Complete Genome Sequence of a Newcastle Disease Genotype XIII Virus Isolated from Indigenous Chickens in Zambia

Abolnik, Célia; Mubamba, Chrisborn; Dautu, George; Gummow, Bruce

doi:10.1128/genomea.00841-17

Cited by 13 publications

(14 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Genotype VIIh was also confirmed in commercial brown‐egg layers near Ndola in October 2016. However, a genotype XIII strain was also isolated from diseased indigenous backyard chickens in November 2015 (Abolnik, Mubamba, Dautu, & Gummow, ); thus, the outbreaks that occurred during this period cannot be attributed to solely genotype VIIh.…”

Section: Resultsmentioning

confidence: 99%

Tracing the origins of genotype VIIh Newcastle disease in southern Africa

Abolnik

Mubamba

Wandrag

et al. 2017

Transbound Emerg Dis

View full text Add to dashboard Cite

It is widely accepted that Newcastle disease is endemic in most African countries, but little attention has been afforded to establishing the sources and frequency of the introductions of exotic strains. Newcastle disease outbreaks have a high cost in Africa, particularly on rural livelihoods. Genotype VIIh emerged in South-East Asia and has since caused serious outbreaks in poultry in Malaysia, Indonesia, southern China, Vietnam and Cambodia. Genotype VIIh reached the African continent in 2011, with the first outbreaks reported in Mozambique. Here, we used a combination of phylogenetic evidence, molecular dating and epidemiological reports to trace the origins and spread of subgenotype VIIh Newcastle disease in southern Africa. We determined that the infection spread northwards through Mozambique, and then into the poultry of the north-eastern provinces of Zimbabwe. From Mozambique, it also reached neighbouring Malawi and Zambia. In Zimbabwe, the disease spread southward towards South Africa and Botswana, causing outbreaks in backyard chickens in early-to-mid 2013. In August 2013, the disease entered South Africa's large commercial industry, and the entire country was infected within a year, likely through fomites and the movements of cull chickens. Illegal poultry trading or infected waste from ships and not wild migratory birds was the likely source of the introduction to Mozambique in 2011.

show abstract

Section: Resultsmentioning

confidence: 99%

Tracing the origins of genotype VIIh Newcastle disease in southern Africa

Abolnik

Mubamba

Wandrag

et al. 2017

Transbound Emerg Dis

View full text Add to dashboard Cite

show abstract

“…All 11 studied NDVs belong to genotype XIII. Genotype XIII viruses are widely distributed and have been reported on at least three continents [21, 24, 25, 49–51]. These viruses have historically been classified into five sub-genotypes (XIII a–e) [18, 20, 22–24, 52].…”

Section: Discussionmentioning

confidence: 99%

“…All 11 studied NDVs belong to genotype XIII. Genotype XIII viruses are widely distributed and have been reported on at least three continents [21,24,25,[49][50][51]. *The number of base substitutions per site from averaging all sequence pairs between sub-genotypes of genotype XIII and newly studied groups is shown.…”

Section: Discussionmentioning

confidence: 99%

Genetic and biological characterization of Newcastle disease viruses circulating in Bangladesh during 2010–2017: further genetic diversification of class II genotype XIII in Southcentral Asia

