Complement 1q-binding protein from Nile tilapia (Oreochromis niloticus): Molecular characterization, expression pattern upon bacterial infection and its binding properties

Chen, Meng; Liu, Shuo; Yan, Fangfang; Zhou, Enxu; Zhong, Xiaofang; Ding, Minghui; Ye, Jianmin

doi:10.1016/j.aquaculture.2018.09.060

Cited by 10 publications

(2 citation statements)

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“…Relative expressions were detected by qRT‐PCR with the help of 7500 Real Time PCR System (Applied Biosystems, USA). The β ‐actin (GenBank: EU871643.1) was used as the internal control (Chen et al., 2019; Fu et al., 2018; Zhou et al., 2020).…”

Section: Methodsmentioning

confidence: 99%

Functional characterization of TNF‐α in pufferfish (Takifugu obscurus) in immune response and apoptosis against Aeromonas hydrophila

Kong

Qian

et al. 2021

Journal of Fish Diseases

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Tumour necrosis factor‐α (TNF‐α) is a multifunctional cytokine involved in immune system homeostasis, antimicrobial defence, regulation of apoptosis, cell proliferation and differentiation. Although the pro‐inflammatory property of TNF‐α has been made new progress, detailed research on host defence against bacterial infection and inducing apoptosis remains to be revealed in early vertebrates. Here, we reported the TNF‐α homologue (ToTNF‐α) from pufferfish (Takifugu obscurus). The open reading frame (ORF) of ToTNF‐α was 753 bp, encoding a protein of 250 aa contained the TNF family signature and conserved cysteine residues. The mRNA expression of ToTNF‐α had a wide range of tested tissues, with the highest expression in the skin. After Aeromonas hydrophila infection, the mRNA expression of ToTNF‐α was significantly up‐regulated both in vivo and in vitro experiments. After stimulation by recombinant protein of ToTNF‐α ((r)ToTNF‐α), the relative expressions of endogenous TNF‐α, caspase 8, caspase 3, p53, and Bax inhibitor‐1 in head kidney leucocytes were all notably up‐regulated. These results showed that ToTNF‐α might induce apoptosis depend on pro‐ and anti‐apoptotic proteins at mRNA level. Moreover, flow cytometry analysis indicated that the (r)ToTNF‐α can induce apoptosis of head kidney leucocytes. Taken together, these characteristics suggest that ToTNF‐α can participate in immune response against A. hydrophila and induce apoptosis at mRNA and cellular level, which will help to understand the mechanism of apoptosis and immune response in teleost fish.

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Section: Methodsmentioning

confidence: 99%

Functional characterization of TNF‐α in pufferfish (Takifugu obscurus) in immune response and apoptosis against Aeromonas hydrophila

Kong

Qian

et al. 2021

Journal of Fish Diseases

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“…It was shown in 1993 that O. niloticus grew 60% faster than the other tilapias (Canonico et al, 2005). Besides, they have important characteristics to be selected as valuable aquaculture products, including the relationship between extreme environment conditions and sex-differences (Hekimoğlu et al, 2019;Dussenne et al, 2020), as a biomarker for disease control (Huang et al, 2018;Chen et al, 2019;Yin et al, 2018;Wu et al, 2019), and for evaluating the effects of environmental pollutants (Yıldırım et al, 2006;Benli & Ozkul, 2010;Beryl et al, 2019;Ibrahim, 2020). Hence, the Genetic Improvement of Farmed Tilapia (GIFT) program was established and GIFT has invested many types of research and projects to improve the farming performance of tilapias, especially in Asia (Dey et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

Notes on the Distribution of the Genus Oreochromis in the East Mediterranean Region of Turkey

Arslan¹,

İnnal²,

Özeren³

2021

Commagene Journal of Biology

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Tilapias are freshwater species which are the common name of three genera of cichlids, Oreochromis, Sarotherodon, and Coptodon. Many of the species belonged to these genera have been used for aquaculture products. Although their main native distribution areas are tropical and subtropical regions, they have spread to other areas due to their high invasive characteristics. This study was aimed to investigate the distribution of genus Oreochromis in the East Mediterranean Region of Turkey. The study was conducted at 18 stations located in Adana and Mersin from 2014 to 2017. The introduction of the species in these systems was mainly recorded weed control, transfers of fisherman, and escape from aquaculture facilities. However, the distribution of genus Oreochromis in Turkey and their effects on the aquatic ecosystems are unknown. The results showed that the species of genus Oreochromis were easily adapted the different conditions such as various flow rates, salinity, and temperature.

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Interleukin-10 (IL-10) participates in host defense against bacterial pathogens and promotes IgM antibody production in Nile tilapia (Oreochromis niloticus)

Duan

Kong

et al. 2021

Aquaculture

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Complement 1q-binding protein from Nile tilapia (Oreochromis niloticus): Molecular characterization, expression pattern upon bacterial infection and its binding properties

Cited by 10 publications

References 50 publications

Functional characterization of TNF‐α in pufferfish (Takifugu obscurus) in immune response and apoptosis against Aeromonas hydrophila

Functional characterization of TNF‐α in pufferfish (Takifugu obscurus) in immune response and apoptosis against Aeromonas hydrophila

Notes on the Distribution of the Genus Oreochromis in the East Mediterranean Region of Turkey

Interleukin-10 (IL-10) participates in host defense against bacterial pathogens and promotes IgM antibody production in Nile tilapia (Oreochromis niloticus)

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