2022
DOI: 10.1080/21505594.2022.2027629
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Complement evasion factor (CEF), a novel immune evasion factor of Streptococcus pyogenes

Abstract: Streptococcus pyogenes , a leading human pathogen, is responsible for a wide range of diseases, including skin and soft tissue infections and severe invasive diseases. S. pyogenes produces a large arsenal of virulence factors, including several immune evasion factors. We have identified an open reading frame ( spy0136 ) in the S. pyogenes SF370 genome encoding a protein of unknown function. Using recombinant Spy0136 in a pull-down … Show more

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Cited by 7 publications
(4 citation statements)
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“…To test if the two deacetylases contribute to the overall virulence of S. pyogenes , we used a human whole blood killing assay [ 30 ]. Freshly collected human blood was inoculated with 1 × 10 5 CFU S. pyogenes wildtype and mutant strains, and survival was analysed by enumerating the surviving bacteria ( Figure 6 A).…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…To test if the two deacetylases contribute to the overall virulence of S. pyogenes , we used a human whole blood killing assay [ 30 ]. Freshly collected human blood was inoculated with 1 × 10 5 CFU S. pyogenes wildtype and mutant strains, and survival was analysed by enumerating the surviving bacteria ( Figure 6 A).…”
Section: Resultsmentioning
confidence: 99%
“…This assay was carried out as described recently [ 30 ]. S. pyogenes wildtype and mutant strains were grown in BHI with appropriate antibiotics to the late exponential phase.…”
Section: Methodsmentioning
confidence: 99%
“…To combat the effects of the innate immune system, S. pyogenes expresses several enzymes degrading both complement components as well as chemokines and cytokines ( Table 1 ). In addition to expressing complement-degrading enzymes, S. pyogenes expresses other virulence factors inhibiting complement functions, such as the recently described complement evasion factor (CEF) and streptococcal inhibitor of complement (SIC) [ 37 , 38 ], both outside the scope of this review. Furthermore, proteolysis and modification of extracellular matrix (ECM) components that can alter barrier functions will not be discussed here (for a summary of SpeB’s activities on ECM, see [ 12 ]).…”
Section: Main Functional Categories Of S Pyogenes ...mentioning
confidence: 99%
“…Genetic engineering approaches are necessary to better understand the molecular basis of important aspects of bacterial physiology, such as virulence and metabolism. The technology used for genetic engineering enables researchers to identify genetic functions by deleting any gene of interest 1 in bacteria. This technique has been developed and improved for years, contributing to the scientific field based mainly on model bacterial organisms, such as Escherichia coli and Bacillus subtilis .…”
Section: Introductionmentioning
confidence: 99%