Nooruzzaman

Mumu

Kabiraj

et al. 2021

Journal of General Virology

View full text Add to dashboard Cite

Newcastle disease virus (NDV) is endemic in Bangladesh and is a major threat to commercial poultry operations. While complete fusion (F) genes are recommended for molecular characterization and classification of NDV isolates, heretofore, only partial F gene data have been available for Bangladeshi NDVs. To this end, we obtained the full-length F gene coding sequences of 11 representative NDVs isolated in Bangladesh between 2010 and 2017. In addition, one of the viruses (MK934289/chicken/Bangladesh/C161/2010) was used in an experimental infection of chickens to establish the viral pathotype and study gross and microscopic lesions. Phylogenetic analysis provided evidence that all studied Bangladeshi isolates belong to genotype XIII.2 of class II NDVs. Six of the viruses were isolated between 2010 and 2017 and grouped together with isolates from neighbouring India during 2013–2016. Another four Bangladeshi isolates (2010–2016) formed a separate monophyletic branch within XIII.2 and showed high nucleotide distance from the isolates from India and the other six Bangladeshi viruses within the sub-genotype; however, none of these groups fulfils all classification criteria to be named as a separate sub-genotype. The eleventh Bangladeshi virus studied here (C162) was genetically more distant from the remaining isolates. It out-grouped the viruses from sub-genotypes XIII.2.1 and XIII.2.2 and showed more than 9.5 % nucleotide distance from all genotype XIII sub-genotypes. This isolate may represent an NDV variant that is evolving independently from the other viruses in the region. The experimental infection in chickens revealed that the tested isolate (C161) is a velogenic viscerotropic virus. Massive haemorrhages, congestion and necrosis in different visceral organs, and lymphoid depletion in lymphoid tissues, typical for infection with velogenic NDV, were observed. Our findings demonstrate the endemic circulation of sub-genotype XIII.2 in Southcentral Asia and further genetic diversification of these viruses in Bangladesh and neighbouring India. This constant evolution of the viruses may lead to the establishment of new genetic groups in the region. Additional historical and prospective virus and surveillance data from the region and neighbouring countries will allow a more detailed epidemiological inference.

show abstract

“…Haemagglutination inhibition (HI) assay or enzyme-linked immunosorbent assay (ELISA) are commonly used 3 to 4 weeks post vaccination for assessment of the immune status of animal or flock [10][11][12][13]. Antibodies appear in serum and mucosal membranes 4 to 10 days post immunization (dpi) [13] and reach peak levels at two to three weeks p.i.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Chicken Immune Responses to Immunization with Vaccine La Sota or ZG1999HDS Strain of Newcastle Disease Virus

Nedeljković¹,

Mazija

Cvetić

et al. 2022

Life

View full text Add to dashboard Cite

Newcastle disease (ND) is a highly contagious avian disease. Global control of ND is mainly based on vaccination of poultry; however, reported outbreaks of ND in vaccinated flocks indicate a constant need to re-evaluate the existing vaccines and a development of the new ones. In this study, 4-week-old male chickens of the layer commercial hybrid were immunized oculonasally with a commercial NDV live La Sota vaccine (LS group), a suspension of lyophilized NDV strain ZG1999HDS (ZG group), or saline (Control (K) group). Antibody response was determined by haemagglutination inhibition (HI) assay. Cell-mediated immunity (CMI) was characterized by immunophenotyping of leukocyte’s and T-lymphocyte’s subpopulations (flow cytometry). Applied NDV strains did not cause any adverse reaction in treated chickens. Both strains induced the significantly higher HI antibody response in comparison to the control group, and overall antibody titer was higher in ZG group than in LS group. CMI, manifested as a higher proliferation of B- and T-helper cells, yielded better results in the ZG groups than in the LS group. Based on the obtained results, we conclude that the strain ZG1999HDS is immunogenic and is a suitable candidate for further research and development of poultry vaccines.

show abstract

Complete Genome Sequence of a Newcastle Disease Genotype XIII Virus Isolated from Indigenous Chickens in Zambia

Abstract: The first complete genome sequence of an African-origin Newcastle disease virus belonging to genotype XIII is described here. The virulent strain chicken/Zambia/Chiwoko/2015 was isolated from diseased chickens in 2015.

Cited by 13 publications

References 6 publications

Tracing the origins of genotype VIIh Newcastle disease in southern Africa

Tracing the origins of genotype VIIh Newcastle disease in southern Africa

Genetic and biological characterization of Newcastle disease viruses circulating in Bangladesh during 2010–2017: further genetic diversification of class II genotype XIII in Southcentral Asia

Comparison of Chicken Immune Responses to Immunization with Vaccine La Sota or ZG1999HDS Strain of Newcastle Disease Virus

Contact Info

Product

Resources

About