Complete amino acid sequence of the regulatory light chain of obliquely striated muscle myosin from earthworm, Lumbricus terrestris

Abstract

Amino acid sequence analysis of the regulatory light chain of obliquely striated muscle myosin from earthworm, Lumbricus terrestris, was performed completely. The polypeptide consists of 195 amino acids with a calculated molecular mass of 21 943 Da. From the arrangement of amino acid residues, the first EF‐hand domain appears to be a specific Ca2+ ‐binding site. The unusually long N‐terminal region of about 40 amino acids, which is characterized by accumulation of basic amino acids, is similar to that of the myosin A1 catalytic light chain from rabbit skeletal muscle.

Invertebrate muscles: Thin and thick filament structure; molecular basis of contraction and its regulation, catch and asynchronous muscle

“…Despite the evolutionary closeness of the groups, Nemertean and Echiuran actomyosin has been identified as solely myosin regulated Lehman and Szent-Györgyi, 1975;Chantler and Szent-Györgyi, 1978) but annelid actomyosin as dually regulated Lehman and Szent-Györgyi, 1975;Royuela et al, 1996Royuela et al, , 2000a. The annelid dual regulation is well confirmed, with annelid myosin being regulated by direct Ca ++ binding (D'Haese, 1980;Carlhoff and D'Haese, 1987;Serwe et al, 1993;Ravaux et al, 2001) and skeletal muscle troponin regulating annelid tropomyosin in a Ca ++ dependent manner (Ditgens et al, 1982). Alternatively, given the sensitivity of thin and thick filament regulatory systems to extraction conditions, it is unclear if the single regulation observed in the Annelid sister groups is real.…”

Photoaffinity Labeling of Human Lysosomal β-Hexosaminidase B

“…Separation was achieved with HPLC grade water, containing 0.2% hexafluoroacetone (adjusted with 25% aqueous NH 3 to pH 7.0) as solvent A and 84% acetonitrile in water containing 0.03% hexafluoroacetone as solvent B (21). The reverse phase column was equilibrated with 4% solvent B at ambient temperature.…”

“…However, its calculated specific radioactivity (0.066 Ci/mmol) was rather low and suggested a contamination by an unlabeled peptide. Therefore, the peak was rechromatographed in the solvent system of Serwe et al (21) (0.2% hexafluoroacetone in water, pH 7.0, versus 0.03% hexafluoroacetone and 84% acetonitrile in water). As shown in Fig.…”

Proteomic and metabolomic analysis of earthworm Eisenia fetida exposed to different concentrations of 2,2′,4,4′-tetrabromodiphenyl ether

“…The down-regulation of PDI could imply that BDE 47 (10 μg/L) affected the antioxidant system by the induction of oxidative stress in E. fetida, which was indicated by elevated SOD activity. Myosin regulatory light chains are regulators in the myosin contractile activity that is related to muscle contraction [57]. Fructose-bisphosphate aldolase (FBPA) is a ubiquitous enzyme that is essential for glycolysis and gluconeogenesis [58].…”