Complete Genome Sequence of “Candidatus Portiera aleyrodidarum” BT-QVLC, an Obligate Symbiont That Supplies Amino Acids and Carotenoids to Bemisia tabaci

Santos-García, Diego; Farnier, Pierre-Antoine; Beitia, Francisco J.; Zchori-Fein, Einat; Vavre, Fabrice; Mouton, Laurence; Moya, Andrés; Latorre, Amparo; Silva, Francisco J.

doi:10.1128/jb.01793-12

Cited by 75 publications

(121 citation statements)

References 20 publications

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“…3), this method should be taken to evaluate the free amino acids in small insect in future. Latest study showed that Portiera was able to synthesize the essential amino acids threonine and tryptophan and the nonessential serine and lack the last gene of phenylalanine, valine, leucine, and isoleucine pathways 35 . Further research is needed to determine the composition of free amino acid in the three B. tabaci Q (cotton, cucumber, and cabbage) populations.…”

Section: Discussionmentioning

confidence: 99%

Relative amount of symbionts in Bemisia tabaci (Gennadius) Q changes with host plant and establishing the method of analyzing free amino acid in B. tabaci

Pan

Jiao

et al. 2013

Communicative & Integrative Biology

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The impact of symbionts on their insect hosts depends on their infection density. In the current study, we investigated the effects of host plants (cucumber, cabbage, and cotton) on the relative amount of symbionts Portiera and Hamiltonella in the whitefly Bemisia tabaci (Gennadius) Q. The relative amounts of symbionts in 3 host plant B. tabaci Q populations with the same genetic background were evaluated by quantitative PCR. The whiteflies of cabbage population harbored more Portiera than those of cucumber and cotton populations, and the relative amount of Portiera did not differ statistically between cotton and cucumber populations. The whiteflies of cucumber and cabbage populations harbored more Hamiltonella than that of cotton population, and the relative amount of Hamiltonella did not differ statistically between cabbage and cucumber populations, indicated that the relative amount of symbionts was significantly affected by host plant. In addition, the method of analyzing the composition of free amino acid in B. tabaci was established. Twenty-eight amino acids were detected in the B. tabaci Q population, the non-essential amino acids, such as glutamate, glutamine, alanine, proline and the essential amino acid arginine were the dominant amino acids in B. tabaci Q.

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Section: Discussionmentioning

confidence: 99%

Relative amount of symbionts in Bemisia tabaci (Gennadius) Q changes with host plant and establishing the method of analyzing free amino acid in B. tabaci

Pan

Jiao

et al. 2013

Communicative & Integrative Biology

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“…tabaci host species complex were obtained from GenBank (Santos-Garcia et al 2012; Sloan and Moran 2012b; Jiang et al 2013), including two sequences from B. tabaci biotype B ( Portiera BT-B) and two from biotype Q ( Portiera BT-Q). Two additional whitefly collections were made in June 2012.…”

Section: Methodsmentioning

confidence: 99%

“…Here, we take advantage of the availability of additional Portiera genomes that were independently sequenced from different populations and biotypes in the B . tabaci species complex (Santos-Garcia et al 2012; Jiang et al 2013). We also report the complete Portiera genome from the distantly related host species Trialeurodes vaporarium .…”

