Complement Fixation Reaction and Agar Gel Double Diffusion Test in Tyzzer's Disease of Mice

Fujiwara, Kôsaku

doi:10.1111/j.1348-0421.1967.tb00326.x

Cited by 21 publications

(9 citation statements)

References 4 publications

(3 reference statements)

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“…As reported previously [1,4], the infectivity of Tyzzer's disease agents decreases rapidly in vitro showing rapid bacteriolysis even at low temperature. Moreover, the number of organisms detected from the liver at the initial stage of the infection was shown to be much reduced as compared to that which had been inoculated.…”

Section: Discussionsupporting

confidence: 72%

Bacteremia in Experimental Tyzzer's Disease of Mice

Takagaki

Fujiwara

1968

Japanese Journal of Microbiology

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Bacteremia was observed during the late stage of experimental Tyzzer's disease in mice. The number of organisms in the blood in mice treated with cortisone increased markedly in the infection with highly virulent organisms, whereas bacteremia was of low incidence and less severe in infections with low virulence organisms. The number of organisms in the blood stream was shown to increase linearly during the course of fatal infection attaining a maximum level of 107 organisms per ml blood. The number of organisms in the blood was found to be closely related to the number in the liver when bacteremia was observed. In the peripheral blood, organisms were first detectable when the number of organisms in the liver gained a level of 107 per g tissue, and the subsequent increase in the number of bacteria in the blood was approximately 3 times more rapid than in the liver. The organisms in the blood were comparable to those in the liver morphologically as well as in pathogenicity. Histopathological examination frequently revealed liberation of organisms from liver cells into sinusoids. There was no evidence of significant multiplication of the organisms in organs other than the liver.

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Section: Discussionsupporting

confidence: 72%

Bacteremia in Experimental Tyzzer's Disease of Mice

Takagaki

Fujiwara

1968

Japanese Journal of Microbiology

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“…It would be especially desirable to develop serodiagnostic methods of detection, as diagnosis based on provocation and identification of the bacterium is laborious and sometimes unspecific. Fujiwara (1967) demonstrated antibodies in mouse immune serum against a B. pillformis antigen by the complement fixation (CF) test and by immunodiffusion. He subsequently found the CF test useful for testing sera from retired breeder mice, but not for testing sera from weanlings (Fujiwara, 1971).…”

mentioning

confidence: 99%

Studies on Tyzzer's disease : application of immunofluorescence for detection of Bacillus piliformis and for demonstration and determination of antibodies to it in sera from mice and rabbits

Fries¹

1977

Lab Anim

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SummaryBacillus pilifarmis antigens were demonstrated in smear preparations from infected mouse livers by direct immunofluorescence technique. Mouse serum antibodies against B. pilifarmis were demonstrated by indirect immunofluorescence technique. The test was employed quantitatively both on sera from experimentally infected mice and on sera from clinically healthy mice from colonies infected with B.

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“…The material used for electron microscope preparations was highly effective when used as a vaccine to protect mice from challenge infection [4], and soluble antigen prepared from the infected liver without addition of formalin reacted in both complement fixation and double diffusion tests using immune mouse serum [2]. Next, immune fluorescence technique to demonstrate possible flagella was utilized.…”

Section: Notesmentioning

confidence: 99%