Complement system in zebrafish

Zhang, Shicui; Cui, Pengfei

doi:10.1016/j.dci.2014.01.010

Cited by 90 publications

(54 citation statements)

References 85 publications

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“…In the present study, we also observed that the CPF exposure did not change the expression of C3 (data not shown) in larval zebrafish but C4 (Fig. 6H), which is also capable of promoting proinflammatory reactions by modulating innate immune responses [64,65].…”

Section: Discussionsupporting

confidence: 73%

The toxicity of chlorpyrifos on the early life stage of zebrafish: A survey on the endpoints at development, locomotor behavior, oxidative stress and immunotoxicity

Jin

Liu

Peng

et al. 2015

Fish & Shellfish Immunology

204

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Section: Discussionsupporting

confidence: 73%

The toxicity of chlorpyrifos on the early life stage of zebrafish: A survey on the endpoints at development, locomotor behavior, oxidative stress and immunotoxicity

Jin

Liu

Peng

et al. 2015

Fish & Shellfish Immunology

204

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“…This work is also a first step toward the immune function of ftr genes. The diversity of finTRIM, like the one of other fish genes involved in immunity such as ISG (43), complement factors (58), or heparan sulfate producing enzymes (59), provides many opportunities for sub-functionalization and will likely require systematic screening approaches.…”

Section: Discussionmentioning

confidence: 99%

FTR83, a Member of the Large Fish-Specific finTRIM Family, Triggers IFN Pathway and Counters Viral Infection

et al. 2017

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Tripartite motif (TRIM) proteins are involved in various cellular functions and constitute key factors of the antiviral innate immune response. TRIM proteins can bind viral particles directly, sending them to degradation by the proteasome, or ubiquitinate signaling molecules leading to upregulation of innate immunity. TRIM proteins are present in across metazoans but are particularly numerous in vertebrates where genes comprising a B30.2 domain have been often duplicated. In fish, a TRIM subset named finTRIM is highly diversified, with large gene numbers and clear signatures of positive selection in the B30.2 domain suggesting they may be involved in antiviral mechanisms. finTRIM provides a beautiful model to investigate the primordial implication of B30.2 TRIM subsets in the arsenal of vertebrate antiviral defenses. We show here that ftr83, a zebrafish fintrim gene mainly expressed in the gills, skin and pharynx, encodes a protein affording a potent antiviral activity. In vitro, overexpression of FTR83, but not of its close relative FTR82, induced IFN and IFN-stimulated gene expression and afforded protection against different enveloped and non-enveloped RNA viruses. The kinetics of IFN induction paralleled the development of the antiviral activity, which was abolished by a dominant negative IRF3 mutant. In the context of a viral infection, FTR83 potentiated the IFN response. Expression of chimeric proteins in which the B30.2 domain of FTR83 and the non-protective FTR82 had been exchanged, showed that IFN upregulation and antiviral activity requires both the Ring/BBox/Coiled coil domain (supporting E3 ubiquitin ligase) and the B30.2 domain of FTR83. Finally, loss of function experiments in zebrafish embryos confirms that ftr83 mediates antiviral activity in vivo. Our results show that a member of the largest TRIM subset observed in fish upregulates type I IFN response and afford protection against viral infections, supporting that TRIMs are key antiviral factors across vertebrates.

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“…The zebrafish (Danio rerio) has been an ideal organism for the study of developmental biology and biotechnology, but it is emerging as a model species for the study of immunology, human disease and cancer (Trede et al, 2004;van der Sar et al, 2004;Phelps and Neely, 2005;Meeker and Trede, 2008;Sullivan and Kim, 2008;Zhang and Cui, 2014). Eggs of zebrafish are released and fertilized externally, and the resulting embryos/larvae are therefore exposed to an aquatic environment full of potential pathogens capable of causing various types of diseases.…”

Section: Introductionmentioning

confidence: 99%

Functional characterization of chitinase-3 reveals involvement of chitinases in early embryo immunity in zebrafish

Teng

Sun

Liu

et al. 2014

Developmental & Comparative Immunology

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Complement system in zebrafish

Cited by 90 publications

References 85 publications

The toxicity of chlorpyrifos on the early life stage of zebrafish: A survey on the endpoints at development, locomotor behavior, oxidative stress and immunotoxicity

The toxicity of chlorpyrifos on the early life stage of zebrafish: A survey on the endpoints at development, locomotor behavior, oxidative stress and immunotoxicity

FTR83, a Member of the Large Fish-Specific finTRIM Family, Triggers IFN Pathway and Counters Viral Infection

Functional characterization of chitinase-3 reveals involvement of chitinases in early embryo immunity in zebrafish

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