Section: Introductionmentioning

confidence: 99%

The Evolution of Genomic Instability in the Obligate Endosymbionts of Whiteflies

Sloan

Moran

2013

Genome Biology and Evolution

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Many insects depend on ancient associations with intracellular bacteria to perform essential metabolic functions. These endosymbionts exhibit striking examples of convergence in genome architecture, including a high degree of structural stability that is not typical of their free-living counterparts. However, the recently sequenced genome of the obligate whitefly endosymbiont Portiera revealed features that distinguish it from other ancient insect associates, such as a low gene density and the presence of perfectly duplicated sequences. Here, we report the comparative analysis of Portiera genome sequences both within and between host species. In one whitefly lineage (Bemisia tabaci), we identify large-scale structural polymorphisms in the Portiera genome that exist even within individual insects. This variation is likely mediated by recombination across identical repeats that are maintained by gene conversion. The complete Portiera genome sequence from a distantly related whitefly host (Trialeurodes vaporarium) confirms a history of extensive genome rearrangement in this ancient endosymbiont. Using gene-order-based phylogenetic analysis, we show that the majority of rearrangements have occurred in the B. tabaci lineage, coinciding with an increase in the rate of nucleotide substitutions, a proliferation of short tandem repeats (microsatellites) in intergenic regions, and the loss of many widely conserved genes involved in DNA replication, recombination, and repair. These results indicate that the loss of recombinational machinery is unlikely to be the cause of the extreme structural conservation that is generally observed in obligate endosymbiont genomes and that large, repetitive intergenic regions are an important substrate for genomic rearrangements.

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“…These analyses indicated that these strains have a 351-kb circular molecule and the genomes of the two groups share around 99.8 % identity (Jiang et al 2013). Even though displaying almost identical gene repertoire and order, some species-specific genetic differences were identified (Santos-Garcia et al 2012;Jiang et al 2013).…”

Section: Introductionmentioning

confidence: 95%

“…"Ca. Portiera"wasfound tobeamajorsuppliertoitsinsecthostdietof essential amino acids (Jiang et al 2013) and carotenoids (Santos-Garcia et al 2012). Endosymbiotic associations may also be involved in modulating host immunocompetence variation (Braquart-Varnier et al 2008).…”

Section: Introductionmentioning

confidence: 98%

Fluorescence in situ hybridization analysis of endosymbiont genera reveals novel infection patterns in a tomato-infesting Bemisia tabaci population from Brazil

Blawid

Morgado

Souza

et al. 2015

Trop. plant pathol.

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The Bemisia tabaci cryptic species complex harbors a diversified flora of primary and secondary endosymbionts, which plays crucial roles in many aspects of the insect biology. The endosymbiont infection pattern is dependent upon many factors, including host plant and geographic origin. In Brazil, the invasion of B. tabaci Middle East-Asia Minor-1 (MEAM 1) populations was observed in tomato (Solanum lycopersicum L.) fields in the 1990s, which was followed by severe begomovirus epidemics. Here we confirmed the vertical transmission of "Candidatus Portiera" and the localization of secondary endosymbionts in distinct B. tabaci developmental stages. Hamiltonella defensa was detected in bacteriocytes but also scattered in the leg muscles as well as in the male heads. Wolbachia-specific signals were found in eggs, nymphs, male adults, and female gonads in predominant association with bacteriocytes. These results were somewhat surprising since Wolbachia infection is rarely found in MEAM 1 populations from the Old World. Our results reinforce the notion that endosymbiont infection pattern may vary according to the insect population, gender, developmental stage, and geographic origin. This characterization will provide tools to study the endosymbiont function in the transmission ability/efficiency of a complex of tomato-infecting bipartite begomoviruses by B. tabaci under Neotropical conditions.

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Complete Genome Sequence of “Candidatus Portiera aleyrodidarum” BT-QVLC, an Obligate Symbiont That Supplies Amino Acids and Carotenoids to Bemisia tabaci

Cited by 75 publications

References 20 publications

Relative amount of symbionts in Bemisia tabaci (Gennadius) Q changes with host plant and establishing the method of analyzing free amino acid in B. tabaci

Relative amount of symbionts in Bemisia tabaci (Gennadius) Q changes with host plant and establishing the method of analyzing free amino acid in B. tabaci

The Evolution of Genomic Instability in the Obligate Endosymbionts of Whiteflies

Fluorescence in situ hybridization analysis of endosymbiont genera reveals novel infection patterns in a tomato-infesting Bemisia tabaci population from Brazil

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