetically quite different to the known PAO.…”

Section: Introductionmentioning

confidence: 99%

Changes in respiratory quinone profiles of enhanced biological phosphorus removal activated sludge under different influent phosphorus/carbon ratio conditions

Ahn

Lee

Kwon

2006

Bioprocess Biosyst Eng

View full text Add to dashboard Cite

Changes in the microbial community of an enhanced biological phosphorus removal (EBPR) activated sludge system under different influent phosphorus/carbon (P/C) ratio conditions were investigated through evaluation of population respiratory quinone profiles. A total of 13 types of respiratory quinone homologs consisting of 3 types of ubiquinones (UQ) and 10 types of menaquinones (MK) were identified in this study. The dominant quinones were UQ-8 and MK-7 throughout the operational period. A higher P/C ratio (0.1) in the influent stimulated an increase in the mole fractions of UQ-8, MK-7, MK-8(H(4)), MK-9(H(4)) and MK-8(H(8)), suggesting that actinobacterial polyphosphate-accumulating organisms (PAO) containing partially hydrogenated MK, mainly MK-8(H(4)), were contributing to EBPR. However, when the P/C ratio gradually decreased from 0.1 to 0.01, the mole fractions of UQ-8 increased from 0.46 to 0.58, while MK-7, MK-8(H(2)), MK-8(H(4)), MK-9(H(4)), MK-8(H(8)) and MK-9(H(6)) markedly decreased. These changes in the respiratory quinone profiles suggest that glycogen-accumulating organisms corresponding to some Gammaproteobacteria had become dominant populations with a decrease in actinobacterial PAO. On the other hand, increasing abruptly the P/C ratio to 0.1 further caused an increase in the mole fraction of UQ-8, indicating that Rhodocyclus-related organisms were important PAO.

show abstract

Competition between polyphosphate- and glycogen-accumulating organisms in biological phosphorus removal systems – effect of temperature

Cited by 53 publications

References 5 publications

Are some putative glycogen accumulating organisms (GAO) in anaerobic : aerobic activated sludge systems members of the α-Proteobacteria?

Are some putative glycogen accumulating organisms (GAO) in anaerobic : aerobic activated sludge systems members of the α-Proteobacteria?

Anaerobic and aerobic metabolism of glycogen-accumulating organisms selected with propionate as the sole carbon source

Changes in respiratory quinone profiles of enhanced biological phosphorus removal activated sludge under different influent phosphorus/carbon ratio conditions

Contact Info

Product

Resources

About