Competition of cyclooctenes and cyclooctadienes for ethylene binding and activity in plants

Sisler, Edward C.; Blankenship, Sylvia M.; Guest, Margaret

doi:10.1007/bf00027443

Cited by 74 publications

(48 citation statements)

References 15 publications

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“…The release of bound ethylene from transgenic yeast showed a half-life of 12 h, a rate similar to that observed with one class of binding activity reported from severa1 plant sources (Sanders et al, 1991;Sisler, 1991). The binding of [14C]ethylene in yeast was also inhibited by transcyclooctene and norbornadiene, both competitive inhibitors of ethylene binding and action in plants (Sisler et al, 1990;Sisler, 1991). Thus, the characteristics of ethylene binding to the ETRl protein expressed in yeast are quite similar to in vivo binding sites previously observed in plant tissues (Sanders et al, 1991;Sisler, 1991).…”

Section: Interaction Of Ethylene Wlth the Etrl Proteinsupporting

confidence: 82%

The Mechanism of Ethylene Perception

Bleecker

Schäller²

1996

Plant Physiology

138

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Section: Interaction Of Ethylene Wlth the Etrl Proteinsupporting

confidence: 82%

The Mechanism of Ethylene Perception

Bleecker

Schäller²

1996

Plant Physiology

138

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“…Ethylene-binding assays carried out near the K d for ethylene binding to ETR1 The inhibitory effects of a compound on ethylene binding and action can be quantified by the determination of an apparent K I (Sisler et al, 1990;Sisler, 1991;Abeles et al, 1992). We determined an apparent K I for 1-MCP for its effects on the ethylene-growth response (K I ϭ 1.05 nL L Ϫ1 , gas) and for its effects on ethylene binding to ETR1 in transgenic yeast (K I ϭ 10.7 nL L Ϫ1 , gas).…”

Section: Effect Of 1-methylcyclopropene (1-mcp) On Ethylene Perceptionmentioning

confidence: 99%

Ethylene Perception by the ERS1 Protein in Arabidopsis

et al. 2000

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Ethylene perception in Arabidopsis is controlled by a family of five genes, including ETR1, ERS1 (ethylene response sensor 1), ERS2, ETR2, and EIN4. ERS1, the most highly conserved gene with ETR1, encodes a protein with 67% identity to ETR1. To clarify the role of ERS1 in ethylene sensing, we biochemically characterized the ERS1 protein by heterologous expression in yeast. ERS1, like ETR1, forms a membrane-associated, disulfide-linked dimer. In addition, yeast expressing the ERS1 protein contains ethylene-binding sites, indicating ERS1 is also an ethylene-binding protein. This finding supports previous genetic evidence that isoforms of ETR1 also function in plants as ethylene receptors. Further, we used the ethylene antagonist 1-methylcyclopropene (1-MCP) to characterize the ethylene-binding sites of ERS1 and ETR1. We found 1-MCP to be both a potent inhibitor of the ethylene-induced seedling triple response, as well as ethylene binding by yeast expressing ETR1 and ERS1. Yeast expressing ETR1 and ERS1 showed nearly identical sensitivity to 1-MCP, suggesting that the ethylene-binding sites of ETR1 and ERS1 have similar affinities for ethylene.

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“…Several compounds have shown to block the ethylene's linking site, thus inhibiting its effects (Sisler et al, 1990;Sisler, 1991). Both 2,5-norbornadiene and diazocyclopentadiene, as ethylene linkage inhibitors, can delay the softening and ripening in apples Sisler, 1989 andGong & Tian, 1998).…”

Section: Introductionmentioning

confidence: 99%

Shelf life of peaches treated with 1-methylcyclopropene

Kluge

Jacomino

2002

Sci. agric. (Piracicaba, Braz.)

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Peaches [Prunus persica (L.) Bastch] present reduced post-harvest shelf life, partially, due to their high respiratory rate and fast ripening. These processes are related to ethylene production, as well as its action. Peaches, cv. Aurora-1, were picked at two different ripening stages (mature green and ripe) and treated with 1-methylcyclopropene (1-MCP), the ethylene's competitive antagonist, at concentrations of 0, 100, 300 and 900 nL L -1 for 12 hours at 25 o C. After treatment, the fruits were stored at room temperature (25 o C) for six days. Fruits treated with 1-MCP presented better firmness and less ground color loss than nontreated fruits. The 1-MCP antagonist reduced the development of fruit rot at the mature green stage, but not at the ripe one. The 1-MCP shows commercial application potential in the treatment of peaches in order to delay the ripening process and increase their shelf life. Key words: Prunus persica, 1-MCP, ethylene, storage, firmness VIDA ÚTIL DE PÊSSEGOS TRATADOS COM 1-METILCICLOPROPENORESUMO: Pêssegos [Prunus persica (L.) Bastch] apresentam reduzida vida útil pós-colheita devido, em parte, a sua alta taxa respiratória e amadurecimento rápido. Estes processos estão relacionados com a produção e a ação do etileno. Pêssegos cv. Aurora-1 foram colhidos em dois estádios de maturação (verde e maduro) e tratados com o antagonista competitivo do etileno 1-metilciclopropeno (1-MCP) nas concentrações de 0, 100, 300 e 900 nL L -1 , durante 12 horas a 25 o C. Após o tratamentos os frutos foram armazenados em condições ambientais (25 o C) durante seis dias. Os frutos tratados com 1-MCP apresentaram maior firmeza de polpa e menor perda de coloração de fundo se comparado com os frutos não tratados. O 1-MCP reduziu o desenvolvimento de podridões em frutos do estádio verde, mas não em frutos maduros. O 1-MCP apresenta potencial de aplicação comercial em pêssegos, visando retardar o amadurecimento e aumentar sua vida útil de prateleira.

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Competition of cyclooctenes and cyclooctadienes for ethylene binding and activity in plants

Cited by 74 publications

References 15 publications

The Mechanism of Ethylene Perception

The Mechanism of Ethylene Perception

Ethylene Perception by the ERS1 Protein in Arabidopsis

Shelf life of peaches treated with 1-methylcyclopropene

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