Fluorescence in situ hybridization (FISH) was used to analyse the community composition of a sequencing batch reactor (SBR) operating with aerobic-anaerobic cycling and fed acetate as its sole carbon source. Phosphorus was removed from the SBR microbiologically. Marked shifts in the community structure occurred as the phosphorus/carbon (P/C) ratio in the feed was changed. When the P/C ratio was shifted from 1 :10 to 1 :50, FISH analysis showed that the percentage of β-Proteobacteria fell from ca 77 % of the total bacteria to ca 38 %. This decrease in the β-Proteobacteria coincided with a reduction in both the proportions of the β-proteobacterial Rhodocyclus-related phosphorus-accumulating bacteria and the biomass phosphorus content. Both the α-and β-proteobacterial ' G-bacterial ' populations assimilated acetate and synthesized PHA anaerobically. The α-Proteobacteria are considered responsible for glycogen production in these SBR systems.
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