Complement-Fixation in Infections with the Virus of Lymphocytic Choriomeningitis

Smadel, Joseph E.; Baird, Robert D.; Wall, Margaret J.

doi:10.3181/00379727-40-10309p

Cited by 28 publications

(8 citation statements)

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“…These experiments demonstrate that the baboon is susceptible to LCM virus as are humans and other primates such as rhesus, cynomolgus, and eebus monkeys (3,4,5,7,8,12,15,17,19,21). All three baboons, when inoculated s.c. with a moderate to high dose of LCM virus (strain WE3), developed slight malaise and fever lasting for a few days.…”

Section: Discussionmentioning

confidence: 66%

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Fetal infection of the baboon(Papio cynocephalus) with lymphocytic choriomeningitis virus

Ackermann

Kalter

Heberling

et al. 1979

Archives of Virology

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Recent observations of LCM-induced fetal damage in humans suggested attempts to develop an animal model for studies on viral congenital malformations. We report herein viral studies on three pregnant baboons (Papio cynocephalus) inoculated subcutaneously with LCM virus strain WE3. The first animal, inoculated in the 9th week of pregnancy, aborted 9 days after a high virus dose. Inoculation of the second baboon during a later stage (23rd week) of pregnancy with a moderate virus dose, resulted in the demonstration of virus in the placenta, amniotic fluid, and cord blood. The infant showed only a slight pleocytosis of the spinal fluid, but no virus shedding and no late sequelae. The third baboon inoculated with a high virus dose during the 21st week of pregnancy delivered an underweight, icteric infant that succumbed on the 6th day. All organs of this animal that were tested contained virus. Microscopic examination of these tissues revealed multifocal necrosis, cerebral glial nodules, meningitis, and bilateral choriovasculitis. These results illustrate that fetal damage observed in the LCM-inoculate baboon resembles that seen in humans following infection with LCM virus.

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Section: Discussionmentioning

confidence: 66%

“…LCM virus antigen was prepared from guinea pig lungs as described by S~ADEL et al (21). Guinea pig LCM hyperimmune serum was used as a control.…”

Section: Complement Fixation Testmentioning

confidence: 99%

Fetal infection of the baboon(Papio cynocephalus) with lymphocytic choriomeningitis virus

Ackermann

Kalter

Heberling

et al. 1979

Archives of Virology

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“…Since there is a noninfectious soluble antigen associated with this virus (8), the CF test does 306 E. Traub: not accurately measure the infectivity of a tissue extract. Experience has shown, however, that a rough para]lelism exists between the infectivity and CF titers of various organs in tolerant mice.…”

Section: Strains O/ Virus"mentioning

confidence: 99%

Observations on immunological tolerance and ?Immunity? in mice infected congenitally with the virus of lymphocytic choriomeningitis (LCM)

Traub¹

1960

Archiv f Virusforschung

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Mice infected in utero with LCM virus carry large amounts of active virus in their blood and organs for many months and discharge considerable quantities of the agent with their secretions and excretions (11). This observation indicates continuous multiplication of the virus in the organs of such animals, which look like normal individuals in spite of their chronic infection. Intrauterine infection leads to a stable equilibrium between virus and body. It was also observed that mice infected in utero would develop practically no specific antibodies, while the sera of mature mice infected experimentally or by contact at least gave positive complement-fixation (CF) tests (9,14).

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“…L6pine and his coworkers, like Howitt, used a crude extract of infected tissue as antigen. About this time experiments reported by us (1) showed that anti-soluble substance antibodies develop after infection in man and guinea pigs and are responsible for complement fixation. Subsequently, work elsewhere and in our laboratory confirmed Howitt's original report that serum from hyperimmune mice fixes complement with preparations from infected tissue; crude tissue extracts (18,22) and soluble antigen (23) were employed.…”

Section: Antibodies Of Lymphocytic Ciioriomieningitismentioning

confidence: 99%

“…(Received for publication, June 28, 1940) Lymphocytic choriomeningitis has been shown to belong to the group of virus diseases which is characterized by the occurrence in infected tissues of a soluble antigen separable from the virus (1,2). The presence of the specific soluble antigen of lymphocytic choriomeningitis was demonstrated in extracts of various organs of acutely ill guinea pigs, mice, and monkeys by means of complement fixation (2).…”

Section: (From the Hospital Of The Rockefeller Institute For Medical mentioning

confidence: 99%

A Soluble Antigen of Lymphocytic Choriomeningitis

Smadel

Wall

1940

Journal of Experimental Medicine

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Anti-soluble substance antibodies and neutralizing substances, which develop following infection with the virus of lymphocytic choriomeningitis, appear to be separate entities. The times of appearance and regression of the two antibodies are different in both man and the guinea pig; the antisoluble substance antibodies appear earlier and remain a shorter time. Moreover, mice develop them but no demonstrable neutralizing substances. Injection of formalin-treated, virus-free extracts containing considerable amounts of soluble antigen fails to elicit anti-soluble substance antibodies and to induce immunity in normal guinea pigs; administration of such preparations to immune pigs, however, is followed by a marked increase in the titer of anti-soluble substance antibodies in their serum. On the other hand, suspensions of formolized washed virus are effective in normal guinea pigs in stimulating both anti-soluble substance antibodies and protective substances, and in inducing immunity to infection.

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Complement-Fixation in Infections with the Virus of Lymphocytic Choriomeningitis

Cited by 28 publications

References 0 publications

Fetal infection of the baboon(Papio cynocephalus) with lymphocytic choriomeningitis virus

Fetal infection of the baboon(Papio cynocephalus) with lymphocytic choriomeningitis virus

Observations on immunological tolerance and ?Immunity? in mice infected congenitally with the virus of lymphocytic choriomeningitis (LCM)

A Soluble Antigen of Lymphocytic Choriomeningitis

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