Complement changes during leukapheresis

Rg, Strauss; Re, Spitzer; Stitzel, AE; Urmson, JR; Maguire, Lawrence C.; Koepke, J.A.; Thompson, Joanne

doi:10.1046/j.1537-2995.1980.20180125038.x

Cited by 26 publications

(10 citation statements)

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“…This saturation and/or deactivation may then result in diminished chemiluminescence upon stimulation. In contrast to filtration cytapheresis, complement activation is unlikely to occur during centrifugation cytapheresis (11). Complement acitvation is characteristically accompanied by increased chemiluminescence activity by stimulated cells (5,10) and clinical symptoms of pulmonary sequestration of leukocytes (9).…”

Section: Discussionmentioning

confidence: 99%

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On the Biocompatibility of IBM 2997 Continuous Flow Plateletapheresis

Lindena¹,

Burkhardt²,

Aulmann³

1990

Clinical Chemistry and Laboratory Medicine

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Summary:During the procedure of centrifugation cytapheresis donors occasionally experience adverse clincial reactions. We evaluated the possibility of whether activation of granulocytes and their subsequent release reactions, which may have been triggered by this extracorporeal circuit, were responsible for these adverse effects. Six blood samples were obtained during various set intervals during plateletapheresis. Of these, four samples were taken directly from each donor. The remaining two were drawn from the efferent lines, i.e. those which return blood from the cytapheresis machine back to the donor. Reactive oxygen species produced by granulocytes were monitored by chemiluminescence using microamounts of whole blood or isolated granulocytes. Furthermore, secreted granulocyte products such as neutral proteinase elastase, present in plasma in a complex with oti-proteinase inhibitor (complexed elastase), and lysosomal -glucuronidase were examined. A complete blood cell count, as well as values of haemoglobin, haematocrit, lactate dehydrogenase, protein, albumin and proteinase inhibitors such as oc 2 -macroglobulin and αϊ-proteinase inhibitor were also determined. Complexed elastase increased from a preapheresis value of about 140 μg/l to about 180 μg/l at the end of the cytapheresis. All other clinical chemical and cytological values were 8 to 12 percent lower than preapheresis values, which can be attributed to inherent plasma volume expansion. Reduced chemiluminescence was observed upon stimulation of phagocytes in the whole blood assay (about 700 counts/min χ ΙΟ 3 χ 50000 cells vs. about 600 counts/min χ ΙΟ 3 χ 50000 cells). This decrease was also seen with stimulated granulocytes (about 5800 counts/min χ ΙΟ 3 χ 50000 cells vs. about 4500 counts/min χ ΙΟ 3 χ 50000 cells). Unstimulated granulocytes, on the other hand, showed an increased native chemiluminescence. These data do not, however, indicate a cytapheresis mediated activation of the oxidative metabolism of granulocytes. Concomitant discharge of proteolytic enzymes remains, therefore, of no clinical importance.

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Section: Discussionmentioning

confidence: 99%

“…Investigations of complement activation (11) and functional analysis of monocytes and lymphocytes during cytapheresis have been reported (12,13). Similar studies with granulocytes, however, remain to be performed.…”

mentioning

confidence: 92%

On the Biocompatibility of IBM 2997 Continuous Flow Plateletapheresis

Lindena¹,

Burkhardt²,

Aulmann³

1990

Clinical Chemistry and Laboratory Medicine

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“…These results based on humoral parameters do not indicate a filtration-mediated impairment of the integrity of granulocytes, whereas erythrocytes were slightly fragile after 5 days of storage. It has been shown that plasma, after travelling through various extracorporeal circuits, äs commonly occurs in techniques such äs filtration cytapheresis (26), haemodialysis (7) and cardiopulmonary bypass (12), generates distinct granulocyte-activating products. These complement-splitting products (C5a) and immune complexes are potent Stimuli, causing increases in aggregation and respiratory burst activity (chemiluminescence) of granulocyte target cells.…”

Section: Discussionmentioning

confidence: 99%

Efficacy and Safety of a Polyester Leukocyte Removal Filter for Whole Blood and Red Cell Concentrate Filtration

Lindena

Papastavrou²,

Seidel³

1989

Clinical Chemistry and Laboratory Medicine

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Summary:Patients receiving multiple whole blood transfusions often experience adverse clinical Symptoms caused by leukocytes. In the present study, the efficacy and safety of a polyester filter for leukocytes (Sepacell R-500) was evaluated. Donor units of whole blood and red cell concentrate stored for varying lengths of time were filtered. Emphasis was placed on humoral parameters indicative of release and/or activation reactions of granulocytes (neutral proteinase elastase, lysozyme, aggregation, chemiluminescence) and erythrocytes l (lactate dehydrogenase, plasma haemoglobin). Nonspecific adsorption effects were investigated by plasma , .protein determinations (albumin, a 2 -macroglobulin). A complete blood cell count äs well äs values of 1 haemoglobin and haematocrit were determined. Sepacell R-500 proved to be a highly efficient filter to the j leukocyte depletion of blood. Our results of erythrocyte and granulocyte related humoral parameters provided no significant evidence of filtration mediated activation or releasing reactions of clinical consequence.

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“…Other studies by Nusbacheret al [43] and Strauss et al [44] showed that in addition to complement activation, absorption to the fil ter of some complement proteins, mainly properdin, occurs during LP and plays a role in reducing serum hemolytic titers.…”

Section: Leukapheresismentioning

confidence: 99%