1967
DOI: 10.1038/214609b0
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Complete Development of the Human Hookworm, Necator americanus, in Golden Hamsters, Mesocricetus auratus

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Cited by 55 publications
(31 citation statements)
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“…N. americanus was maintained in hamsters (32), and Ancylostoma caninum was maintained in experimental beagles (18). Adult worms were removed from the intestines of euthanized animals, washed in phosphate-buffered saline (PBS), and then cultured overnight in RPMI 1640 containing 100 U/ml penicillin G sodium, 100 g/ml streptomycin sulfate, and 0.25 g/ml amphotericin B (all reagents from SigmaAldrich, St. Louis, MO).…”
Section: Methodsmentioning
confidence: 99%
“…N. americanus was maintained in hamsters (32), and Ancylostoma caninum was maintained in experimental beagles (18). Adult worms were removed from the intestines of euthanized animals, washed in phosphate-buffered saline (PBS), and then cultured overnight in RPMI 1640 containing 100 U/ml penicillin G sodium, 100 g/ml streptomycin sulfate, and 0.25 g/ml amphotericin B (all reagents from SigmaAldrich, St. Louis, MO).…”
Section: Methodsmentioning
confidence: 99%
“…Despite being a human parasite, N. americanus can also be maintained in the golden hamster. 5 Therefore, to confirm the data obtained using individual skin macromolecules, the effects of proteinase inhibitors on the penetration of live larvae through excised hamster skin was also studied.…”
mentioning
confidence: 90%
“…5 Infective larvae were cultured from fecal material by a method modified from Harada and Mori 6 and previously described by Kumar and Pritchard. 3 Cultured larvae were stored in 50 mM Na 2 HPO4, 70 mM NaCl, 15 mM KH 2 PO 4 , pH 7.4, until used.…”
Section: Preparation Of N Americanus Larval Es Productsmentioning
confidence: 99%
“…Necator americanus was maintained in syngeneic DSN hamsters, as described by Sen and Seth (1967) and the third stage infective larvae cultured from faecal material by a method modified from Harada and Mori (1955), previously described by Kumar and Pritchard (1992). Third stage infective larvae were harvested, concentrated and resuspended in 50 mM Na 2 HPO4, 70 mM NaCl, 15 mM KH 2 PO4, pH 7.4 for immediate use.…”
Section: Preparation Of Third Stage Infective Larvaementioning
confidence: 99%