Complete genome sequence of bacteriophage T5

Wang, Jianbin; Jiang, Yan; Vincent, Myriam; Sun, Yongqiao; Yu, Hong; Wang, Jing; Bao, Qiyu; Kong, Huimin; Hu, Songnian

doi:10.1016/j.virol.2004.10.049

Cited by 115 publications

(125 citation statements)

References 90 publications

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“…[55][56][57] Our finding that CEV2 is a close relative of T5 is advantageous; T5-like phages are exclusively lytic and none have been found to encode pathogenicity islands, virulence/antibiotic resistance genes or integrases. 49,58 To our knowledge, CEV2 is the first T5-like phage to be isolated that infects E. coli O157:H7. Host selectivity is another important parameter in the selection of phages to include in therapeutic cocktails.…”

Section: Discussionmentioning

confidence: 99%

Naturally resident and exogenously applied T4-like and T5-like bacteriophages can reduceEscherichia coliO157

et al. 2011

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Section: Discussionmentioning

confidence: 99%

Naturally resident and exogenously applied T4-like and T5-like bacteriophages can reduceEscherichia coliO157

et al. 2011

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“…The predicted ORFs were highly homologous to those of T5 (20) or T5-like phages (7,9) in the Siphoviridae family. Interestingly, this genome contains redundant 12,854-bp terminal repeats at both ends.…”

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confidence: 99%

Complete Genome Sequence of Pectobacterium carotovorum subsp. carotovorum Bacteriophage My1

Lee

Shin

et al. 2012

J Virol

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c Pectobacterium carotovorum subsp. carotovorum, a member of the Enterobacteriaceae family, is an important plant-pathogenic bacterium causing significant economic losses worldwide. P. carotovorum subsp. carotovorum bacteriophage My1 was isolated from a soil sample. Its genome was completely sequenced and analyzed for the development of an effective biological control agent. Sequence and morphological analyses revealed that phage My1 is a T5-like bacteriophage and belongs to the family Siphoviridae. To date, there is no report of a Pectobacterium-targeting siphovirus genome sequence. Here, we announce the complete genome sequence of phage My1 and report the results of our analysis.

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“…Its recently sequenced genome (GenBank TM accession numbers AY587007, AY692264, and AY543070 and Ref. 16) which is the largest among the T-odd viruses carries singlestranded interruptions at genetically defined positions on one of the DNA strands as well as large terminal redundancies in the form of 10,160-bp direct repeats (17). Another distinctive feature of T5 is the two-step transport of its genome (18).…”

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confidence: 99%

Phage T5 Straight Tail Fiber Is a Multifunctional Protein Acting as a Tape Measure and Carrying Fusogenic and Muralytic Activities

Boulanger

Jacquot

Plançon

et al. 2008

Journal of Biological Chemistry

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We report a bioinformatic and functional characterization of Pb2, a 121-kDa multimeric protein that forms phage T5 straight fiber and is implicated in DNA transfer into the host. Pb2 was predicted to consist of three domains. Region I (residues 1-1030) was mainly organized in coiled coil and shared features of tape measure proteins. Region II (residues 1030 -1076) contained two ␣-helical transmembrane segments. Region III (residues 1135-1148) included a metallopeptidase motif. A truncated version of Pb2 (Pb2-Cterm, residues 964 -1148) was expressed and purified. Pb2-Cterm shared common features with fusogenic membrane polypeptides. It formed oligomeric structures and inserted into liposomes triggering their fusion. Pb2-Cterm caused ␤-galactosidase release from Escherichia coli cells and in vitro peptidoglycan hydrolysis. Based on these multifunctional properties, we propose that binding of phage T5 to its receptor triggers large conformational changes in Pb2. The coiled coil region would serve as a sensor for triggering the opening of the head-tail connector. The C-terminal region would gain access to the host envelope, permitting the local degradation of the peptidoglycan and the formation of the DNA pore by fusion of the two membranes.The mechanism by which the double-stranded DNA of tailed phages is transported through the envelope of Gram-negative bacteria is a complex process for which phages have developed diverse strategies. Transport may depend on transcription (T7) (1), on phage-encoded proteins (T7, T5, phi29) (1-3), and on host membrane potential (T4) (4, 5). The rate of DNA transport may also vary from phage to phage, reaching values as high as 3000 -4000 bp/s (T4) or as low as 800 bp/s (T7) (reviewed in Refs. 6 -8). Renewed attention was recently brought to these processes with studies focusing on the role of the capsid internal pressure as a trigger of DNA ejection (9) and on the structure of tail proteins participating in DNA ejection (10, 11) and with the development of single phage particles studies (12, 13). However, information on the in vivo DNA transport mechanism and on the phage/bacterial partners involved is still scarce.The Syphoviridae coliphage T5 is an interesting example of the complex strategies developed by phages to invade bacteria. It consists of a 90-nm large icosahedral capsid containing a 121,750-bp double-stranded DNA and a 250-nm-long flexible tail. The high resolution structure of the T5 particle was recently solved from cryoelectron microscopy images highlighting two unusual characteristics of this phage: the triangulation number of its capsid (t ϭ 13) and the 3-fold symmetry of its tail tube (14). Several other features make T5 an atypical phage (15). Its recently sequenced genome (GenBank TM accession numbers AY587007, AY692264, and AY543070 and Ref. 16) which is the largest among the T-odd viruses carries singlestranded interruptions at genetically defined positions on one of the DNA strands as well as large terminal redundancies in the form of 10,160-bp direct repeats ...

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Complete genome sequence of bacteriophage T5

Cited by 115 publications

References 90 publications

Naturally resident and exogenously applied T4-like and T5-like bacteriophages can reduceEscherichia coliO157

Naturally resident and exogenously applied T4-like and T5-like bacteriophages can reduceEscherichia coliO157

Complete Genome Sequence of Pectobacterium carotovorum subsp. carotovorum Bacteriophage My1

Phage T5 Straight Tail Fiber Is a Multifunctional Protein Acting as a Tape Measure and Carrying Fusogenic and Muralytic Activities

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