Complete Genome Sequence of a Burkholderia mallei Isolate Originating from a Glanderous Horse from the Kingdom of Bahrain

Elschner, Mandy C.; Thomas, Prasad; Melzer, Falk

doi:10.1128/genomea.01296-16

Cited by 4 publications

(2 citation statements)

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“…In addition, the majority of the genome sequences in the database belong to strains isolated many decades ago, while only a limited number of genomes are related to recently isolated B . mallei [ 37 – 39 ]. In the current study, 37 additional B .…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity and spatial distribution of Burkholderia mallei by core genome-based multilocus sequence typing analysis

et al. 2022

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Burkholderia mallei is the etiological agent of glanders, a highly contagious and often fatal disease in equids. Due to the high genetic clonality of B. mallei, high-resolution typing assays are necessary to differentiate between individual strains. Here we report on the development and validation of a robust and reproducible core genome-based Multi Locus Sequence Typing Assay (cgMLST) for B. mallei, which is based on 3328 gene targets and enables high-resolution typing at the strain level. The assay was validated using a set of 120 B. mallei genomes from public databases and 23 newly sequenced outbreak strains from in-house strain collections. In this cgMLST analysis, strains from different geographic regions were clearly distinguished by at least 70 allele differences, allowing spatial clustering while closely related and epidemiologically related strains were separated by only zero to three alleles. Neither the different sequencing technologies nor the assembly strategies had an influence on the cgMLST results. The developed cgMLST is highly robust, reproducible and can be used for outbreak investigations, source tracking and molecular characterization of new B. mallei isolates.

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Section: Discussionmentioning

confidence: 99%

Genetic diversity and spatial distribution of Burkholderia mallei by core genome-based multilocus sequence typing analysis

et al. 2022

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“…Glanders has been eradicated from Europe and North America; but countries in Southeast Asia, the Middle East, and South America have experienced outbreaks within the past 25 y. 2,6,7,9,12,13,18,20,22,24 Disease-free status is maintained through stringent testing of all equids prior to importation. Although the “gold standard” test is culture and isolation of the bacteria from infected animals, this method lacks sensitivity and is not appropriate for the high-speed and high-throughput testing demanded for international trade of equids.…”

mentioning

confidence: 99%

Development and validation of a chemiluminescent western blot assay for glanders (Burkholderia mallei) serodetection

Grause,

Elschner,

Ledesma

et al. 2024

J VET Diagn Invest

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Glanders, caused by Burkholderia mallei, is a zoonotic disease of equids. Serologic testing for glanders is required by disease-free countries before international movement of equids. The World Organisation for Animal Health Terrestrial Manual recommends the complement fixation test (CFT) for clearance of individual animals for movement, but the CFT is prone to false-positive results. A colorimetric western blot (WB) assay was developed and validated to resolve false-positive CFT results; however, that assay is relatively time-consuming, and the interpretation is subjective. We present here a procedurally similar chemiluminescent WB assay that performs comparably to the validated colorimetric WB assay and offers noticeable benefits of decreased time-to-result and greater ease of interpretation.

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glanders

2020

CABI Compendium

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Complete Genome Sequence of a Burkholderia mallei Isolate Originating from a Glanderous Horse from the Kingdom of Bahrain

Cited by 4 publications

References 7 publications

Genetic diversity and spatial distribution of Burkholderia mallei by core genome-based multilocus sequence typing analysis

Genetic diversity and spatial distribution of Burkholderia mallei by core genome-based multilocus sequence typing analysis

Development and validation of a chemiluminescent western blot assay for glanders (Burkholderia mallei) serodetection

glanders

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