Complement in human reproduction: Activation and control

Rooney, Isabelle; Oglesby, Teresa J.; Atkinson, John P.

doi:10.1007/bf02918258

Cited by 87 publications

(40 citation statements)

References 106 publications

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“…By Western blotting using mAb anti-CD59 (Fig. 8 B), a 20-kD band was detected in fractions of the first peak, whereas no anti-CD59 material was detected in fractions [26][27][28][29][30].…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

GPI-anchored complement regulatory proteins in seminal plasma. An analysis of their physical condition and the mechanisms of their binding to exogenous cells.

Rooney¹,

Heuser²,

Atkinson³

1996

J. Clin. Invest.

143

107

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We analyzed and compared the properties of three glycosylphosphatidylinositol (GPI)-anchored proteins, CD59, CD55 (both C regulators), and CDw52, and of the transmembrane C regulator CD46 in seminal plasma (SP). We demonstrated previously that anchor-intact SP CD59 is present on the membranes of vesicles (prostasomes) and that cells acquire this protein during incubation with SP. We now report that this acquisition is due partly to adherence of prostasomes to cells and partly to a second mechanism which may involve micellar intermediates. Using fluorescent labeling, ultracentrifugation, and density gradient centrifugation, virtually all CD46 was present on prostasomes whereas CD59, CD55, and CDw52 were also detected in a form which remained in the 200,000 g supernatant and equilibrated at higher density than prostasomes in gradients. All three GPI-linked proteins eluted at high molecular mass during size exclusion chromatography of this nonprostasome

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“…By Western blotting using mAb anti-CD59 (Fig. 8 B), a 20-kD band was detected in fractions of the first peak, whereas no anti-CD59 material was detected in fractions [26][27][28][29][30].…”

Section: Resultsmentioning

confidence: 99%

“…Sperm are at risk of C attack within the female reproductive tract (28) and C inhibitors may be of importance for fertility. Further, sperm acquire new proteins during passage through the epididymis (29).…”

Section: Discussionmentioning

confidence: 99%

GPI-anchored complement regulatory proteins in seminal plasma. An analysis of their physical condition and the mechanisms of their binding to exogenous cells.

Rooney¹,

Heuser²,

Atkinson³

1996

J. Clin. Invest.

143

107

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“…Cells of the reproductive system have also been shown to express various complement regulatory proteins and receptors. This includes spermatozoa, oocytes, and, as expected, fibroblasts, and epithelial and endothelial cells in the placenta (Anderson et al, 1993;Rooney et al, 1993). Granulocytes-including neutrophils, monocytes, basophils, and mast cells-as well as hepatocytes, smooth muscle cells, and endothelial and epithelial cells express the receptor for C5a (Gerard and Gerard, 1994;Murphy, 1994;Haviland et al, 1995;Wetsel, 1995).…”

Section: Tissue Sites Of Complement Synthesismentioning

confidence: 88%

“…The role of complement in host defense is highly diverse, ranging from mediating inflammatory responses through increasing vascular permeability, chemotaxis, and opsonizing pathogens for phagocytosis, to activation of B cells and serving in ligand-receptor interactions in reproduction (Volanakis and Fearon, 1992;Rooney et al, 1993;Simpson and Holmes, 1994). For the purpose of this review, only a brief summary of these activities will be covered, particularly as they relate to C3 and C5, since most of the host defense activities of complement are Figure 1.…”

Section: The Complement Systemmentioning

confidence: 99%

Complement Biosynthesis in the Central Nervous System

Barnum

1995

Critical Reviews in Oral Biology & Medicine

187

103

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ABSTRACT:Complement is an important effector arm of the human immune response. Binding of proteolytic fragments derived from activation of complement by specific receptors leads to responses as diverse as inflammation, opsonization, and B-cell activation. The importance of characterizing the expression and regulation of complement in the CNS is highlighted by growing evidence that complement plays a significant role in the pathogenesis of a variety of neurological diseases, such as multiple sclerosis and Alzheimer's disease. \n vitro studies have demonstrated that astrocytes, the predominant glial cell type in the brain, are capable of expressing or producing a majority of the components of the complement system. Expression of many complement proteins synthesized by astrocytes is regulated by both pro-and anti-inflammatory cytokines, many of which are also produced by several cell types in the CNS. In addition to astrocytes, ependymal cells, endothelial cells, microglia, and neurons have recently been shown to synthesize various complement proteins or express complement receptors on their cell surfaces. Together, these studies demonstrate that several cell types throughout the brain have the potential to express complement and, in many cases, increase expression in response to mediators of the acute phase response. These studies suggest that complement may play a greater role in CNS immune responses than previously thought, and pave the way for better understanding of the dynamics of complement expression and regulation in vivo. Such understanding may lead to therapeutic manipulation of complement host defense functions in a variety of inflammatory and degenerative diseases in the CNS.

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“…CD59 expression is limited to the plasma membrane, and DAF is expressed on both the plasma membrane and the IAM. (23). Second, the hypoglycosylated form of MCP expressed on human and New World monkey sperm (Fig.…”

Section: Mcp Is Expressed On the Iam In The Marmosetmentioning

confidence: 99%

Cutting Edge: Inhibiting Measles Virus Infection but Promoting Reproduction: An Explanation for Splicing and Tissue-Specific Expression of CD46

Riley

Tannenbaum

Abbott

et al. 2002

The Journal of Immunology

Self Cite

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Membrane cofactor protein (MCP; CD46) regulates the complement cascade by inhibiting C3b and C4b deposited on self tissue. This function resides in the complement control protein repeats (CCPs), with CCPs 2–4 essential for regulation. MCP is expressed on the inner acrosomal membrane of human sperm, and Abs to CCP1 inhibit sperm-egg interactions. In somatic tissues, New World monkeys express an alternatively spliced form of MCP lacking CCP1. Although retaining complement-regulatory activity, this form is postulated to render these species less susceptible to strains of the measles virus whose hemagglutinin requires CCP1 and CCP2 for attachment. Using PCR, sequencing, Western blotting, and immunohistochemistry, we characterized MCP expression in the testes and sperm of two New World monkeys. In these species, sperm express MCP bearing CCP1. The germ cell-specific expression pattern of this domain strongly suggests an evolutionarily conserved role for MCP in fertilization.

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Complement in human reproduction: Activation and control

Cited by 87 publications

References 106 publications

GPI-anchored complement regulatory proteins in seminal plasma. An analysis of their physical condition and the mechanisms of their binding to exogenous cells.

GPI-anchored complement regulatory proteins in seminal plasma. An analysis of their physical condition and the mechanisms of their binding to exogenous cells.

Complement Biosynthesis in the Central Nervous System

Cutting Edge: Inhibiting Measles Virus Infection but Promoting Reproduction: An Explanation for Splicing and Tissue-Specific Expression of CD46

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