Complete assignment of the 1H and 13C NMR spectra of four triterpenes of the ursane, artane, lupane and friedelane groups

Alves, Josinete S.; Castro, Janiza C. M. de; Freire, Maisa O.; da‐Cunha, Emidio V. Leitão; Barbosa‐Filho, José Maria; Silva, Marcelo Sobral de

doi:10.1002/(sici)1097-458x(200003)38:3<201::aid-mrc622>3.3.co;2-s

Cited by 13 publications

(14 citation statements)

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“…In both cases together with the p19 suppressor of silencing and the gene encoding 1-deoxy-d-xylulose 5-phosphate synthase CfDXS. NMR analysis of the isolated compounds and comparison to previously reported data [47,48] identified compound 5 as 29-hydroxy-friedelin and compound 6 as polpunonic acid (29-carboxy-friedelin; Fig. 1, Additional file 1: Table S3).…”

Section: Structural Elucidation Of the Cyp712k1 Cyp712k2 And Cyp712kmentioning

confidence: 82%

Integrating pathway elucidation with yeast engineering to produce polpunonic acid the precursor of the anti-obesity agent celastrol

et al. 2020

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Background: Celastrol is a promising anti-obesity agent that acts as a sensitizer of the protein hormone leptin. Despite its potent activity, a sustainable source of celastrol and celastrol derivatives for further pharmacological studies is lacking. Results:To elucidate the celastrol biosynthetic pathway and reconstruct it in Saccharomyces cerevisiae, we mined a root-transcriptome of Tripterygium wilfordii and identified four oxidosqualene cyclases and 49 cytochrome P450s as candidates to be involved in the early steps of celastrol biosynthesis. Using functional screening of the candidate genes in Nicotiana benthamiana, TwOSC4 was characterized as a novel oxidosqualene cyclase that produces friedelin, the presumed triterpenoid backbone of celastrol. In addition, three P450s (CYP712K1, CYP712K2, and CYP712K3) that act downstream of TwOSC4 were found to effectively oxidize friedelin and form the likely celastrol biosynthesis intermediates 29-hydroxy-friedelin and polpunonic acid. To facilitate production of friedelin, the yeast strain AM254 was constructed by deleting UBC7, which afforded a fivefold increase in friedelin titer. This platform was further expanded with CYP712K1 to produce polpunonic acid and a method for the facile extraction of products from the yeast culture medium, resulting in polpunonic acid titers of 1.4 mg/L. Conclusion:Our study elucidates the early steps of celastrol biosynthesis and paves the way for future biotechnological production of this pharmacologically promising compound in engineered yeast strains.

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Section: Structural Elucidation Of the Cyp712k1 Cyp712k2 And Cyp712kmentioning

confidence: 82%

Integrating pathway elucidation with yeast engineering to produce polpunonic acid the precursor of the anti-obesity agent celastrol

et al. 2020

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“…For example, several of the c. 250 species of the genus Erythroxylum investigated for chemical composition contained alkaloids and terpenes, especially triterpenes in the leaves and fruits (Zuanazzi et al, 2001;Barreiros et al, 2002). Triterpenes are also found in M. obtusifolia (Alves et al, 2000). Species of Araceae are known to contain saponins and calcium oxalate crystals that may cause kidney failure in vertebrates (Knight & Walter, 2003).…”

Section: Discussionmentioning

confidence: 99%

How much fruit do fruit‐eating frogs eat? An investigation on the diet of Xenohyla truncata (Lissamphibia: Anura: Hylidae)

Silva

Britto-Pereira

2006

Journal of Zoology

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This paper presents the results of a 22-month survey and the examination of the intestinal content of 356 specimens of Xenohyla truncata (Anura: Hylidae) from Restinga de Maric´a, Rio de Janeiro, Brazil. Our results confirm prior observations that fruits are intrinsic to the diet of these frogs. In addition, these new data increase our understanding of the relationship between frogs and the plants they feed upon. Plant consumption follows availability of fruits in the area, indicating that the diversity of fruits consumed by the frogs does not represent choice, but rather plant phenology and fruit availability.

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“…Our group has also reviewed the medicinal and poisonous plants of the Northeastern region of Brazil (Agra et al, 2007(Agra et al, , 2008, among other review articles (Alves et al, 2000;Souza et al, 2005;Gonçalves et al, 2006;BarbosaFilho et al, 2007bBarbosaFilho et al, , 2008Sena-Filho et al, 2008).…”

Section: Plants Of the American Continent With Antimalarial Activitymentioning

confidence: 99%

Plants of the American continent with antimalarial activity

Mariath¹,

Falcão²,

Barbosa-Filho³

et al. 2009

Rev. bras. farmacogn.

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RESUMO: "Plantas do continente Americano com atividade antimalárica". Malária é uma doença parasitária humana causada por protozoários do gênero Plasmodium. Esta doença tem acometido populações que habitam regiões tropicais e subtropicais. Anualmente, cerca de 500 milhões de casos ocorrem no mundo, o que permite ser considerada uma doença emergente de importância para a saúde pública. Neste contexto, os produtos naturais, a exemplo das espécies vegetais, têm suas moléculas bioativas como alvo para estudos farmacológicos, toxicológicos e fitoquímicos destinados à síntese de medicamentos mais eficazes para o tratamento de inúmeras doenças. Portanto, este trabalho fornece subsídio às pesquisas com produtos naturais para o tratamento da malária. Nesta revisão, 476 espécies de plantas do continente Americano foram relatadas para a atividade antimalárica, sendo destas 198 ativas e 278 inativas para algum tipo de Plasmodium, quando avaliados através de modelos in vitro e in vivo.Unitermos: Atividade antimalárica, plantas medicinais, produtos naturais, continente Americano, revisão.ABSTRACT: Malaria is a human parasitic disease caused by protozoa species of the Plasmodium genus. This disease has affected populations of the tropical and subtropical regions. About 500 million new cases occur annually on the world and therefore it is considered an emerging disease of important public health problem. In this context, the natural products as vegetables species have their bioactive molecules as targets for pharmacological, toxicological and phytochemical studies towards the development of more effective medicines for the treatment of many diseases. So this work intends to aid the researchers in the study of natural products to the treatment of malaria. In this review, 476 plants of the American continent were related for the antimalarial activity and of these vegetables species 198 were active and 278 inactive for some type of Plasmodium when they were evaluated through of in vitro or in vivo bioassays models.

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Complete assignment of the 1H and 13C NMR spectra of four triterpenes of the ursane, artane, lupane and friedelane groups

Cited by 13 publications

References 0 publications

Integrating pathway elucidation with yeast engineering to produce polpunonic acid the precursor of the anti-obesity agent celastrol

Integrating pathway elucidation with yeast engineering to produce polpunonic acid the precursor of the anti-obesity agent celastrol

How much fruit do fruit‐eating frogs eat? An investigation on the diet of Xenohyla truncata (Lissamphibia: Anura: Hylidae)

Plants of the American continent with antimalarial activity

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