Complete amino acid sequence of γ‐subunit of the GTP‐binding protein from cattle retina

Abstract

The complete amino acid sequence of the y-subunit of the GTP-binding protein from cattle retina has been established. The polypeptide chain of the y-subunit consists of 69 amino acid residues and contains the unusual sequence Cys35-Cys36. The h4,of the y-subunit is 8008.7. GTP-binding protein y-SubunitAmino acid sequence Rhodopsin

G Proteins: Transducers of Receptor-Generated Signals

“…cDNAs that encode Gt')' have been cloned and sequenced (105,106); the protein has been sequenced as well (107). Gt')' has 74 amino acid residues (Mr = 8400) and is very hydrophilic and acidic .…”

A structural model for the α‐subunit of transducin Implications of its role as a molecular switch in the visual signal transduction mechanism

“…Biochemical data on the structural and functional relationships of the T, molecule are now available and include the primary sequence elucidated via molecular cloning [4-71, peptide mapping [8], and functional domain analysis through chemical modification [9,10]. Moreover, the homology between the GTP-binding site of T, and those of the G-proteins coupled to adenylate cyclase, elongation factor Tu (EF-Tu) and the ras p21 protein has been identified [4][5][6][7]111. Recently, the tertiary structure of the GTP-binding domain of EF-Tu was solved to a resolution of 2.7 A by Xray crystallography [12,13] and has served as a model for the ras gene product [14] and for the partial folding of a consensus GTP-binding site [15].…”

Antisera of designed specificity for subunits of guanine nucleotide-binding regulatory proteins.

“…Although the relationship of the two proteins is not clear, P subunits from Gs, Gi, and T are indistinguishable by peptide mapping and amino acid analysis (10). The y subunit (-8 kDa) of the G proteins is presumed to be similar; the amino acid sequence of TY has been determined (11,12). Little is known about the function of this subunit.…”